The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Identification of New Isolates of Phytophthora sojae and the Reactions of Korean Soybean Cultivars Following Hypocotyl Inoculation

  • Kang, In Jeong (Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science, National Institute of Crop Science) ;
  • Kang, Sunjoo (Department of Crop Science, Chungnam National University) ;
  • Jang, Ik Hyun (Department of Crop Science, Chungnam National University) ;
  • Jang, Yun Woo (Department of Southern Area Crop Science, National Institute of Crop Science) ;
  • Shim, Hyung Kwon (Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science, National Institute of Crop Science) ;
  • Heu, Sunggi (Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science, National Institute of Crop Science) ;
  • Lee, Sungwoo (Department of Crop Science, Chungnam National University)
  • 투고 : 2019.09.19
  • 심사 : 2019.11.04
  • 발행 : 2019.12.01
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초록

Phytophthora root and stem rot (PRSR) caused by Phytophthora sojae is one of the most destructive diseases of soybean. PRSR recently became an issue as soybean cultivation in paddy fields increased in South Korea. The management of PRSR mainly involves R-gene-mediated resistance, however, little is known about the resistance in Korean cultivars. Major Korean soybean cultivars were investigated for the presence or absence of R-gene-mediated resistance to four P. sojae isolates, two of which were new isolates. Isolate-specific reactions were observed following P. sojae inoculation. Of 21 cultivars, 15-20 cultivars (71.4-95.2%) showed susceptible reaction for each isolate. Ten cultivars were susceptible to all the isolates, and six cultivars were identified to have R-gene-mediated resistance to one or two isolates. The results of this study would provide a framework for the discovery of resistant cultivars, development of new cultivars resistant to P. sojae, and investigation of pathogenic diversity of P. sojae population in South Korea.

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참고문헌

  1. Abney, T. S., Melgar, J. C., Richards, T. L., Scott, D. H., Grogan, J. and Young, J. 1997. New races of Phytophthora sojae with Rps1-d virulence. Plant Dis. 81:653-655. https://doi.org/10.1094/PDIS.1997.81.6.653
  2. Akamatsu, H., Kato, M., Ochi, S., Mimuro, G., Matsuoka, J.-I. and Takahashi, M. 2019. Variation in the resistance of Japanese soybean cultivars to Phytophthora root and stem rot during the early plant growth stages and the effects of a fungicide seed treatment. Plant Pathol. J. 35:219-233. https://doi.org/10.5423/ppj.oa.11.2018.0252
  3. Allen, T. W., Bradley, C. A., Sisson, A. J., Byamukama, E., Chilvers, M. I., Coker, C. M., Collins, A. A., Damicone, J. P., Dorrance, A. E., Dufault, N. S., Esker, P. D., Faske, T. R., Giesler, L. J., Grybauskas, A. P., Hershman, D. E., Hollier, C. A., Isakeit, T., Jardine, D. J., Kelly, H. M., Kemerait, R. C., Kleczewski, N. M., Koenning, S. R., Kurle, J. E., Malvick, D. K., Markell, S. G., Mehl, H. L., Mueller, D. S., Mueller, J. D., Mulrooney, R. P., Nelson, B. D., Newman, M. A., Osborne, L., Overstreet, C., Padgett, G. B., Phipps, P. M., Price, P. P., Sikora, E. J., Smith, D. L., Spurlock, T. N., Tande, C. A., Tenuta, A. U., Wise, K. A. and Wrather, J. A. 2017. Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014. Plant Health Prog. 18:19-27. https://doi.org/10.1094/PHP-RS-16-0066
  4. Anderson, T. R. and Buzzell, R. I. 1992. Inheritance and linkage of the Rps7 gene for resistance to Phytophthora rot of soybean. Plant Dis. 76:958-959. https://doi.org/10.1094/PD-76-0958
  5. Athow, K. L. and Laviolette, F. A. 1982. Rps 6, a major gene for resistance to Phytophthora megasperma f. sp. glycinea in soybean. Phytopathology 72:1564-1567. https://doi.org/10.1094/Phyto-72-1564
  6. Athow, K. L., Laviolette, F. A., Mueller, E. H. and Wilcox, J. R. 1980. A new major gene for resistance to Phytophthoramegasperma var sojae in soybean. Phytopathology 70:977-980. https://doi.org/10.1094/Phyto-70-977
