대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제39권5호
  • /
  • Pages.188-207
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  • 2018
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  • 1229-0807(pISSN)
  • /
  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지
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Three-dimensional Bio-printing Technique: Trend and Potential for High Volume Implantable Tissue Generation

  • Duong, Van-Thuy (Department of Biomedical Engineering, University of Ulsan) ;
  • Kim, Jong Pal (Mobile Healthcare Laboratory, Samsung Advanced Institute Technology) ;
  • Kim, Kwangsoo (Department of Electronics and Control Engineering, Hanbat National University) ;
  • Ko, Hyoungho (Department of Electronics, Chungnam National University) ;
  • Hwang, Chang Ho (Department of Physical Medicine and Rehabilitation, Ulsan University Hospital, University of Ulsan College of Medicine) ;
  • Koo, Kyo-in (Department of Biomedical Engineering, University of Ulsan)
  • 투고 : 2018.06.01
  • 심사 : 2018.10.10
  • 발행 : 2018.10.31
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초록

Recently, three-dimensional (3D) printing of biological tissues and organ has become an attractive interdisciplinary research topic that combines a broad range of fields including engineering, biomaterials science, cell biology, physics, and medicine. The 3D bioprinting can be used to produce complex tissue engineering scaffolds based on computer designs obtained from patient-specific anatomical data. It is a powerful tool for building structures by printing cells together with matrix materials and biochemical factors in spatially predefined positions within confined 3D structures. In the field of the 3D bioprinting, three major categories of the 3D bioprinting include the stereolithography-based, inkjet-based, and dispensing-based bioprinting. Some of them have made significant process. Each technique has its own advantages and limitations. Compared with non-biological printing, the 3D bioprinting should consider additional complexities: biocompatibility, degradability of printing materials, cell types, cell growth, cell viability, and cell proliferation factors. Numerous 3D bioprinting technologies have been proposed, and some of them have been making great progress in printing several tissues including multilayered skin, cartilaginous structures, bone, vasculature even heart and liver. This review summarizes basic principles and key aspects of some frequently utilized printing technologies, and introduces current challenges, and prospects in the 3D bioprinting.

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참고문헌

  1. D. L. D. Bourell, J. J. Beaman, M. C. Leu, and D. W. Rosen, "A brief history of additive manufacturing and the 2009 roadmap for additive manufacturing: looking back and looking ahead," US-Turkey Work. …, no. 2, pp. 2005-2005, 2009.
  2. R.U.S.A. Data, P.E.H. Silbaugh, and A.E.L. Fertig, "Date of Patent : U.S. Patent," no. 19, 1990.
  3. M. Nakamura, S. Iwanaga, C. Henmi, K. Arai, and Y. Nishiyama, "Biomatrices and biomaterials for future developments of bioprinting and biofabrication," Biofabrication, vol. 2, no. 1, 2010.
  4. C.J. Ferris, K.G. Gilmore, G.G. Wallace, and M. In Het Panhuis, "Biofabrication: An overview of the approaches used for printing of living cells," Appl. Microbiol. Biotechnol., vol. 97, no. 10, pp. 4243-4258, 2013. https://doi.org/10.1007/s00253-013-4853-6
  5. M. Nakamura et al., "Biocompatible Inkjet Printing Technique for Designed Seeding of Individual Living Cells," Tissue Eng., vol. 11, no. 11-12, pp. 1658-1666, 2005. https://doi.org/10.1089/ten.2005.11.1658
  6. C.H. Droplets, S. Moon, D. Ph, S.K. Hasan, Y.S. Song, and D. Ph, "Layer by Layer Three-Dimensional Tissue," vol. 16, no. 1, 2010.
  7. S.V. Murphy and A. Atala, "3D bioprinting of tissues and organs," Nat. Biotechnol., vol. 32, no. 8, pp. 773-785, 2014. https://doi.org/10.1038/nbt.2958
