대한의생명과학회지 (Biomedical Science Letters)

  • 제24권3호
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  • Pages.221-229
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  • 2018
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
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Genetic Variations of Trichophyton rubrum Clinical Isolates from Korea

  • Yoon, Nam-Sup (Department of Laboratory Medicine, Asan Medical Center) ;
  • Kim, Hyunjung (QuantaMatrix, Inc.) ;
  • Park, Sung-Bae (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Park, Min (Department of Biomedical Laboratory Science, Daekyung University) ;
  • Kim, Sunghyun (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Kim, Young-Kwon (Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University)
  • 투고 : 2018.08.17
  • 심사 : 2018.09.13
  • 발행 : 2018.09.30
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초록

Trichophyton rubrum is one of the well-known pathogenic fungi and causes dermatophytosis and cutaneous mycosis in human world widely. However, there are not an available sequence type (ST) classification methods and previous studies for T. rubrum until now. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to characterize the genetic diversity and the phylogenetic relation of T. rubrum clinical isolates, five different housekeeping genes, such as actin (ACT), calmodulin (CAL), RNA polymerase II (RPB2), superoxide dismutase 2 (SOD2), and ${\beta}$-tubulin (BT2) were analyzed using by multilocus sequence typing (MLST). Also, DNA sequence analysis was performed to examine the differences between the sequences of Trichophyton strains and the identified genetic variations sequence. As a result, most of the sequences were shown to have highly matched rates in their housekeeping genes. However, genetic variations were found on three different positions of ${\beta}$-tubulin gene and were shown to have changed from $C{\rightarrow}G$ (1766), $G{\rightarrow}T$ (1876), and $C{\rightarrow}A$ (1886). To confirm the association with T. rubrum inheritance, a phylogenetic tree analysis was performed. It was classified as four clusters, but there was little significant correlation. Even so, MLST analysis is believed to be helpful for determining the genetic variations of T. rubrum in cases where there is more large-scale data accumulation. In conclusion, the present study demonstrated the first MLST analysis of T. rubrum in Korea and explored the possibility that MLST could be a useful tool for studying the epidemiology and evolution of T. rubrum through further studies.

