Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

New Report of Three Unrecorded Species in Trichoderma harzianum Species Complex in Korea

  • Jang, Seokyoon (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Kwon, Sun Lul (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Lee, Hanbyul (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University) ;
  • Jang, Yeongseon (Division of Wood Chemistry and Microbiology, National Institute of Forest Science) ;
  • Park, Myung Soo (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Lim, Young Woon (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Kim, Changmu (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Kim, Jae-Jin (Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University)
  • Received : 2017.11.07
  • Accepted : 2018.05.05
  • Published : 2018.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The genus Trichoderma (Hypocreaceae, Ascomycota) consists of globally distributed fungi. Among them, T. harzianum, one of the most commonly collected Trichoderma species, had been known as a polyphyletic or aggregate species. However, a total of 19 species were determined from the polyphyletic groups of T. harzianum. Thus, we explored Korean "T. harzianum" specimens that were collected in 2013-2014. These specimens were re-examined based on a recent study with translate elongation factor 1-alpha ($EF1{\alpha}$) sequences to reveal cryptic Trichoderma species in Korea. As a result, four different species, T. afroharzianum, T. atrobruneum, T. pyramidale, and T. harzianum, were identified. Except T. harzianum, the other three species have not been reported in Korea. In this work, we describe these species and provide figures.

Keywords

References

  1. Bissett J, Gams W, Jaklitsch W, et al. Accepted Trichoderma names in the year 2015. IMA Fungus. 2015;6:263-295. https://doi.org/10.5598/imafungus.2015.06.02.02
  2. Druzhinina I, Seidl-Seiboth V, Herrera-Estrella A, et al. Trichoderma: the genomics of opportunistic success. Nat Rev Micro. 2011;9:749-759. https://doi.org/10.1038/nrmicro2637
  3. Druzhinina I, Kubicek CP. Species concepts and biodiversity in Trichoderma and Hypocrea: from aggregate species to species clusters?. J Zhejiang Univ SciB. 2005;6:100-112. https://doi.org/10.1631/jzus.2005.AS0100
  4. Chaverri P, Branco-Rocha F, Jaklitsch W, et al. Systematics of the Trichoderma harzianum species complex and the re-identification of commercial biocontrol strains. Mycologia. 2015;107:558-590. https://doi.org/10.3852/14-147
  5. Samson RA, Hoekstra ES, Frisvad JC, et al. Introduction to food-and airborne fungi. 6th ed. Utrecht: Centraalbureau voor Schimmelcultures (CBS); 2002.
  6. Gams W, Meyer W. What exactly is Trichoderma harzianum? Mycologia. 1998;90:904-915. https://doi.org/10.1080/00275514.1998.12026984
  7. Lee S, Hong SB, Kim CY. Contribution to the checklist of soil-inhabiting fungi in Korea. Mycobiology. 2003;31:9-18. https://doi.org/10.4489/MYCO.2003.31.1.009
  8. Park M, Seo GS, Bae KS, et al. Characterization of Trichoderma spp. associated with green mold of oyster mushroom by PCR-RFLP and sequence analysis of ITS regions of rDNA. Plant Pathol J. 2005;21:229-236. https://doi.org/10.5423/PPJ.2005.21.3.229
  9. Huh N, Jang Y, Lee J, et al. Phylogenetic analysis of major molds inhabiting woods and their discoloration characteristics. Part 1. Genus Trichoderma. Holzforschung. 2011;65:257-263.
  10. Lee H, Lee YM, Heo YM, et al. Optimization of endoglucanase production by Trichoderma harzianum KUC1716 and enzymatic hydrolysis of lignocellulosic biomass. Bioresources. 2015;10:7466-7476.
  11. Jaklitsch WM. European species of Hypocrea Part I. The green-spored species. Stud Mycol. 2009;63:1-91. https://doi.org/10.3114/sim.2009.63.01
  12. Lee HK, Lee BH. Taxonomical studies of genus Trichoderma in Korea. Kor J Microbiol. 1980;18:73-90.
  13. Jang S, Jang Y, Kim CW, et al. Five new records of soil-derived Trichoderma in Korea: T. albolutescens, T. asperelloides, T. orientale, T. spirale, and T. tomentosum. Mycobiology. 2017;45:1-8. https://doi.org/10.5941/MYCO.2017.45.1.1
  14. Samuels GJ, Dodd SL, Gams W, et al. Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia. 2002;94:146-170. https://doi.org/10.1080/15572536.2003.11833257
  15. Munsell color. Munsell soil color charts with genuine Munsell color chips. Michigan, USA: Grand Rapids; 2009
  16. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous Ascomycetes. Mycologia. 1999;91:553-556. https://doi.org/10.2307/3761358
  17. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30:772-780. https://doi.org/10.1093/molbev/mst010
  18. Maddison D, Maddison W. MacClade 4: analysis of phylogeny and character evolution. Version 4.08. Sunderland, MA: Sinauer Associates, 2005.
  19. Nylander JAA. MrModeltest v2. Program distributed by the author. Uppsala, Sweden: Evolutionary biology centre. Uppsala University, 2004.
  20. Ronquist F, Huelsenbeck JP. MRBAYES 3: bayesian phylogenetic inference under mixed models. Bioinformatics. 2003;19:1572-1574. https://doi.org/10.1093/bioinformatics/btg180
  21. Hong JH, Jang S, Heo YM, et al. Investigation of marine-derived fungal diversity and their exploitable biological activities. Mar Drugs. 2015;13:4137-4155. https://doi.org/10.3390/md13074137

Cited by

  1. Endophytic Trichoderma Species Isolated from Persea americana and Cinnamomum verum Roots Reduce Symptoms Caused by Phytophthora cinnamomi in Avocado vol.9, pp.9, 2020, https://doi.org/10.3390/plants9091220