Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Endophytic Bacteria Improve Root Traits, Biomass and Yield of Helianthus tuberosus L. under Normal and Deficit Water Conditions

  • Namwongsa, Junthima (Department of Microbiology, Faculty of Science, Khon Kaen University) ;
  • Jogloy, Sanun (Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University) ;
  • Vorasoot, Nimitr (Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University) ;
  • Boonlue, Sophon (Department of Microbiology, Faculty of Science, Khon Kaen University) ;
  • Riddech, Nuntavan (Department of Microbiology, Faculty of Science, Khon Kaen University) ;
  • Mongkolthanaruk, Wiyada (Department of Microbiology, Faculty of Science, Khon Kaen University)
  • Received : 2019.03.29
  • Accepted : 2019.09.05
  • Published : 2019.11.28
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Abstract

Drought is more concerned to be a huge problem for agriculture as it affects plant growth and yield. Endophytic bacteria act as plant growth promoting bacteria that have roles for improving plant growth under stress conditions. The properties of four strains of endophytic bacteria were determined under water deficit medium with 20% polyethylene glycol. Bacillus aquimaris strain 3.13 showed high 1-aminocyclopropane-1-carboxylate (ACC) deaminase production; Micrococcus luteus strain 4.43 produced indole acetic acid (IAA). Exopolysaccharide production was high in Bacillus methylotrophicus strain 5.18 while Bacillus sp. strain 5.2 did not show major properties for drought response. Inoculation of endophytic bacteria into plants, strain 3.13 and 4.43 increased height, shoot and root weight, root length, root diameter, root volume, root area and root surface of Jerusalem artichoke grown under water limitation, clearly shown in water supply at 1/3 of available water. These increases were caused by bacteria ACC deaminase and IAA production; moreover, strain 4.43 boosted leaf area and chlorophyll levels, leading to increased photosynthesis under drought at 60 days of planting. The harvest index was high in the treatment with strain 4.43 and 3.13 under 1/3 of available water, promoting tuber numbers and tuber weight. Inulin content was unchanged in the control between well-watered and drought conditions. In comparison, inulin levels were higher in the endophytic bacteria treatment under both conditions, although yields dipped under drought. Thus, the endophytic bacteria promoted in plant growth and yield under drought; they had outstanding function in the enhancement of inulin content under well-watered condition.

