The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of an Extract of Fermented Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix on Anti-inflammation Associated with Dyslipidemia and Anti-oxidation in RAW264.7 and HUVEC Cells

한인진(韓茵蔯), 울금(鬱金), 산사(山査), 단삼(丹蔘) 발효복합추출물이 RAW264.7 및 HUVEC 세포에서 나타나는 이상지질혈증 관련 염증인자 발현 및 항산화에 미치는 영향

  • Lee, Kang-wook (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Cho, Hyun-kyoung (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Yoo, Ho-ryong (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Seol, In-chan (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Dae-Jeon University) ;
  • Kim, Yoon-sik (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Dae-Jeon University)
  • 이강욱 (대전대학교 한의과대학 심계내과학교실) ;
  • 조현경 (대전대학교 한의과대학 심계내과학교실) ;
  • 유호룡 (대전대학교 한의과대학 심계내과학교실) ;
  • 설인찬 (대전대학교 한의과대학 심계내과학교실) ;
  • 김윤식 (대전대학교 한의과대학 심계내과학교실)
  • Received : 2018.07.30
  • Accepted : 2018.09.28
  • Published : 2018.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: To investigate the effect of fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix (FMH) on anti-inflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC cells. Methods: The total polyphenols, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity, and cytotoxicity of FMH were measured. RAW264.7 cells treated with FMH were tested for production of NO, and for cytokine and LTB4 levels and HUVEC cells treated with FMH were examined for production of cDNA of genes related to inflammation. Results: 1. FMH contained polyphenols and flavonoids. The DPPH and ABTS radical scavenging activity of FMH increased in a concentration-dependent manner. 2. FMH treatment inhibited the production of nitric oxide (NO), cytokines, and LTB4 in RAW264.7 cell when compared to the untreated control group. 3. FMH decreased the transcription of pro-inflammatory genes, whereas it increased transcription of anti-inflammatory genes, in HUVEC cells. Conclusion: FMH is effective as an antioxidant and for treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke, and other cardiocerebrovascular diseases.