  8. F. Pati et al., "Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink," Nat. Commun., vol. 5, pp. 1-11, 2014.
  9. L. Koch et al., "Laser printing of skin cells and human stem cells," Tissue Eng Part C Methods, vol. 16, no. 5, pp. 847-854, 2010. https://doi.org/10.1089/ten.tec.2009.0397
  10. M. Gruene et al., "Laser Printing of Stem Cells for Biofabrication of Scaffold-Free Autologous Grafts," Tissue Eng. Part C Methods, vol. 17, no. 1, pp. 79-87, 2011. https://doi.org/10.1089/ten.tec.2010.0359
  11. M. Gruene et al., "Laser Printing of Three-Dimensional Multicellular Arrays for Studies of Cell-Cell and Cell-Environment Interactions," Tissue Engineering Part C: Methods, vol. 17, no. 10. pp. 973-982, 2011. https://doi.org/10.1089/ten.tec.2011.0185
  12. and K. K. Ru Dai, Zongjie Wang, Roya Samanipour, Kyoin Koo, "Adipose-Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications," Stem Cells Int., vol. Volume 201, p. 19, 2016.
  13. J.A. Phillippi, E. Miller, L. Weiss, J. Huard, A. Waggoner, and P. Campbell, "Microenvironments Engineered by Inkjet Bioprinting Spatially Direct Adult Stem Cells Toward Muscle-and Bone-Like Subpopulations," Stem Cells, vol. 26, no. 1, pp. 127-134, 2008. https://doi.org/10.1634/stemcells.2007-0520
  14. E.D.F. Ker et al., "Engineering spatial control of multiple differentiation fates within a stem cell population," Biomaterials, vol. 32, no. 13, pp. 3413-3422, 2011. https://doi.org/10.1016/j.biomaterials.2011.01.036
  15. T. Xu, J. Jin, C. Gregory, J. J. Hickman, and T. Boland, "Inkjet printing of viable mammalian cells," Biomaterials, vol. 26, no. 1, pp. 93-99, 2005. https://doi.org/10.1016/j.biomaterials.2004.04.011
  16. T. Xu et al., "Viability and electrophysiology of neural cell structures generated by the inkjet printing method," Biomaterials, vol. 27, no. 19, pp. 3580-3588, 2006. https://doi.org/10.1016/j.biomaterials.2006.01.048
  17. Z. Wang, R. Abdulla, B. Parker, R. Samanipour, S. Ghosh, and K. Kim, "A simple and high-resolution stereolithography-based 3D bioprinting system using visible light crosslinkable bioinks," Biofabrication, vol. 7, no. 4, p. 45009, 2015. https://doi.org/10.1088/1758-5090/7/4/045009
  18. R. Chang, J. Nam, and W. Sun, "Effects of Dispensing Pressure and Nozzle Diameter on Cell Survival from Solid Freeform Fabrication-Based Direct Cell Writing," Tissue Engineering Part A, vol. 14, no. 1. pp. 41-48, 2008. https://doi.org/10.1089/ten.a.2007.0004
  19. R. Gauvin et al., "Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography," Biomaterials, vol. 33, no. 15, pp. 3824-3834, 2012. https://doi.org/10.1016/j.biomaterials.2012.01.048
  20. A. Tirella et al., "Substrate stiffness influences high resolution printing of living cells with an ink-jet system," J. Biosci. Bioeng., vol. 112, no. 1, pp. 79-85, 2011. https://doi.org/10.1016/j.jbiosc.2011.03.019
  21. E.A. Roth, T. Xu, M. Das, C. Gregory, J.J. Hickman, and T. Boland, "Inkjet printing for high-throughput cell patterning," Biomaterials, vol. 25, no. 17, pp. 3707-3715, 2004. https://doi.org/10.1016/j.biomaterials.2003.10.052
  22. Y.-J. Seol, T.-Y. Kang, and D.-W. Cho, "Solid freeform fabrication technology applied to tissue engineering with various biomaterials," Soft Matter, vol. 8, no. 6, pp. 1730-1735, 2012. https://doi.org/10.1039/C1SM06863F
  23. F.P. W. Melchels, J. Feijen, and D.W. Grijpma, "A review on stereolithography and its applications in biomedical engineering," Biomaterials, vol. 31, no. 24, pp. 6121-6130, 2010. https://doi.org/10.1016/j.biomaterials.2010.04.050
