The Korean Journal of Blood Transfusion (대한수혈학회지)

The Korean Society of Blood Transfusion (대한수혈학회)
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Freezing and Washing of Red Blood Cells Using Haemonetics ACP 215

Haemonetics ACP 215를 이용한 적혈구의 동결과 세척

  • Youn, Kyoung Won (Blood Transfusion Research Institute, Korean Red Cross) ;
  • Choi, Kyoung Young (Jungbu Blood Laboratory Center, Korean Red Cross) ;
  • Lee, Sun Ah (Department of Laboratory Medicine, Myongji St. Mary's Hospital) ;
  • Min, Hyuk Ki (Blood Transfusion Research Institute, Korean Red Cross) ;
  • Kim, Jaehyun (Blood Transfusion Research Institute, Korean Red Cross)
  • 윤경원 (대한적십자사 혈액수혈연구원) ;
  • 최경영 (대한적십자사 중부혈액검사센터) ;
  • 이선아 (명지성모병원 진단검사의학과) ;
  • 민혁기 (대한적십자사 혈액수혈연구원) ;
  • 김재현 (대한적십자사 혈액수혈연구원)
  • Received : 2018.09.07
  • Accepted : 2018.10.30
  • Published : 2018.12.31
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Abstract

Background: The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim of this study was to assess glycerolization and deglycerolization processing for RBCs using a high glycerol method in the automated, closed system provided by Haemonetics ACP 215. Methods: Thirty-five packed RBCs were glycerolized using the ACP 215 to a final concentration of 40% (wt/vol). The units were either frozen as such (n=30) or excess glycerol was removed (n=5) before freezing. After storage at $-80^{\circ}C$, the units were thawed, deglycerolized and resuspended in SAG-M. The frozen-thawed RBCs were stored at $4^{\circ}C$, and analyzed for their stability and in vitro quality. Results: No prefreeze excess glycerol removal units showed significantly less potassium leakage during post-thaw storage compared to the prefreeze excess glycerol removal units. All measurements of the stability and in vitro quality of thawed RBCs prepared from frozen RBCs without the prefreeze removal of excess glycerol during post-thaw storage at $4^{\circ}C$ for 7 days were acceptable to the American Blood Bank Association's standards and European standards. Conclusion: RBCs frozen without prefreeze removal of excess glycerol and the ACP 215 simplifies cryopreservation procedure and increases the stability of frozen-thawed RBCs. This increases the practical applicability of cryopreserved RBCs in blood transfusion practice.

배경: 적혈구를 폐쇄계에서 동결하고 세척하는 경우 동결해동적혈구의 보존기간을 24시간 이상으로 연장할 수 있다. 본 연구에서는 고농도글리세롤법과 자동화 장비인 Haemonetics ACP 215를 이용한 폐쇄계에서 적혈구의 동결과 세척과정을 평가하였다. 방법: ACP 215를 이용하여 35단위의 적혈구에 글리세롤을 최종농도가 40% (wt/vol)가 되도록 첨가하고 30단위는 즉시 동결하였으며, 나머지 5단위는 잔여 글리세롤을 제거한 다음 동결하였다. 동결한 적혈구는 $-80^{\circ}C$에 보관 후 해동 세척하여 SAG-M 보존액에 부유하고, $4^{\circ}C$에서 보존하면서 안정성 및 품질관리 지표를 분석하였다. 결과: 잔여 글리세롤을 제거하지 않은 동결적 혈구는 잔여 글리세롤을 제거한 동결해동적혈구에 비해 해동 후 보존기간 동안 칼륨 유출이 현저히 낮게 나타났다. 잔여 글리세롤을 제거하지 않은 동결해동적혈구를 해동 후 $4^{\circ}C$에서 7일간 보존하는 동안 측정한 모든 안정성 및 품질지표는 AABB와 유럽연합의 기준에 모두 적합하였다. 결론: ACP 215를 사용하여 동결 전 잔여 글리세롤을 제거하지 않고 바로 적혈구를 동결하는 방법은 동결보존과정이 단순하고 동결해동적혈구의 안정성이 향상되어 향후 수혈을 위한 동결적혈구에 현실적으로 적용가능성이 높은 것으로 생각된다.

Keywords

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