  7. Bienapfl, J. C., Malvick, D. K. and Percich, J. A. 2011. Specific molecular detection of Phytophthora sojae using conventional and real-time PCR. Fungal Biol. 115:733-740. https://doi.org/10.1016/j.funbio.2011.05.007
  8. Burnham, K. D., Dorrance, A. E., Francis, D. M., Fioritto, R. J. and Martin, S. K. S. 2003. Rps8, a new locus in soybean for resistance to Phytophthora sojae. Crop Sci. 43:101-105. https://doi.org/10.2135/cropsci2003.1010
  9. Buzzell, R. I. and Anderson, T. R. 1992. Inheritance and race reaction of a new soybean Rps1 allele. Plant Dis. 76:600-601. https://doi.org/10.1094/PD-76-0600
  10. Cheng, Y., Ma, Q., Ren, H., Xia, Q., Song, E., Tan, Z., Li, S., Zhang, G. and Nian, H. 2017. Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing. Theor. Appl. Genet. 130:1041-1051. https://doi.org/10.1007/s00122-017-2869-5
  11. Demirbas, A., Rector, B. G., Lohnes, D. G., Fioritto, R. J., Graef, G. L., Cregan, P. B., Shoemaker, R. C. and Specht, J. E. 2001. Simple sequence repeat markers linked to the soybean Rps genes for Phytophthora resistance. Crop Sci. 41:1220-1227. https://doi.org/10.2135/cropsci2001.4141220x
  12. Diers, B. W., Mansur, L., Imsande, J. and Shoemaker, R. C. 1992. Mapping Phytophthora resistance loci in soybean with restriction fragment length polymorphism markers. Crop Sci. 32:377-383. https://doi.org/10.2135/cropsci1992.0011183X003200020020x
  13. Dorrance, A. and Grunwald, N. J. 2009. Phytophthora sojae: diversity among and within populations. In: Oomycete genetics and genomics: diversity, interactions, and research tools, eds. by K. Lamour and S. Kamoun, pp. 197-212. John Wiley & Sons, Inc., Hoboken, NJ, USA.
  14. Dorrance, A. E. 2018. Management of Phytophthora sojae of soybean: a review and future perspectives. Can. J. Plant Pathol. 40:210-219. https://doi.org/10.1080/07060661.2018.1445127
  15. Dorrance, A. E., Berry, S. A., Anderson, T. R. and Meharg, C. 2008. Isolation, storage, pathotype characterization, and evaluation of resistance for Phytophthora sojae in soybean. Plant Health Prog. Online publication. DOI: 10.1094/php-2008-0118-01-dg.
  16. Dorrance, A. E., Jia, H. and Abney, T. S. 2004. Evaluation of soybean differentials for their interaction with Phytophthora sojae. Plant Health Prog. Online publication. DOI: 10.1094/PHP-2004-0309-01-RS.
  17. Flor, H. H. 1971. Current status of the gene-for-gene concept. Annu. Rev. Phytopathol. 9:275-296. https://doi.org/10.1146/annurev.py.09.090171.001423
  18. Gordon, S. G., Martin, S. K. S. and Dorrance, A. E. 2006. Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci. 46:168-173. https://doi.org/10.2135/cropsci2004.04-0024
  19. Grau, C. R., Dorrance, A. E., Bond, J. and Russin, J. S. 2004. Fungal diseases. In: Soybeans: improvement, production, and uses, eds. by H. R. Boerma and J. E. Specht, pp. 679-763. American Society of Agronomy. Crop Science Society of America. Soil Science Society of America, Madison, WI, USA.
  20. Huang, J., Guo, N., Li, Y., Sun, J., Hu, G., Zhang, H., Li, Y., Zhang, X., Zhao, J., Xing H. and Qiu, L. 2016. Phenotypic evaluation and genetic dissection of resistance to Phytophthora sojae in the Chinese soybean mini core collection. BMC Genet. 17:85. https://doi.org/10.1186/s12863-016-0383-4
  21. Jee, H. J., Kim, W. G. and Cho, W. D. 1998. Occurrence of Phytophthora root rot on soybean (Glycine max) and identification of the causal fungus. Crop Prot. 40:16-22. https://doi.org/10.1016/j.cropro.2012.03.024
  22. Kaufmann, M. J. and Gerdemann, J. W. 1958. Root and stem rot of soybean caused by Phytophthora sojae n. sp. Phytopathology 48:201-208.
  23. Kilen, T. C., Hartwig, E. E. and Keeling, B. L. 1974. Inheritance of a second major gene for resistance to Phytophthora rot in soybeans. Crop Sci. 14:260-262. https://doi.org/10.2135/cropsci1974.0011183X001400020027x
  24. Koenning, S. R. and Wrather, J. A. 2010. Suppression of soybean yield potential in the continental United States by plant diseases from 2006 to 2009. Plant Health Prog. Online publication. DOI: 10.1094/PHP-2010-1122-01-RS.
