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Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2

  • Lee, Su-Young (Department of Prosthodontics, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Koak, Jai-Young (Department of Prosthodontics and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Kim, Seong-Kyun (Department of Prosthodontics and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Heo, Seong-Joo (Department of Prosthodontics and Dental Research Institute, School of Dentistry, Seoul National University)
  • Received : 2018.04.17
  • Accepted : 2018.06.19
  • Published : 2018.10.01

Abstract

BACKGROUND: The purpose of this study is to examine physical characteristics of and initial biological properties to anodized titanium treated with poly($\text\tiny{D,L}$-lactide-co-glycolide) (PLG) mixed with recombinant human bone morphogenic protein-2 (rhBMP-2). METHODS: Titanium specimens were prepared in groups of four as follows: group NC was anodized under 300 V as control; group PC was anodized then dropped and dried with solution 0.02 ml PLG; group D was anodized then dropped and dried with solution 0.02 ml PLG/rhBMP-2 ($3.75{\mu}g$ per disc); and group E was anodized then coated with 0.02 ml PLG/rhBMP-2 ($3.75{\mu}g$ per disc) by electrospray. Human osteoblastic-like sarcoma cells were cultured. Cell proliferation and alkaline phosphatase (ALP) activity test were carried out. Runx-2 gene was investigated by the reverse transcription-polymerase chain reaction. Immunofluorescence outcome of osteogenic proteins was observed. RESULTS: After 3 days, there were significantly higher proliferations compared rhBMP-2 loaded titanium discs with rhBMP-2 unloaded discs. The ALPase activity on rhBMP-2 loaded titanium discs was significantly higher than in rhBMP-2 unloaded discs. The expression level of Runx2 mRNA presented the highest on the PLG/rhBMP-2-coated surface. CONCLUSION: PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium.

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