  24. R. Britain and M. Box, "Reseurch Britain," vol. 25, pp. 79-82, 1984.
  25. M.N. Cooke, J.P. Fisher, D. Dean, C. Rimnac, and A. G. Mikos, "Use of stereolithography to manufacture criticalsized 3D biodegradable scaffolds for bone ingrowth," J. Biomed. Mater. Res., vol. 64B, no. 2, pp. 65-69, 2003. https://doi.org/10.1002/jbm.b.10485
  26. K.W. Lee, S. Wang, B.C. Fox, E.L. Ritman, M.J. Yaszemski, and L. Lu, "Poly(propylene fumarate) bone tissue engineering scaffold fabrication using stereolithography: Effects of resin formulations and laser parameters," Biomacromolecules, vol. 8, no. 4, pp. 1077-1084, 2007. https://doi.org/10.1021/bm060834v
  27. F. Melchels, J. Malda, N. Fedorovich, J. Alblas, and T. Woodfield, Organ Printing. 2011.
  28. S. Maruo, "Development of Functional Devices Using Three-dimensional Micro / nano Stereolithography," vol. 3, no. 2, pp. 382-388, 2014.
  29. Y. Kajihara, T. Takeuchi, S. Takahashi, and K. Takamasu, "Development of a Nano-Stereolithography System Using Evanescent Light for Submicron Fabrication," Am. Soc. Precis. Eng. Annu. Meet., vol. 39, pp. 111-114, 2006.
  30. C. Sun, N. Fang, D.M. Wu, and X. Zhang, "Projection micro-stereolithography using digital micro-mirror dynamic mask," Sensors Actuators, A Phys., vol. 121, no. 1, pp. 113-120, 2005. https://doi.org/10.1016/j.sna.2004.12.011
  31. S. Maruo and K. Ikuta, "Submicron stereolithography for the production of freely movable mechanisms by using single-photon polymerization," Sensors Actuators, A Phys., vol. 100, no. 1, pp. 70-76, 2002. https://doi.org/10.1016/S0924-4247(02)00043-2
  32. J.S. Choi, H.W. Kang, I.H. Lee, T.J. Ko, and D.W. Cho, "Development of micro-stereolithography technology using a UV lamp and optical fiber," Int. J. Adv. Manuf. Technol., vol. 41, no. 3-4, pp. 281-286, 2009. https://doi.org/10.1007/s00170-008-1461-1
  33. F.P.W. Melchels, J. Feijen, and D. W. Grijpma, "A poly(d,llactide) resin for the preparation of tissue engineering scaffolds by stereolithography," Biomaterials, vol. 30, no. 23-24, pp. 3801-3809, 2009. https://doi.org/10.1016/j.biomaterials.2009.03.055
  34. C. Mandrycky, Z. Wang, K. Kim, and D. H. Kim, "3D bioprinting for engineering complex tissues," Biotechnol. Adv., vol. 34, no. 4, pp. 422-434, 2016. https://doi.org/10.1016/j.biotechadv.2015.12.011
  35. T.M. Seck, F.P.W. Melchels, J. Feijen, and D. W. Grijpma, "Designed biodegradable hydrogel structures prepared by stereolithography using poly(ethylene glycol)/poly(d,l-lactide)-based resins," J. Control. Release, vol. 148, no. 1, pp. 34-41, 2010. https://doi.org/10.1016/j.jconrel.2010.07.111
  36. F.P.W. Melchels, K. Bertoldi, R. Gabbrielli, A. H. Velders, J. Feijen, and D. W. Grijpma, "Mathematically defined tissue engineering scaffold architectures prepared by stereolithography," Biomaterials, vol. 31, no. 27, pp. 6909-6916, 2010. https://doi.org/10.1016/j.biomaterials.2010.05.068
  37. S.D. Gittard and R.J. Narayan, "Laser direct writing of micro- and nano-scale medical devices," Expert Rev Med Devices, vol. 7, no. 3, pp. 343-356, 2010. https://doi.org/10.1586/erd.10.14
  38. V. Chan, P. Zorlutuna, J. H. Jeong, H. Kong, and R. Bashir, "Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapsulation," Lab Chip, vol. 10, no. 16, p. 2062, 2010. https://doi.org/10.1039/c004285d
  39. K. Arcaute, B.K. Mann, and R.B. Wicker, "Stereolithography of three-dimensional bioactive poly(ethylene glycol) constructs with encapsulated cells," Ann. Biomed. Eng., vol. 34, no. 9, pp. 1429-1441, 2006. https://doi.org/10.1007/s10439-006-9156-y
  40. R. Raman et al., "High-Resolution Projection Microstereolithography for Patterning of Neovasculature," Adv. Healthc. Mater., vol. 5, no. 5, pp. 610-619, 2016. https://doi.org/10.1002/adhm.201500721
  41. T.M. Valentin et al., "Stereolithographic Printing of Ionically-Crosslinked Alginate Hydrogels for Degradable Biomaterials and Microfluidics," Lab Chip, 2017.