  25. Li, Y., Sun, S., Zhong, C., Wang, X., Wu, X. and Zhu, Z. 2017. Genetic mapping and development of co-segregating markers of RpsQ, which provides resistance to Phytophthora sojae in soybean. Theor. Appl. Genet. 130:1223-1233. https://doi.org/10.1007/s00122-017-2883-7
  26. Lin, F., Zhao, M., Ping, J., Johnson, A., Zhang, B., Abney, T. S., Hughes, T. J. and Ma, J. 2013. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B. Theor. Appl. Genet. 126:2177-2185. https://doi.org/10.1007/s00122-013-2127-4
  27. Mideros, S., Nita, M. and Dorrance, A. E. 2007. Characterization of components of partial resistance, Rps2, and root resistance to Phytophthora sojae in soybean. Phytopathology 97:655-662. https://doi.org/10.1094/PHYTO-97-5-0655
  28. Mueller, E. H., Athow, K. L. and Laviolette, F. A. 1978. Inheritance of resistance to four physiologic races of Phytophthora megasperma var. sojae. Phytopathology 68:1318-1322. https://doi.org/10.1094/Phyto-68-1318
  29. Niu, J., Guo, N., Sun, J., Li, L., Cao, Y., Li, S., Huang, J., Zhao, J., Zhao, T. and Xing, H. 2017. Fine mapping of a resistance gene RpsHN that controls Phytophthora sojae using recombinant inbred lines and secondary populations. Front. Plant Sci. 8:538.
  30. Sahoo, D. K., Abeysekara, N. S., Cianzio, S. R., Robertson, A. E. and Bhattacharyya, M. K. 2017. A novel Phytophthora sojae resistance Rps12 gene mapped to a genomic region that contains several Rps genes. PLoS ONE 12:e0169950. https://doi.org/10.1371/journal.pone.0169950
  31. Schmitthenner, A. F. 1985. Problems and progress in control of Phytophthora root rot of soybean. Plant Dis. 69:362-368. https://doi.org/10.1094/PD-69-362
  32. Stewart, S. M. 2011. Characterization and management of genetic diversity in Phytophthora sojae populations. Ph.D. thesis. Iowa State University, Ames, IA, USA.
  33. Su, Y. C. and Shen, C. Y. 1993. The discovery and biological characteristics studies of Phytophthora megasperma f. sp. glycinea on soybean in China. Acta Phytopathol. Sin. 23:341-347 (in Chinese).
  34. Sugimoto, T., Kato, M., Yoshida, S., Matsumoto, I., Kobayashi, T., Kaga, A., Hajika, M., Yamamoto, R., Watanabe, K., Aino, M., Matoh, T., Walker, D. R., Biggs, A. R. and Ishimoto, M. 2012. Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans. Breed. Sci. 61:511-522. https://doi.org/10.1270/jsbbs.61.511
  35. Sun, J., Li, L., Zhao, J., Huang, J., Yan, Q., Xing, H. and Guo, N. 2014. Genetic analysis and fine mapping of RpsJS, a novel resistance gene to Phytophthora sojae in soybean [Glycine max (L.) Merr.]. Theor. Appl. Genet. 127:913-919. https://doi.org/10.1007/s00122-014-2266-2
  36. Sun, S., Wu, X. L., Zhao, J. M., Wang, Y. C., Tang, Q. H., Yu, D. Y., Gai, J. Y. and Xing, H. 2011. Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed. 130:139-143. https://doi.org/10.1111/j.1439-0523.2010.01794.x
  37. Wagner, R. E. and Bernard, R. L. 1991. Response of Williams isolines to eight races of Phytophthora megasperma f. sp. glycinea. Soybean Genet. Newsl. 18:236-239.
  38. Wang, Y., Zhang, W., Wang, Y. and Zheng, X. 2006. Rapid and sensitive detection of Phytophthora sojae in soil and infected soybeans by species-specific polymerase chain reaction assays. Phytopathology 96:1315-1321. https://doi.org/10.1094/PHYTO-96-1315
  39. Weng, C., Yu, K., Anderson, T. R. and Poysa, V. 2001. Mapping genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7. J. Hered. 92:442-446. https://doi.org/10.1093/jhered/92.5.442
  40. Wrather, A. and Koenning, S. 2009. Effects of diseases on soybean yields in the United States 1996 to 2007. Plant Health Prog. Online publication. DOI: 10.1094/PHP-2009-0401-01-RS.
  41. Zhang, J., Xia, C., Wang, X., Duan, C., Sun, S., Wu, X. and Zhu, Z. 2013. Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar. Theor. Appl. Genet. 126:1555-1561. https://doi.org/10.1007/s00122-013-2073-1
  42. Zhang, S., Xu, P., Wu, J., Xue, A. G., Zhang, J., Li, W., Chen, C., Chen, W. and Lv, H. 2010. Races of Phytophthora sojae and their virulences on soybean cultivars in Heilongjiang, China. Plant Dis. 94:87-91. https://doi.org/10.1094/PDIS-94-1-0087