Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Comparison of histopathological and molecular diagnostic methods in Helicobacter suis infection in Korea

국내 도축돈의 위병변과 Helicobacter suis의 검출과의 상관관계

  • Lee, Jae Ha (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Lee, Sun Gue (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Han, Jeong Hee (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 이재하 (강원대학교 수의과대학 및 동물의학연구소) ;
  • 이선규 (강원대학교 수의과대학 및 동물의학연구소) ;
  • 한정희 (강원대학교 수의과대학 및 동물의학연구소)
  • Received : 2018.09.10
  • Accepted : 2018.09.16
  • Published : 2018.09.30
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Abstract

Helicobacter suis is a gram negative bacterium and colonizes in porcine stomach. It causes gastric diseases in the stomach and plays a significant role in daily weight gains in pigs. Recent studies about one of potential sources of human gastric diseases. Therefore, this study was conducted to compare histopathological lesions and molecular detection of Helicobacter suis in the pyloric mucosa of porcine stomachs transferred from slaughterhouses, based on gross and histological examinations and a PCR assay. A total 90 stomach samples were investigated to record gastric lesion scores by characteristic gastric lesions, followed by routine H & E and Warthin-Starry silver staining to detect Helicobacter-like organisms. For PCR assay, H. suis specific primers and conditions are used. Sixty-one samples (67.8%) showed gross gastric lesions, of which 38 samples (40.2%) presented grade 1, 12 samples (13.3%) presented grade 2, and 11 samples (12.2%) presented grade 3, respectively. In Warthin-Starry silver stain, Helicobacter-like organisms were detected from 11 samples (12.2%) with 4 samples (4.4%) for grade 0, 5 samples (5.6%) for grade 1, 1 sample (1.1%) for grade 2 and 1 sample (1.1%) for grade 3, respectively. The PCR resulted positive in 37 samples (41.1%) with 14 samples (15.6%) for grade 0, 14 samples (15.6%) for grade 1, 3 samples (3.3%) for grade 2 and 6 samples (6.7%) for grade 3, respectively. Positive samples for both examinations were 5 samples (5.6%). The result suggested that it should be considered as one of factors causing a gastric disease in pigs. Also, it could be acknowledged to research fundamental aspects of Helicobacter-induced gastritis in human as an animal model.

Keywords

References

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