  42. R. Zhang and N. B. Larsen, "Stereolithographic hydrogel printing of 3D culture chips with biofunctionalized complex 3D perfusion networks," Lab Chip, 2017.
  43. C. Processing, "United States Patent," vol. 1, no. 12, 2003.
  44. R. G. Sweet, "High frequency recording with electrostatically deflected ink jets," Rev. Sci. Instrum., vol. 36, no. 2, pp. 131-136, 1965. https://doi.org/10.1063/1.1719502
  45. T. Wang, R. Patel, and B. Derby, "Manufacture of 3-dimensional objects by reactive inkjet printing," Soft Matter, vol. 4, no. 12, p. 2513, 2008. https://doi.org/10.1039/b807758d
  46. E. Sachs, M. Cima, and J. Cornie, "Three-dimensional printing: rapid tooling and prototypes directly form a CAD model," CIRP Ann. -Manuf. Technol., vol. 39, no. 1, pp. 201-204, 1990. https://doi.org/10.1016/S0007-8506(07)61035-X
  47. Y. Guo, H. S. Patanwala, B. Bognet, and A. W. K. Ma, "Inkjet and inkjet-based 3D printing: connecting fluid properties and printing performance," Rapid Prototyp. J., vol. 23, no. 3, pp. 562-576, 2017. https://doi.org/10.1108/RPJ-05-2016-0076
  48. R.A. Barry, R.F. Shepherd, J.N. Hanson, R.G. Nuzzo, P. Wiltzius, and J. A. Lewis, "Direct-write assembly of 3D hydrogel scaffolds for guided cell growth," Adv. Mater., vol. 21, no. 23, pp. 2407-2410, 2009. https://doi.org/10.1002/adma.200803702
  49. A. Seidi, M. Ramalingam, I. Elloumi-Hannachi, S. Ostrovidov, and A. Khademhosseini, "Gradient biomaterials for soft-to-hard interface tissue engineering," Acta Biomater., vol. 7, no. 4, pp. 1441-1451, 2011. https://doi.org/10.1016/j.actbio.2011.01.011
  50. H. Sirringhaus et al., "High-resolution Inkjet Printing of All- Transistor Circuits," Science (80-. )., vol. 290, no. 2000, pp. 2123-2126, 2000. https://doi.org/10.1126/science.290.5499.2123
  51. T. Shimoda, K. Morii, S. Seki, and H. Kiguchi, "Inkjet Printing of Light-Emitting Polymer Displays," MRS Bull., vol. 28, no. 11, pp. 821-827, 2003. https://doi.org/10.1557/mrs2003.231
  52. J. Bharathan and L. Angeles, "the ink-jet printing technology," vol. 3279.
  53. K. Crowley, E. O'Malley, A. Morrin, M. R. Smyth, and A. J. Killard, "An aqueous ammonia sensor based on an inkjetprinted polyaniline nanoparticle-modified electrode," Analyst, vol. 133, no. 3, p. 391, 2008. https://doi.org/10.1039/b716154a
  54. H.-Y. Chen et al., "Polymer solar cells with enhanced opencircuit voltage and efficiency," Nat. Photonics, vol. 3, no. 11, pp. 649-653, 2009. https://doi.org/10.1038/nphoton.2009.192
  55. A.M.J. van den Berg, P.J. Smith, J. Perelaer, W. Schrof, S. Koltzenburg, and U. S. Schubert, "Inkjet printing of polyurethane colloidal suspensions," Soft Matter, vol. 3, no. 2, pp. 238-243, 2007. https://doi.org/10.1039/B610017A
  56. A. Rida, L. Yang, R. Vyas, and M. M. Tentzeris, "Conductive inkjet-printed antennas on flexible low-cost paperbased substrates for RFID and WSN applications," IEEE Antennas Propag. Mag., vol. 51, no. 3, pp. 13-23, 2009. https://doi.org/10.1109/MAP.2009.5251188
  57. J. Perelaer, B.J. De Gans, and U.S. Schubert, "Ink-jet printing and microwave sintering of conductive silver tracks," Adv. Mater., vol. 18, no. 16, pp. 2101-2104, 2006. https://doi.org/10.1002/adma.200502422