Keywords

References

  1. Statistics Korea. 2016 Death statistics. Korea: Korea development institute: 2016.
  2. Korean Academy of Medical Sciences & Korea Centers for Disease Control and Prevention. Guideline for Dyslipidemia in Primary Care. Seoul: Guideline Information Center; 2016.
  3. National Oriental Medical Department of Heart Internal Medicine. Oriental neurocirculatory Internal Medicine. 3rd edition. Seoul: Gunja publishing company; 2011. p. 184-5.
  4. Cha BS. Step to Internal Medicine Endocrinology. 4th edition. Seoul: Jungdam publishing company; 2018, p. 389.
  5. Cho SY, Choi HJ, Choi JW, Ha WHo Lee SJ. Antiobesity and Antihyperlipidemia of Red Crab Shells in Sprague Dawley Rat. J Chjtjn Chjtosan 2010 ;15(3):156-63.
  6. An JJ, Cho HK, Kim YS, Park SA, Seol IC, Yoo HR. The Effects of Gamijihwang-tang(GJT) on Hyperlipidemia in Rats. J Int Korean Med 2009;30(2):338-54.
  7. Lee WC, Oh KH. Nuroprotective Effect Yanggyuksanhwatang on Cerebral Infarction Induced by MCAO in Hyperlipidemic Rats. J Int Korean Med 2006; 27(4):915-26.
  8. Choi SH, Kim JM, Seol IC, Son CG. Study for Trends of Antihyperlipidemic Studies using Herbal Plants. J Int Korean Med 2010;31(3):448-56.
  9. Han JM, Im HJ, Jo HK, Lee JS, Lee SJ, Son CG, et al. ACE Reduces Metabolic Abnormalities in a High-Fat Diet Mouse Model. eCAM 2015;2015(352647):8.
  10. Kim HT, Kim YS, Seol IC, Yoo HR. Effects of an Extract of Artemisiae Capillaris, Curcumae Longae, and Crataegi Fructus (IUS) on Antihyperlipidemia and Anti-oxidation in db/db Mouse Model. J Int Korean Med 2016;37(3):467-83.
  11. Cho HK, Kim YS, Ryu JY, Seol IC, Yoo HR. The Effects of Artemisiae Iwayomogii Herba, Curcumae Radix, and Aurantii Fructus Immaturus Complex Extract (ACA) on Dyslipidemia-related Factor Expression and Anti-oxidation in HepG2 Cells. J Int Korean Med 2017;38(3):367-75. https://doi.org/10.22246/jikm.2017.38.3.367
  12. Cha JY, Cho HK, Kim YS, Seol IC, Yoo HR. Inhibition of gene associated with Dyslipidemia and Antioxidative Effect of Artemisia iwayomogi, Curcumae Radix and Raphani Semen(ACR) on HepG2 cell model. J Korean Med 2017;38(3):43-58. https://doi.org/10.13048/jkm.17024
  13. Cho HK. Jung ES, Kim YS, Seol I C, Yoo HR. Antioxidant and Anti-dyslipidemic Effect of Artemisiae iwayomogii Herba, Curcumae longae Radix, and Plantaginis Semen Complex Extract(ACP) on HepG2 Cells. J Physiol & Pathol Korean Med 2018;32(1):13-23. https://doi.org/10.15188/kjopp.2018.02.32.1.13
  14. Lee CS, Woo MS. progress in oriental medicine clinical treatment of common diseases. 1st edition. Hanam: China chinese medicine publishing company; 1991, p. 81.
  15. Textbook Compilation Committee of Oriental pathology. Oriental pathology. 2nd edition. Seoul: Oriental medicine cultural company; 2008, p. 94, 113.
  16. Kim HJ, Kwon OJ, Lee AR. Roh SS, Seo YB. Protective effects of Gastrodia rhizoma and steamed & fermented Gastrodiae rhizoma with anti-oxidant efficacy and suppression of NFKB signa ling pathway on LPS-induced liver injury. J Appl BioI Chem 2016;59(3):179-88. https://doi.org/10.3839/jabc.2016.033
  17. Feng XJ, Oh DS, Roh SS, Seo YB. Lipid Improvement Effect of Fermented Cynanchi wilfordii Radix in Hyperlipidemia Rats: Erratum. Kor J Herbology 2015;30(6):83-91. https://doi.org/10.6116/kjh.2015.30.6.83.
  18. Kang DG. Effects of the Fermented Extract Using the Leaves of Pinus densiflora on Hyperlipidemia Rats. J Physiol & Pathol Korean Med 2003;17(1):140-5.
  19. Cho HK. Choi KE, Kim YS, Seol IC, Yoo HR. Hypolipidemic and Anti-oxidant Effects of Chunghyl Plus in Type IT Diabetic Mice Model. J Physiol & Pathol Korean Med 2016;30(3): 164-76. https://doi.org/10.15188/kjopp.2016.06.30.3.164
  20. World Health Organization. Global Health Estimates 2016: Deaths by Cause, Age, Sex, by Country and by Region, 2000-2016. Geneva: WHO: 2018.
  21. Committee for Guidelines for Management of Dyslipidemia. 2015 Korean Guidelines for Management of Dyslipidemia. J Lipid AtheroscJer 2015;4(1):61-92. https://doi.org/10.12997/jla.2015.4.1.61
  22. Yang YG. The translation of Hwangjenaegyung. 1st edition. Seoul: Iljoongsa ; 1991, p. 296-7, 416.
  23. Bang HJ, Hong y, Kang YH, Tak US. The Oriental Medicine Review of Dyslipidemia. The journal of east-west medicines 1995;20(1):25-36.
  24. Han HS. Effect of Fermented Artemisiae Argyi Folium on Human Hepatoma Cell Line HepG2 Activity. Kor J Herbology 2013;28(3):107-13. https://doi.org/10.6116/kjh.2013.28.3.107
  25. Herbal medicine Compilation Committee of College of Oriental Medicine. Herbal medicine. 2nd edition. Seoul: Younglimsa; 2010, p. 369, 411-2, 453, 458.
  26. Go BS. The encyclopedia of Chinese Herbal medicine. 2nd edition. Daebukseong: Shinmoonpung; 1974, p. 216, 303-4, 1604, 2639.
  27. Hao G, Le GW, Li W, Shi YH, Yang RL. Increasing Oxidative Stress with Progressive Hyperlipidemia in Human: Relation between Malondialdehyde and Atherogenic Index. J Clin Biochem Nutr 2008;4(3):154-58.
  28. Barber RD, Clark BJ, Coleman RA, Harmer DW. GAPD H as a housekeeping gene: analysis of GAPD H mRNA expression in a panel of 72 human tissues. Physiol Genomics 2005;21(3): 389-484. https://doi.org/10.1152/physiolgenomics.00025.2005
  29. Christopherson K. Hromas R. Chemokine Regulation of Normal and Pathologic Immune Responses. Stem Cells 2001:19(5):388-96. https://doi.org/10.1634/stemcells.19-5-388
  30. Harrison's Internal Medicine Editorial Board of the Korean Society of Internal Medicine. Harrison's Principles of Internal Medicine. 15th edition. Seoul: MIP; 2003, p. 1420.

Cited by

  1. FDY003의 항산화활성 및 표준화 연구 vol.40, pp.6, 2019, https://doi.org/10.22246/jikm.2019.40.6.1112
  2. 해백청혈플러스(AMCP)의 항산화 및 항염증 작용을 통한 죽상동맥경화 억제 효과 vol.34, pp.3, 2020, https://doi.org/10.15188/kjopp.2020.06.34.3.126
  3. 유산균으로 발효한 침향공진단으로부터 분리한 Nodakenetin의 Neuraminidase 활성 억제 효능 vol.48, pp.3, 2018, https://doi.org/10.4014/mbl.2001.01015