Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Lytic and Highly Specific Phage against Yersinia enterocolitica as a Novel Biocontrol Agent

  • Gwak, Kyoung Min (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Choi, In Young (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Jinyoung (Department of Plant and Food Sciences, Sangmyung University) ;
  • Oh, Jun-Hyun (Department of Plant and Food Sciences, Sangmyung University) ;
  • Park, Mi-Kyung (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2018.08.01
  • Accepted : 2018.09.12
  • Published : 2018.11.28
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Abstract

The aim of this study was to isolate and characterize a lytic Yersinia enterocolitica-specific phage (KFS-YE) as a biocontrol agent. KFS-YE was isolated and purified with the final concentration of ($11.72{\pm}0.03$) log PFU/ml from poultry. As observed by transmission electron microscopy, KFS-YE consisted of an icosahedral head and a contractile tail, and was classified in the Myoviridae family. KFS-YE showed excellent narrow specificity against Y. enterocolitica only. Its lytic activity was stable at wide ranges of pH (4-11) and temperature ($4-50^{\circ}C$). The latent period and burst size of KFS-YE were determined to be 45 min and 38 PFU/cell, respectively. KFS-YE showed relatively robust storage stability at -20, 4, and $22^{\circ}C$ for 40 weeks. KFS-YE demonstrated a bactericidal effect in vitro against Y. enterocolitica and provided excellent efficiency with a multiplicity of infection as low as 0.01. This study demonstrated the excellent specificity, stability, and efficacy of KFS-YE as a novel biocontrol agent. KFS-YE may be employed as a practical and promising biocontrol agent against Y. enterocolitica in food.

Keywords

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