  58. J. Vaithilingam et al., "3-Dimensional inkjet printing of macro structures from silver nanoparticles," Mater. Des., vol. 139, pp. 81-88, 2018. https://doi.org/10.1016/j.matdes.2017.10.070
  59. K.A.M. Seerden, N. Reis, J.R.G. Evans, P.S. Grant, J.W. Halloran, and B. Derby, "Ink-Jet Printing of Wax-Based Alumina Suspensions," J. Am. Ceram. Soc., vol. 84, no. 11, pp. 2514-2520, 2001. https://doi.org/10.1111/j.1151-2916.2001.tb01045.x
  60. B. Cappi, E. Ozkol, J. Ebert, and R. Telle, "Direct inkjet printing of Si3N4: Characterization of ink, green bodies and microstructure," J. Eur. Ceram. Soc., vol. 28, no. 13, pp.2625-2628, 2008. https://doi.org/10.1016/j.jeurceramsoc.2008.03.004
  61. R.J. Klebe, "Cytoscribing: A method for micropositioning cells and the construction of two- and three-dimensional synthetic tissues," Exp. Cell Res., vol. 179, no. 2, pp. 362-373, 1988. https://doi.org/10.1016/0014-4827(88)90275-3
  62. N.E. Sanjana and S.B. Fuller, "A fast flexible ink-jet printing method for patterning dissociated neurons in culture," J. Neurosci. Methods, vol. 136, no. 2, pp. 151-163, 2004. https://doi.org/10.1016/j.jneumeth.2004.01.011
  63. B. Derby, "Bioprinting: inkjet printing proteins and hybrid cell-containing materials and structures," J. Mater. Chem., vol. 18, no. 47, p. 5717, 2008. https://doi.org/10.1039/b807560c
  64. T. Okamoto, T. Suzuki, and N. Yamamoto, "Microarray fabrication with covalent attachment of DNA using Bubble Jet technology," Nat. Biotechnol., vol. 18, no. 4, pp. 438-441, 2000. https://doi.org/10.1038/74507
  65. J.T. Delaney, P.J. Smith, and U.S. Schubert, "Inkjet printing of proteins," Soft Matter, vol. 5, no. 24, p. 4866, 2009. https://doi.org/10.1039/b909878j
  66. B. Lorber, W.K. Hsiao, I.M. Hutchings, and K.R. Martin, "Adult rat retinal ganglion cells and glia can be printed by piezoelectric inkjet printing," Biofabrication, vol. 6, no. 1, 2014.
  67. B. Derby, "Additive Manufacture of Ceramics Components by Inkjet Printing," Engineering, vol. 1, no. 1, pp. 113-123, 2015. https://doi.org/10.15302/J-ENG-2015014
  68. S.B. Hong, N. Eliaz, E.M. Sachs, S.M. Allen, and R.M. Latanision, "Corrosion behavior of advanced titaniumbased alloys made by three-dimensional printing (3DPTM) for biomedical applications," Corros. Sci., vol. 43, no. 9, pp.1781-1791, 2001. https://doi.org/10.1016/S0010-938X(00)00181-5
  69. R. Noguera, M. Lejeune, and T. Chartier, "3D fine scale ceramic components formed by ink-jet prototyping process," J. Eur. Ceram. Soc., vol. 25, no. 12 SPEC. ISS., pp.2055-2059, 2005. https://doi.org/10.1016/j.jeurceramsoc.2005.03.223
  70. K.K.B. Hon, L. Li, and I.M. Hutchings, "Direct writing technology-Advances and developments," CIRP Annals-Manufacturing Technology, vol. 57, no. 2. pp. 601-620, 2008. https://doi.org/10.1016/j.cirp.2008.09.006
  71. N. Reis, C. Ainsley, and B. Derby, "Ink-jet delivery of particle suspensions by piezoelectric droplet ejectors," J. Appl. Phys., vol. 97, no. 9, 2005.
