Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Anti-obesity Activities of Cirsium setidens Nakai Ethanolic Extract

고려엉겅퀴 주정 추출물을 함유하는 임상시험제품의 항비만 활성 평가

  • Cho, Bong-Yeon (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Sun-Il (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Seung-Hyun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Sim, Wan-Sup (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Xionggao, Han (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Ra, Moon-Jin (Hongcheon Institute of Medicinal Herb) ;
  • Kim, Sun-Young (Hongcheon Institute of Medicinal Herb) ;
  • Kang, Il-Jun (Department of Food Science and Nutrition, Hallym University) ;
  • Han, Kyoung-Chan (HATTI Co., Ltd.) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • Received : 2018.05.14
  • Accepted : 2018.06.25
  • Published : 2018.10.30
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Abstract

The purpose of this study was to evaluate anti-obesity activity of Cirsium setidens Nakai test material (CNTM) in 3T3-L1 adipocytes and obese C57BL/6J mice fed with a high-fat diet using various obesity-related in vitro experiments. During adipocyte differentiation, CNTM significantly inhibited lipid accumulation and ROS production compared to controls. To evaluate whether CNTM could exert glycerol release effects on mature 3T3-L1 adipocytes, we treated cells with various concentrations of CNTM for 1 h. Treatment of mature adipocytes with $160-320{\mu}g/mL$ of CNTM increased the release of glycerol, but not in a significant dose-dependent manner. Anti-adipogenic and anti-lipogenic effects of CNTM seemed to be mediated by the inhibition of $PPAR{\gamma}$ and $C/EBP{\alpha}$. Moreover, CNTM stimulated fatty acid oxidation in an AMPK-dependent manner. CNTM-treated groups of C57BL/6J mice showed reduced body weights and adipose tissue weight with improving serum lipid profiles and adiponectin protein expression in obese C57BL/6J mice fed with a high-fat diet. These results suggest that CNTM might have anti-obesity effect on adipogenesis and lipid metabolism in vitro and in vivo. This presents the possibility of developing a treatment for obesity using nontoxic natural resources.

본 연구는 고려엉겅퀴 주정추출물을 가지고 제작된 임상시험용제품(CNTM)이 3T3-L1 지방세포 및 고지방식이로 유도된 비만 쥐에 미치는 항비만 효능 관찰을 통하여 체지방 개선 기능성식품을 개발하기위하여 인체적용시험 시료를 제작한 후, 인체적용시험전에 그 효력이 유지됨을 확인하기위해 수행되었다. 본 연구에 사용된 시료 CNTM은 $80{\sim}320{\mu}g/mL$ 농도에서 세포독성이 관찰되지 않았으며, 지방 축적억제 효능 및 지방세포 분화, 지질대사 관련 유전인자들을 유의적으로 변화시키는 것으로 확인되었다. 또한 동물실험에서 CNTM 처리에 의하여 체중의 감소를 확인하였으며 혈중지질 성분 가운데 HDL-C/TC의 비율은 유의적으로 증가하며 LDL-cholesterol (LDL-C)과 non-esterified fatty acid (NEFA)의 비율은 유의적으로 감소하여 혈중 유리지방산 농도를 감소시키며 혈중 콜레스테롤 상승을 억제시키고 혈당을 감소시키는 효과가 있는 것으로 평가되었다. 따라서 고려엉겅퀴 주정추출물이 함유된 인체적용시험시료(CNTM)는 체지방 개선에 우수한 효과가 있는 것으로 확인되었다.

Keywords

References

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