Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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The Effect Estrogen and Testosterone on the Expression of Melanogenesis-related Genes in Korean Brindle Cattle

칡소 모색별 피부세포 배양 시 Estrogen 과 Testosterone 첨가가 Melanogenesis 관련 유전자의 발현에 미치는 영향

  • Seo, Hui-Gyeong (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Lee, Ji-Hye (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Kim, Sang-Hwan (Institute of Genetic Engineering, Hankyong National University) ;
  • Lee, Ho-Jun (Institute of Genetic Engineering, Hankyong National University) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 서희경 (한경대학교 미래융합기술대학원 동물생명공학과) ;
  • 이지혜 (한경대학교 미래융합기술대학원 동물생명공학과) ;
  • 김상환 (한경대학교 유전공학 연구소) ;
  • 이호준 (한경대학교 유전공학 연구소) ;
  • 윤종택 (한경대학교 유전공학 연구소)
  • Received : 2018.06.08
  • Accepted : 2018.09.20
  • Published : 2018.09.30
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Abstract

The purpose of this study is to expression pattern of melanogenesis associate genes on cultured melanocyte layer cells in Korean Brindle Cattle(Dark, Brindle and Yellow) were analyzed to evaluate the effects of sex hormones on the control of melanogenesis pathways. Korean Brindle Cattle(Dark, Brindle and Yellow) melanocyte in the skin cells was collected. after the addition of estrogen and testosterone, the culture was analyzed for expression of cell activity and melanin genes for 72 hours. For the analysis of estrogen in different coat color other than the melanogenesis-related genes it is increasingly yellow showed low expression. in particular, the cells of the brindle coat color is low active and expression of genes. However, the testosterone was low, the expression of cell activity inhibiting MMP-2. the expression of melanin genes actually showed a tendency to increase gradually, which is testosterone compared with the estrogen to be considered that affect the skin cell layer brindle coat color. In this study, stimulation with estrogen triggered the inhibition of MC1R of the melanocyte in brindle coat color, but testosterone is induced MC1R in melanocyte. Therefore, considered the eumelanin or phaeomelanin activation are controlled caused by differential expression of sex hormones on melanocyte in Korean Brindle Cattle.

Keywords

References

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