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References

  1. Mengel K. 1982. Factors of plant nutrient availability relevant to soil testing. Soil 64: 129-138.
  2. Mahajan S, Tuteja N. 2005. Cold, salinity and drought stresses: an overview. Arch. Biochem. Biophys. 444: 139-158. https://doi.org/10.1016/j.abb.2005.10.018
  3. Aduldecha C, Kaewpradit W, Vorasoot N, Puangbut D, Jogloy S, Patanothai A. 2016. Effects of water regimes on inulin content and inulin yield of Jerusalem artichoke genotypes with different levels of drought tolerance. Turk. J. Agric. For. 40: 335-343. https://doi.org/10.3906/tar-1506-39
  4. Vurukonda SS, Vardharajula S, Shrivastava M, SkZ A. 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiol. Res. 184: 13-24. https://doi.org/10.1016/j.micres.2015.12.003
  5. Du H, Wu N, Fu J, Wang S, Li X, Xiao J, et al. 2012. A GH3 family member, OsGH3-2, modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice. J. Exp. Bot. 63: 6467-6480. https://doi.org/10.1093/jxb/ers300
  6. Glick BR, Cheng Z, Czarny J, Duan J. 2007. Promotion of plant growth by ACC deaminase-producing soil bacteria. Eur. J. Plant Pathol. 119: 329-339. https://doi.org/10.1007/s10658-007-9162-4
  7. Wilkinson JF. 1958. The extracellular polysaccharides of bacteria. Bacteriol. Rev. 22: 46-73. https://doi.org/10.1128/MMBR.22.1.46-73.1958
  8. Hepper CM. 1975. Extracellular polysaccharides of soil bacteria, pp. 93-111. In Walker N (ed.), Soil microbiology, a critical review, Wiley, New York.
  9. Khamwan S, Boonlue S, Riddech N, Jogloy S, Mongkolthanaruk W. 2018. Characterization of endophytic bacteria and their response to plant growth promotion in Helianthus tuberosus L. Biocatal. Agric. Biotechnol. 13: 153?159. https://doi.org/10.1016/j.bcab.2017.12.007
  10. Ali SZ, Sandhya V, Rao LV. 2014. Isolation and characterization of drought-tolerant ACC deaminase and exopolysaccharide-producing fluorescent Pseudomonas sp. Ann. Microbiol. 64: 493-502. https://doi.org/10.1007/s13213-013-0680-3
  11. Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356. https://doi.org/10.1021/ac60111a017
  12. Bates LS, Waldren RP, Teare ID. 197 3. Rapid determination of free proline for water-stress studies. Plant Soil 39: 205-207. https://doi.org/10.1007/BF00018060
  13. Doorenbos J, Pruitt WO. 1992. Crop water requirement: calculation of crop water requirement, pp. 1-65. In Brouwer C, Heibloem M (eds.), Irrigation water management training manual no. 3, FAO of The United Nation, Italy.
  14. Janket A, Jogloy S, Vorasoot N, Kesmala T, Holbrook C, Patanothai A. 2013. Genetic diversity of water use efficiency in Jerusalem artichoke (Helianthus tuberosus L.) germplasm. Aust. J. Crop Sci. 7: 1670-1681.
  15. Saengkanuk A, Nuchadomrong S, Jogloy S, Patanothai A, Srijaranai S. 2011. A simplified spectrophotometric method for the determination of inulin in Jerusalem artichoke (Helianthus tuberosus L.) tubers. Eur. Food Res. Technol. 233: 609. https://doi.org/10.1007/s00217-011-1552-3
  16. Namwongsa J, Boonlue S, Riddech N, Jogloy S, Mongkolthanaruk, W. 2018. The survival of endophytic bacteria isolated from Jerusalem artichoke in drought conditions. Int. J. Appl. Phys. Sci. 4: 59-68.
  17. Arakawa T, Timasheff SN. 1985. Mechanism of poly(ethylene glycol) interaction with proteins. Biochemistry 24: 6756-6762. https://doi.org/10.1021/bi00345a005
  18. Singh RP, Shelke GM, Kumar A, Jha PN. 2015. Biochemistry and genetics of ACC deaminase: a weapon to "stress ethylene" produced in plants. Front. Microbiol. 6: 937. https://doi.org/10.3389/fmicb.2015.00937
  19. Li Z, Chang S, Ye S, Chen M, Lin L, Li Y, et al. 2015. Differentiation of 1-aminocyclopropane-1-carboxylate (ACC) deaminase from its homologs is the key for identifying bacteria containing ACC deaminase. FEMS Microbiol. Ecol. 91: doi: 10.1093/femsec/fiv112
  20. Roberson EB, Firestone MK. 1992. Relationship between desiccation and exopolysaccharide production in soil Pseudomonas sp. Appl. Environ. Microbiol. 58: 1284-1291. https://doi.org/10.1128/aem.58.4.1284-1291.1992
  21. Bashan Y, Holguin G, de-Bashan LE. 2004. Azospirillumplant relationships: physiological, molecular, agricultural, and environmental advances. Can. J. Microbiol. 50: 521-577. https://doi.org/10.1139/w04-035
  22. Jaleel CA, Manivannan P, Sankar B, Kishorekumar A, Gopi R, Somasundaram R, et al. 2007. Pseudomonas fluorescens enhances biomass yield and ajmalicine production in Catharanthus roseus under water deficit stress. Colloids Surf. B. Biointerfaces. 60: 7-11. https://doi.org/10.1016/j.colsurfb.2007.05.012
  23. Kogut M, Russell NJ. 1987. Life at the limits: considerations on how bacteria can grow at extremes of temperature and pressure, or with high concentrations of ions and solutes. Sci. Prog. 71: 381-399.
  24. Ruttanaprasert R, Jogloy S, Vorasoot N, Kesmala T, Kanwar RS, Holbrook CC, et al. 2015. Root responses of Jerusalem artichoke genotypes to different water regimes. Biomass Bioenergy 81: 369-377. https://doi.org/10.1016/j.biombioe.2015.07.027
  25. Olanrewaju OS, Glick BR, Babalola OO. 2017. Mechanisms of action of plant growth promoting bacteria. World J. Microbiol. Biotechnol. 33: 197. https://doi.org/10.1007/s11274-017-2364-9
  26. Bolouri-Moghaddam MR, Le RK, Rolland F, Van den Ende W. 2010. Sugar signaling and antioxidant network connections in plant cells. FEBS J. 277: 202-207.
  27. Bianco C, Imperlini E, Calogero R, Senatore B, Pucci P, Defez R. 2006. Indole-3-acetic acid regulates the central metabolic pathways in Escherichia coli. Microbiology 152: 2421-2431. https://doi.org/10.1099/mic.0.28765-0
  28. Vandoorne B, Mathieu AS, Van den Ende W, Vergauwen R, Perilleux C, Javaux M, et al. 2012. Water stress drastically reduces root growth and inulin yield in Cichorium intybus (var. sativum) independently of photosynthesis. J. Exp. Bot. 63: 4359-4373. https://doi.org/10.1093/jxb/ers095
  29. Monti A, Amaducci MT, Pritoni G, Venturi G. 2005. Growth, fructan yield, and quality of chicory (Cichorium intybus L.) as related to photosynthetic capacity, harvest time and water regime. J. Exp. Bot. 56: 1389-1395. https://doi.org/10.1093/jxb/eri140
  30. Van den Ende W, De Coninck B, Van Laere A. 2004. Plant fructan exohydrolases: a role in signaling and defense? Trends Plant. Sci. 9: 523-528. https://doi.org/10.1016/j.tplants.2004.09.008
  31. Khamwan S, Mongkolthanaruk W. 2016. Exploring of the inulin synthesis gene of endophytic bacteria by the new degenerated primers. pp. 24-27. 6th Annual International Conference on Advances in Biotechnology Proceedings.

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