  72. T. Boland et al., "Drop-on-demand printing of cells and materials for designer tissue constructs," Mater. Sci. Eng. C, vol. 27, no. 3, pp. 372-376, 2007. https://doi.org/10.1016/j.msec.2006.05.047
  73. Y. Nishiyama et al., "Development of a Three-Dimensional Bioprinter: Construction of Cell Supporting Structures Using Hydrogel and State-Of-The-Art Inkjet Technology," J. Biomech. Eng., vol. 131, no. 3, p. 035001, 2009. https://doi.org/10.1115/1.3002759
  74. A. Butscher, M. Bohner, S. Hofmann, L. Gauckler, and R. Muller, "Structural and material approaches to bone tissue engineering in powder-based three-dimensional printing," Acta Biomater., vol. 7, no. 3, pp. 907-920, 2011. https://doi.org/10.1016/j.actbio.2010.09.039
  75. F.D. Modeling, "Rapid Prototyping Using FDM : A Fast , , Precise , Safe Technology," System, pp. 301-308, 1992.
  76. S. Knowlton, S. Onal, C.H. Yu, J.J. Zhao, and S. Tasoglu, "Bioprinting for cancer research," Trends Biotechnol., vol. 33, no. 9, pp. 504-513, 2015. https://doi.org/10.1016/j.tibtech.2015.06.007
  77. S. Khalil and W. Sun, "Biopolymer deposition for freeform fabrication of hydrogel tissue constructs," Mater. Sci. Eng. C, vol. 27, no. 3, pp. 469-478, 2007. https://doi.org/10.1016/j.msec.2006.05.023
  78. I.S. Scott Crump (Stratasys, "Apparatus and method for creating three-dimensional objects," vol. 2, no. 12, pp. 2-6, 1992.
  79. D.W. Hutmacher, T. Schantz, I. Zein, K.W. Ng, S.H. Teoh, and K.C. Tan, "Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling," J. Biomed. Mater. Res., vol. 55, no. 2, pp. 203-216, 2001. https://doi.org/10.1002/1097-4636(200105)55:2<203::AID-JBM1007>3.0.CO;2-7
  80. I. Zein, D. W. Hutmacher, K.C. Tan, and S.H. Teoh, "Fused deposition modeling of novel scaffold architectures for tissue engineering applications," Biomaterials, vol. 23, no. 4, pp. 1169-1185, 2002. https://doi.org/10.1016/S0142-9612(01)00232-0
  81. K. Jakab, C. Norotte, F. Marga, K. Murphy, G. Vunjak-Novakovic, and G. Forgacs, "Tissue engineering by selfassembly and bio-printing of living cells," Biofabrication, vol. 2, no. 2, 2010.
  82. F. Dolati, Y. Yu, Y. Zhang, A.M. De Jesus, E.A. Sander, and I. T. Ozbolat, "In vitro evaluation of carbon-nanotube-reinforced bioprintable vascular conduits," Nanotechnology, vol. 25, no. 14, 2014.
  83. V. Mironov, V. Kasyanov, and R.R. Markwald, "Nanotechnology in vascular tissue engineering: from nanoscaffolding towards rapid vessel biofabrication," Trends Biotechnol., vol. 26, no. 6, pp. 338-344, 2008. https://doi.org/10.1016/j.tibtech.2008.03.001
  84. D.B. Kolesky, R.L. Truby, A. S. Gladman, T.A. Busbee, K. A. Homan, and J. A. Lewis, "3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs," Adv. Mater., vol. 26, no. 19, pp. 3124-3130, 2014. https://doi.org/10.1002/adma.201305506
  85. "An additive manufacturing-based PCL-alginate chondroccyte bioprinted scaffold for cartilage tissue engineering.pdf.".
  86. J.S. Lee, J.M. Hong, J.W. Jung, J.H. Shim, J.H. Oh, and D. W. Cho, "3D printing of composite tissue with complex shape applied to ear regeneration," Biofabrication, vol. 6, no. 2, 2014.
  87. C. Norotte, F. S. Marga, L. E. Niklason, and G. Forgacs, "Scaffold-free vascular tissue engineering using bioprinting," Biomaterials, vol. 30, no. 30, pp. 5910-5917, 2009. https://doi.org/10.1016/j.biomaterials.2009.06.034
  88. R. Zhang and N.B. Larsen, "Stereolithographic hydrogel printing of 3D culture chips with biofunctionalized complex 3D perfusion networks," Lab Chip, 2017.
  89. B. Duan, L.A. Hockaday, K.H. Kang, and J.T. Butcher, "3D Bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels," J. Biomed. Mater. Res. -Part A, vol. 101 A, no. 5, pp. 1255-1264, 2013. https://doi.org/10.1002/jbm.a.34420
  90. V. Keriquel et al., "In vivo bioprinting for computer- and robotic-assisted medical intervention: Preliminary study in mice," Biofabrication, vol. 2, no. 1, 2010.
