Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effects of Orally-Administered Bifidobacterium animalis subsp. lactis Strain BB12 on Dextran Sodium Sulfate-Induced Colitis in Mice

  • Chae, Jung Min (Department of Food and Biotechnology, Korea University) ;
  • Heo, Wan (Department of Food and Biotechnology, Korea University) ;
  • Cho, Hyung Taek (Department of Food and Biotechnology, Korea University) ;
  • Lee, Dong Hun (Department of Food and Biotechnology, Korea University) ;
  • Kim, Jun Ho (Department of Food Science and Biotechnology, Andong National University) ;
  • Rhee, Min Suk (Department of Biotechnology, Korea University) ;
  • Park, Tae-Sik (Department of Life Science, Gachon University) ;
  • Kim, Yong Ki (Maeil Dairies Co., Ltd R&D Center) ;
  • Lee, Jin Hyup (Department of Food and Biotechnology, Korea University) ;
  • Kim, Young Jun (Department of Food and Biotechnology, Korea University)
  • Received : 2018.05.31
  • Accepted : 2018.09.11
  • Published : 2018.11.28
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Abstract

Inflammatory bowel disease, including Crohn's disease and ulcerative colitis (UC), is a chronically relapsing inflammatory disorder of the gastrointestinal tract. Intestinal epithelial cells (IECs) constitute barrier surfaces and play a critical role in maintaining gut health. Dysregulated immune responses and destruction of IECs disrupt intestinal balance. Dextran sodium sulfate (DSS) is the most widely used chemical for inducing colitis in animals, and its treatment induces colonic inflammation, acute diarrhea, and shortening of the intestine, with clinical and histological similarity to human UC. Current treatments for this inflammatory disorder have poor tolerability and insufficient therapeutic efficacy, and thus, alternative therapeutic approaches are required. Recently, dietary supplements with probiotics have emerged as promising interventions by alleviating disturbances in the indigenous microflora in UC. Thus, we hypothesized that the probiotic Bifidobacterium animalis subsp. lactis strain BB12 could protect against the development of colitis in a DSS-induced mouse model of UC. In the present study, oral administration of BB12 markedly ameliorated DSS-induced colitis, accompanied by reduced tumor necrosis factor-${\alpha}$-mediated IEC apoptosis. These findings indicate that the probiotic strain BB12 can alleviate DSS-induced colitis and suggest a novel mechanism of communication between probiotic microorganisms and intestinal epithelia, which increases intestinal cell survival by modulating pro-apoptotic cytokine expression.

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