Protective effect of ethanolic extract of antler-shaped Ganoderma lingzhi against oxidative stress in PC12 neuronal cell line

PC12 신경세포주에서 녹각영지버섯 주정 추출물의 산화 스트레스 개선 효과

This study was carried out to identify medicinal mushrooms with protective effects against oxidative stress in PC12 neuronal cell line, followed by evaluation of their antioxidant property. Extracts of medicinal mushrooms, including Ganoderma lucidum extract (GLE), antler-shaped Ganoderma lingzhi extract (AGLE), Hericium erinaceus extract (HEE), and Sanghuangporus baumii extract (SBE), were screened for cytotoxicity using MTT assay. None of the extracts up to $10{\mu}g/ml$ concentration affected cell viability. These extracts were further checked for their protective effect against oxidative stress-induced reactive oxygen species (ROS) production. Exposure to $50{\mu}M$ $H_2O_2$ induced ROS generation in PC12 cells, which was inhibited only by treatment with AGLE. In addition, inhibition of $H_2O_2-induced$ ROS generation by AGLE was found to be in a dose-dependent manner (2.5, 5, and $10{\mu}g/ml$). Microscopic examination of DCF fluorescence for detection of ROS showed a similar pattern. Further, antioxidant activity of AGLE was determined by ABTS radical cation assay, and its $IC_{50}$ was found to be $46.90{\pm}0.31{\mu}g/ml$. Taken together, these results suggest that AGLE may help to alleviate oxidative stress in PC12 neuronal cells.

알츠하이머병, 파킨슨병 등 신경퇴행성질환은 ROS 축적에 의한 산화 스트레스가 주된 원인 중 하나인 것으로 알려져 있다. 따라서 본 연구에서는 신경세포 내 ROS 생성을 억제하는 약용 버섯 추출물을 탐색하여, 녹각영지버섯 추출물을 선발하였다. 실험 결과, PC12 신경세포에서 $H_2O_2$ $50{\mu}M$ 처리에 의해 증가한 ROS 생성이 녹각영지버섯 추출물에 의해 억제되었다. 녹각영지버섯 추출물은 우수한 ABTS 라디칼 소거능($IC_{50}:46.90{\mu}g/ml$)을 가지고 있었으며, 신경세포에서 산화자극에 의한 ROS 생성을 농도의존적으로 억제하였다. 이러한 녹각영지버섯 추출물의 ROS 생성 억제능은 형광현미경 관찰을 통해서도 확인하였다. 본 연구의 실험 결과들은 녹각영지버섯의 항치매 기능성 소재로서의 가치를 보여주는 결과라고 할 수 있다.