  91. L.D. Loozen, F. Wegman, F.C. Oner, W.J.A. Dhert, and J. Alblas, "Porous bioprinted constructs in BMP-2 non-viral gene therapy for bone tissue engineering," J. Mater. Chem. B, vol. 1, no. 48, p. 6619, 2013. https://doi.org/10.1039/c3tb21093f
  92. S. Catros et al., "Laser-assisted bioprinting for creating ondemand patterns of human osteoprogenitor cells and nanohydroxyapatite," Biofabrication, vol. 3, no. 2, 2011.
  93. X. Cui, K. Breitenkamp, M.G. Finn, M. Lotz, and D. D. D'Lima, "Direct Human Cartilage Repair Using Three-Dimensional Bioprinting Technology," Tissue Eng. Part A, vol. 18, no. 11-12, pp. 1304-1312, 2012. https://doi.org/10.1089/ten.tea.2011.0543
  94. T. Xu et al., "Hybrid printing of mechanically and biologically improved constructs for cartilage tissue engineering applications," Biofabrication, vol. 5, no. 1, 2013.
  95. J. Visser et al., "Biofabrication of multi-material anatomically shaped tissue constructs," Biofabrication, vol. 5, no. 3, 2013.
  96. S.P. Grogan et al., "Acta Biomaterialia Digital micromirror device projection printing system for meniscus tissue engineering," Acta Biomater., vol. 9, no. 7, pp. 7218-7226, 2013. https://doi.org/10.1016/j.actbio.2013.03.020
  97. S. Michael et al., "Tissue Engineered Skin Substitutes Created by Laser- Assisted Bioprinting Form Skin-Like Structures in the Dorsal Skin Fold Chamber in Mice," vol. 8, no.3, 2013.
  98. C.M. Owens, F. Marga, G. Forgacs, and C. M. Heesch, "Biofabrication and testing of a fully cellular nerve graft," 2013.
  99. "A 3D bioprinted complex structure for engineering the muscle- tendon unit.pdf." .
  100. Y. Zhao, R. Yao, L. Ouyang, and H. Ding, "Three-dimensional printing of Hela cells for cervical tumor model in vitro," 2014.
  101. M. Gruene, "Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells," 2011.
  102. J.J. Song, J.P. Guyette, S.E. Gilpin, G. Gonzalez, J.P. Vacanti, and H. C. Ott, "Regeneration and experimental orthotopic transplantation of a bioengineered kidney," Nat. Med., vol. 19, no. 5, pp. 646-651, 2013. https://doi.org/10.1038/nm.3154
  103. H. Onoe et al., "Metre-long cell-laden microfibres exhibit tissue morphologies and functions," Nat. Mater., vol. 12, no. 6, pp. 584-590, 2013. https://doi.org/10.1038/nmat3606
  104. Y.C. Li, Y.S. Zhang, A. Akpek, S.R. Shin, and A. Khademhosseini, "4D bioprinting: The next-generation technology for biofabrication enabled by stimuli-responsive materials," Biofabrication, vol. 9, no. 1, 2017.
  105. J. An, C.K. Chua, and V. Mironov, "A Perspective on 4D Bioprinting," Int. J. Bioprinting, vol. 2, no. 0, pp. 3-5, 2016.
  106. J. O. Hardin, T.J. Ober, A. D. Valentine, and J.A. Lewis, "Microfluidic printheads for multimaterial 3D printing of viscoelastic inks," Advanced Materials, vol. 27, no. 21. pp.3279-3284, 2015. https://doi.org/10.1002/adma.201500222
  107. W. Liu et al., "Rapid Continuous Multimaterial Extrusion Bioprinting," Adv. Mater., vol. 29, no. 3, pp. 1-8, 2017.
  108. L. Serex, A. Bertsch, and P. Renaud, "Microfluidics: A new layer of control for extrusion-based 3D printing," Micromachines, vol. 9, no. 2, 2018.
  109. M. Nie, P. Mistry, J. Yang, and S. Takeuchi, "Microfluidic enabled rapid bioprinting of hydrogel $\mu$fiber based porous constructs," Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2017. pp. 589-591.
