Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets

  • Yoo, Won Gi (Department of Medical Environmental Biology, Chung-Ang University College of Medicine) ;
  • Kim, Sun-Min (Department of Veterinary Parasitology, College of Veterinary Medicine, Seoul National University) ;
  • Won, Eun Jeong (Department of Medicine, Chonnam National University Medical School) ;
  • Lee, Ji-Yun (Department of Medical Environmental Biology, Chung-Ang University College of Medicine) ;
  • Dai, Fuhong (Department of Medical Environmental Biology, Chung-Ang University College of Medicine) ;
  • Woo, Ho Choon (Department of Veterinary Parasitology, College of Veterinary Medicine, Jeju National University) ;
  • Nam, Ho-Woo (Department of Parasitology, College of Medicine, Catholic University) ;
  • Kim, Tae Im (Department of Medical Environmental Biology, Chung-Ang University College of Medicine) ;
  • Han, Jeong-Hee (Department of Veterinary Pathology, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kwak, Dongmi (Department of Veterinary Parasitology, Kyungpook National University College of Veterinary Sciences) ;
  • Cho, Yun Sang (Parasitic and Insect Disease Laboratory, Bacterial and Parasitic Disease Division, Department of Animal and Plant Health Research, Animal and Plant Quarantine Agency) ;
  • Kang, Seung-Won (VexPert Co.) ;
  • Kim, Tong-Soo (Department of Parasitology and Tropical Medicine and Inha Research Institute for Medical Sciences, Inha University School of Medicine) ;
  • Zhu, Xing-Quan (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Wang, Chunren (Department of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University) ;
  • Youn, Heejeong (Department of Veterinary Parasitology, College of Veterinary Medicine, Seoul National University) ;
  • Hong, Sung-Jong (Department of Medical Environmental Biology, Chung-Ang University College of Medicine)
  • Received : 2018.09.27
  • Accepted : 2018.10.19
  • Published : 2018.10.31
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Abstract

To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C ($1.0{\times}10^3$ or $1.0{\times}10^4$ tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

Keywords

References

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