Research in Vestibular Science

The Korean Balance Society (대한평형의학회)
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Application of Tetrode Technology for Analysis of Changes in Neural Excitability of Medial Vestibular Nucleus by Acute Arterial Hypotension

급성저혈압에 의한 내측전정신경핵 신경세포의 흥분성 변화를 분석하기 위한 테트로드 기법의 적용

  • Kim, Young (Department of Emergency Medicine, Wonkwang University School of Medicine & Hospital) ;
  • Koo, Ho (Institute of Brain Science, Wonkwang University School of Medicine) ;
  • Park, Byung Rim (Department of Physiology, Wonkwang University School of Medicine) ;
  • Moon, Se Jin (Department of Physiology, Wonkwang University School of Medicine) ;
  • Yang, Seung-Bum (Department of Medical Non-commissioned Officer, Wonkwang Health Science University) ;
  • Kim, Min Sun (Department of Physiology, Wonkwang University School of Medicine)
  • 김영 (원광대학교 의과대학 부속병원 응급의학교실) ;
  • 구호 (원광대학교 의과대학 뇌과학연구소) ;
  • 박병림 (원광대학교 의과대학 생리학교실) ;
  • 문세진 (원광대학교 의과대학 생리학교실) ;
  • 양승범 (원광보건대학교 의무부사관과) ;
  • 김민선 (원광대학교 의과대학 생리학교실)
  • Received : 2018.09.16
  • Accepted : 2018.10.24
  • Published : 2018.12.15
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Abstract

Objectives: Excitability o medial vestibular nucleus (MVN) in the brainstem can be affected by changes in the arterial blood pressure. Several animal studies have demonstrated that acute hypotension results in the alteration of multiunit activities and expression of cFos protein in the MVN. In the field of extracellular electrophysiological recording, tetrode technology and spike sorting algorithms can easily identify single unit activity from multiunit activities in the brain. However, detailed properties of electrophysiological changes in single unit of the MVN during acute hypotension have been unknown. Methods: Therefore, we applied tetrode techniques and electrophysiological characterization methods to know the effect of acute hypotension on single unit activities of the MVN of rats. Results: Two or 3 types of unit could be classified according to the morphology of spikes and firing properties of neurons. Acute hypotension elicited 4 types of changes in spontaneous firing of single unit in the MVN. Most of these neurons showed excitatory responses for about within 1 minute after the induction of acute hypotension and then returned to the baseline activity 10 minutes after the injection of sodium nitroprusside. There was also gradual increase in spontaneous firing in some units. In contrast small proportion of units showed rapid reduction of firing rate just after acute hypotension. Conclusions: Therefore, application of tetrode technology and spike sorting algorithms is another method for the monitoring of electrical activity of vestibular nuclear during acute hypotension.

Keywords

Acknowledgement

Supported by : 원광대학교

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