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참고문헌

  1. Anderson MJ, Gull K, Denning DW. Molecular typing by random amplification of polymorphic DNA and M13 Southern hybridization of related paired isolates of Aspergillus fumigatus. Journal of Clinical Microbiology. 1996. 34: 87-93.
  2. Anne D, Cecile G, Christophe H, Nelly CA, Sybren de H, Marie M. Development of a new MLST scheme for differentiation of Fusarium solani species complex (FSSC) isolates. Journal of Microbiological Methods. 2010. 82: 319-323. https://doi.org/10.1016/j.mimet.2010.07.008
  3. Atef SS, Pranab KM, Hassan NA, Atef I, El A, Said HA, Mahmoud AG. Single-Step PCR Using (GACA) 4 Primer: Utility for Rapid Identification of Dermatophyte Species and Strains. Journal of Clinical Microbiology. 2008. 46: 2641-2645. https://doi.org/10.1128/JCM.00697-08
  4. Balajee SA, Nickle D, Varga J, Marr KA. Molecular studies reveal frequent misidentification of Aspergillus fumigatus by morphotyping. Eukaryotic Cell. 2006. 5: 1705-1712. https://doi.org/10.1128/EC.00162-06
  5. Bernhardt A, Sedlacek L, Wagner S, Schwarz C, Wurstl B, Tintelnot K. Multi locus sequence typing of Scedosporium apiospermum and Pseudallescheria boydii isolates from cystic fibrosis patients. Journal of Cystic Fibrosis. 2013. 12: 592-598. https://doi.org/10.1016/j.jcf.2013.05.007
  6. Bidet P, Lalande V, Salauze B, Burghoffer B, Avesani V, Delmee M, et al. Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile. Journal of Clinical Microbiology. 2000. 38: 2484-2487.
  7. Bougnoux, ME, Tavanti A, Bouchier C, Gow NAR, Magnier A, Davidson AD, et al. Collaborative consensus for optimized multilocus sequence typing of Candida albicans. Journal of Clinical Microbiology. 2003. 41: 5265-5266. https://doi.org/10.1128/JCM.41.11.5265-5266.2003
  8. Byun JH, Yoo JH, Park CM, Lee Dg, Park SH, Choi SM. Molecular epidemiologic analysis of community-onset extended spectrum beta-lactamase (ESBL) producing Escherichia coli using infrequent-restriction site polymerase chain reaction (IRS-PCR) with comparison by pulsed-field gel electrophoresis (PFGE). Infect Chemotherapy. 2012. 44: 5-10. https://doi.org/10.3947/ic.2012.44.1.5
  9. Dodgson AR, Pujol C, Denning DW, Soll DR, Fox AJ. Multilocus sequence typing of Candida glabrata reveals geographically enriched clades. Journal of Clinical Microbiology. 2003. 41: 5709-5717. https://doi.org/10.1128/JCM.41.12.5709-5717.2003
  10. Guofang L, Chenghua HE, Haibin Z. Identification and characterization of dermatophyte species and strains with PCR amplification. Experimental and Therrapeutic Medicine. 2014. 8: 545-550. https://doi.org/10.3892/etm.2014.1785
  11. Jin H, Kim H, Kim S, Choi Y, Bang H, Park S, Wang H, Lee JH, Jang IH, Kim YK, Lee H. Evaluation of a PCR-Reverse Blot Hybridization Assay to Identify Six Dermatophytes Predominant in the Republic of Korea. Biomedical Science Letters. 2014. 20: 139-146. https://doi.org/10.15616/BSL.2014.20.3.139
  12. John WT, Matthew CF. Fungal multilocus sequence typing-it's not just for bacteria. Current Opinion Microbiology. 2003. 6: 351-356. https://doi.org/10.1016/S1369-5274(03)00088-2
  13. Judy RR, Robert WP, Rana AH, Mary EB, Arthur LR. The epidemiological features of invasive mycotic infections in the San Francisco bay area, 1992-1993: Results of population-based laboratory activity surveillance. Clinical Infectious Diseases. 1998. 27: 1138-1147. https://doi.org/10.1093/clinids/27.5.1138
  14. Kim KH, Jun JB, Yoo HJ. Superficial and cutaneous mycoses. Dermatology revised vol. 4. Seoul: Ryo Moon Gak. 2001. 310-340.
  15. Kim H, Jin H, Kim S, Wang H, Choi Y, Bang H, Park JS, Lee JH, Won YH, Ahn KJ, Kim YK, Lee H. PCR-reverse Blot Hybridization Assay for Species Identification of Dermatophytes. Korean Journal of Medical Mycology. 2011. 16: 86-98.
  16. Kim SJ, Kim SJ. A Distribution of Keratinophilic Fungi Isolated from the Soil of Haeundae Beach in Korea, Korean Journal of Clinical Laboratory Science. 2016. 48: 343-347. https://doi.org/10.15324/kjcls.2016.48.4.343
  17. Kwon-Chung KJ, Bennett JE. Philadelphia: Lea & Febigger. Medical mycology. 1992. 105-161.
  18. Lee SK, Choi JS, Kim KH. A clinical and mycological Study of Tinea Pedis. Annals of Dermatology. 1995. 33: 1029-1037.
  19. Lee WJ, Kim SL, Jang YH, Lee SJ, Kim DW, Bang YJ, et al. Increasing Prevalence of Trichophyton rubrum Identified through an Analysis of 115,846 Cases over the Last 37 Years. Journal of Korean Medical Science. 2015. 30: 639-643. https://doi.org/10.3346/jkms.2015.30.5.639
  20. Litvintseva AP, Thakur R, Vilgalys R, Mitchell TG. Multilocus sequence typing reveals three genetic subpopulations of Cryptococcus neoformans var. grubii (serotype A), including a unique population in Botswana. Genetics. 2006. 172: 2223-2238.
  21. Oh BH, Ahn KJ. Drug Therapy of Dermatophytosis. Journal of the Korean Medical Association. 2009. 52: 1109-1114. https://doi.org/10.5124/jkma.2009.52.11.1109
  22. Semighini CP, Delmas G, Park S, Amstrong D, Perlin D, Goldman GH. New restriction fragment length polymorphism (RFLP) markers for Aspergillus fumigatus. FEMS Immunology and Medical Microbiology. 2001. 31: 15-19. https://doi.org/10.1111/j.1574-695X.2001.tb01580.x
  23. Vikesh KB, Prakash CS. Epidemiological studies on Dermatophytosis in human patients in Himachal Pradesh. India: Springer-Plus. 2014. 134.
  24. Weitzman I, Summerbell RC. The dermatophytes. Clinical Microbiology Reviews. 1995. 8: 240-259. https://doi.org/10.1128/CMR.8.2.240
  25. Yang JH. Statistical observation of skin fungal disease for last 10 years. Korean Dermatology & Urology Society Newsletter. 1949. 1: 10-78.
  26. Yoon HJ, Choi HY, Kim YK, Song YJ, Ki Moran. Prevalence of fungal infections using National Health Insurance data from 2009-2013. Epidemiology and Health. 2014. 36.