  110. Z. Wang, R. Samanipour, K. Koo, and K. Kim, "Development and Investigation of a Sweetness Sensor for Sugars - Effect of Lipids-," Sensors Mater., no. February 2016, p. 1, 2015.
  111. W. Jia et al., "Direct 3D bioprinting of perfusable vascular constructs using a blend bioink," Biomaterials, vol. 106, pp. 58-68, 2016. https://doi.org/10.1016/j.biomaterials.2016.07.038
  112. C.Y. Lee, C.L. Chang, Y.N. Wang, and L.M. Fu, "Microfluidic mixing: A review," Int. J. Mol. Sci., vol. 12, no. 5, pp. 3263-3287, 2011. https://doi.org/10.3390/ijms12053263
  113. Y.Z. Liu, B.J. Kim, and H.J. Sung, "Two-fluid mixing in a microchannel," Int. J. Heat Fluid Flow, vol. 25, no. 6, pp. 986-995, 2004. https://doi.org/10.1016/j.ijheatfluidflow.2004.03.006
  114. A. D. Stroock, "Chaotic Mixer for Microchannels," Science (80-. )., vol. 295, no. 5555, pp. 647-651, 2002. https://doi.org/10.1126/science.1066238
  115. N. S. G. K. Devaraju and M. A. Unger, "Pressure driven digital logic in PDMS based microfluidic devices fabricated by multilayer soft lithography," Lab Chip, vol. 12, no. 22, p.4809, 2012. https://doi.org/10.1039/c2lc21155f
  116. T. Braschler et al., "A virtual valve for smooth contamination-free flow switching," Lab Chip, vol. 7, no. 9, p. 1111, 2007. https://doi.org/10.1039/b708360b
  117. J.B. Knight, A. Vishwanath, J.P. Brody, and R.H. Austin, "Hydrodynamic focusing on a silicon chip: Mixing nanoliters in microseconds," Phys. Rev. Lett., vol. 80, no. 17, pp. 3863-3866, 1998. https://doi.org/10.1103/PhysRevLett.80.3863
  118. R. Aoki, M. Yamada, M. Yasuda, and M. Seki, "In-channel focusing of flowing microparticles utilizing hydrodynamic filtration," Microfluid. Nanofluidics, vol. 6, no. 4, pp. 571-576, 2009. https://doi.org/10.1007/s10404-008-0334-0
  119. X. Xuan, J. Zhu, and C. Church, "Particle focusing in microfluidic devices," Microfluid. Nanofluidics, vol. 9, no. 1, pp. 1-16, 2010. https://doi.org/10.1007/s10404-010-0602-7
  120. A. Terray and S.J. Hart, "'Off-the-shelf' 3-D microfluidic nozzle," Lab Chip, vol. 10, no. 13, p. 1729, 2010. https://doi.org/10.1039/b927244e
  121. R.P. Visconti, V. Kasyanov, C. Gentile, J. Zhang, R. R. Markwald, and V. Mironov, "Towards organ printing: engineering an intra-organ branched vascular tree," Expert Opin. Biol. Ther., vol. 10, no. 3, pp. 409-420, 2010. https://doi.org/10.1517/14712590903563352
  122. J.M. Perez-Pomares, V. Mironov, J.A. Guadix, D. Macias, R. R. Markwald, and R. Munoz-Chapuli, "In vitro selfassembly of proepicardial cell aggregates: An embryonic vasculogenic model for vascular tissue engineering," Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, vol. 288, no. 7. pp. 700-713, 2006.
  123. J. Kreuter, "Nanoparticles and microparticles for drug and vaccine delivery.," J. Anat., vol. 189 (Pt 3, no. Ii, pp. 503-505, 1996.
  124. V.T. Duong et al., "Twenty-Day Culturing of Tubular Scaffolds Using Micro- Connector With Heart-Mimicking Medium Pumping for Blood Vessel Modeling," MicroTAS 2017, 2017.
  125. D. Oh, S. Lee, K. Koo, and J. Seo, "6th European Conference of the International Federation for Medical and Biological Engineering," vol. 45, pp. 322-325, 2015.
  126. J.S. Miller et al., "Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues," Nat. Mater., vol. 11, no. 9, pp. 768-774, 2012. https://doi.org/10.1038/nmat3357
  127. T.J. Hinton et al., "Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended... Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels," no. Oct., 2015.