Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Isolation and Characterization of an Anti-listerial Bacteriocin from Leuconostoc lactis SD501

  • Hwang, In-Chan (Department of Animal Resource Science, Dankook University) ;
  • Oh, Ju Kyoung (Department of Animal Resource Science, Dankook University) ;
  • Kim, Sang Hoon (Department of Animal Resource Science, Dankook University) ;
  • Oh, Sejong (Department of Animal Science, Chonnam National University) ;
  • Kang, Dae-Kyung (Department of Animal Resource Science, Dankook University)
  • Received : 2018.05.17
  • Accepted : 2018.08.09
  • Published : 2018.10.31
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Abstract

Although bacteriocins with anti-listerial activity have been isolated from a wide variety of lactic acid bacteria, little is known about those from Leuconostoc lactis, a heterofermentative bacterium that produces diacetyl and exopolysaccharides in dairy foods. In this study, an anti-listerial bacteriocin was isolated from Leuc. lactis SD501 and characterized. It was particularly potent against Listeria monocytogenes and also inhibited Enterococcus faecalis. Anti-listerial activity reached a maximum during the early stationary phase and then decreased gradually. The anti-listerial substance was sensitive to proteinase K and ${\alpha}$-chymotrypsin, confirming its proteinaceous nature. Its activity remained stable at pH values ranging from 1 to 10. In addition, it was strongly resistant to high temperatures, retaining its activity even after incubation for 15 min at $121^{\circ}C$. The apparent molecular mass of the partially purified anti-listerial bacteriocin was approximately 7 kDa. The characteristics of the SD501 bacteriocin, including its small molecular size (<10 kDa), strong anti-listerial activity, wide pH stability and good thermostability, indicate its classification as a Class IIa bacteriocin.

Keywords

References

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