Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-inflammatory Effects of Various Mushrooms in LPS-stimulated RAW264.7 Cells

  • Seo, Kyung Hye (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Park, Jeong-Yong (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Noh, Hyung-Jun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Lee, Ji Yeon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Lee, Eun Young (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Han, Jae-Gu (Mushroom Research Division, National Institute of Horticultural & Herbal Science) ;
  • Kim, Jin Hyo (Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Cheong, Mi Sun (Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
  • Received : 2018.05.18
  • Accepted : 2018.07.24
  • Published : 2018.10.31
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Abstract

Mushrooms have been widely cultivated and consumed as foods and herbal medicines owing to their various biological properties. However, few studies have evaluated the anti-inflammatory effects of mushrooms. Here, we investigated the effects of mushroom extracts (MEs) on lipopolysaccharide (LPS)-induced inflammation in macrophages (RAW264.7 cells). First, we extracted MEs with either water or ethanol. Using LPS-treated RAW264.7 cells, we measured cell proliferation and NO production. Gene expression of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), interleukin (IL)-6 (IL-6), and $IL-1{\beta}$ was assessed by RT-PCR, and protein abundance of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) and phosphorylation of p65 were determined by immunoblotting. MEs prepared using both water and ethanol inhibited LPS-induced inflammation in RAW264.7 cells. Nitric oxide (NO) levels induced by LPS were reduced by treatment with MEs. Isaria japonica Yasuda water extracts and Umbilicaria esculenta (Miyoshi) Minks ethanol extracts significantly decreased the mRNA expression of inflammation-related cytokine genes including $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$. Similarly, the protein abundance of iNOS and COX-2 was also decreased. The phosphorylation of p65, a subunit of nuclear $factor-{\kappa}B$ was at least partly suppressed by MEs. This study suggests that mushrooms could be included in the diet to prevent and treat macrophage-related chronic immune diseases.

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References

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