Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
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Detection of Sugar Beet (Beta vulgaris) - Specific Gene from Honey Made by Sugar of Sugar Beet

사탕무(Beta vulgaris) 설탕으로 제조된 사양꿀에서 사탕무 고유 유전자의 검출

  • Kim, Somin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Byounghee (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Moonjung (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Jungmin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Truong, A Tai (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Yoon, Byoungsu (Department of Life Science, College of Fusion Science, Kyonggi University)
  • 김소민 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • 김병희 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • 김문정 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • 김정민 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • ;
  • 윤병수 (경기대학교 융합과학대학 바이오융합학부 생명과학전공)
  • Received : 2018.08.31
  • Accepted : 2018.09.21
  • Published : 2018.09.30
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Abstract

We could detect the specific gene of sugar beet (Beta vulgaris) from honey produced by sugar of sugar beet using the ultra-rapid real-time PCR (UR-qPCR). In our knowledge, it is the first report in the world that PCR detection of sugar beet-specific gene from adulterated honey. Through extracting the DNA (using CTAB method) from sugar and honey of sugar beet and using the UR-qPCR, sugar beet-specific DNA sequence could be amplified quantitatively until $10^0$ molecules of initial template. By using nested PCR and DNA sequencing, its specificity was confirmed. DNA sequence was matched 100% with mitochondria gene of sugar beet. This finding that trace DNA in adulterated honey could be genetically analysed, would be used as a decisive evidence for the authenticity test of honey.

Keywords

Acknowledgement

Supported by : 농촌진흥청, 농림수산식품기술기획평가원, 중소기업청

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