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Platycodi radix beverage ameliorates postprandial lipemia response through lipid clearance of triglyceride-rich lipoprotein: A randomized controlled study in healthy subjects with a high-fat load

  • Lee, Hansol (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Lim, Yeni (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Park, Soo-yeon (Department of Nutritional Science and Food Management, Ewha Womans University) ;
  • Cho, Soo-Muk (Department of Agrofood Resources, Rural Development Administration National Institute of Agricultural Sciences) ;
  • Choe, Jeong-Sook (Department of Agrofood Resources, Rural Development Administration National Institute of Agricultural Sciences) ;
  • Jeong, Sewon (BiofoodCRO. Ltd.) ;
  • Kwak, Jin Sook (BiofoodCRO. Ltd.) ;
  • Kwon, Oran (Department of Nutritional Science and Food Management, Ewha Womans University)
  • Received : 2018.05.18
  • Accepted : 2018.07.25
  • Published : 2018.10.01

Abstract

BACKGROUND/OBJECTIVES: Elevation of postprandial lipemia characterized by a rise in triglyceride (TG)-rich lipoproteins can increase the risk of atherogenesis. The objective of this study was to investigate postprandial lipemia response to a single dietary fat/sugar load test and monitor beneficial changes induced by the consumption of Platycodi radix (AP) beverage in healthy subjects. SUBJECTS/METHODS: A total of 52 subjects were randomly assigned to either placebo or AP beverage group with a high-fat shake in a randomized controlled crossover trial. Postprandial blood was collected at 0, 1, 2, 4, and 6 h and analyzed for TG and lipoprotein lipase mass. Inhibition of pancreatic lipase was determined in vitro. RESULTS: AP inhibited pancreatic lipase activity in vitro ($IC_{50}=5mg/mL$). Compared to placebo beverage, AP beverage consumption with a high-fat shake induced significant increase of plasma lipoprotein lipase mass (P = 0.0111, ${\beta}$ estimate = 4.2948) with significant reduction in very low-density lipoprotein (VLDL) TG concentration (P = 0.038, ${\beta}$ estimate = -52.69) at 6 h. Based on significant correlation between high-fat dietary scores MEDFICTS and postprandial TG responses in VLDL (P = 0.0395, r = 0.2127), subgroup analysis revealed that 6 h-postprandial VLDL TG response was significantly decreased by AP consumption in subjects with MEDFICTS ${\geq}40$ (P = 0.0291, ${\beta}$ estimate = -7214). CONCLUSIONS: AP beverage might have potential to alleviate postprandial lipemia through inhibiting pancreatic lipase activity and elevating lipoprotein lipase mass. Subgroup analysis revealed that subjects with high-fat dietary pattern could be classified as responders to AP beverage among all subjects.

Keywords

References

  1. Hyson D, Rutledge JC, Berglund L. Postprandial lipemia and cardiovascular disease. Curr Atheroscler Rep 2003;5:437-44. https://doi.org/10.1007/s11883-003-0033-y
  2. Bansal S, Buring JE, Rifai N, Mora S, Sacks FM, Ridker PM. Fasting compared with nonfasting triglycerides and risk of cardiovascular events in women. JAMA 2007;298:309-16. https://doi.org/10.1001/jama.298.3.309
  3. Dias CB, Moughan PJ, Wood LG, Singh H, Garg ML. Postprandial lipemia: factoring in lipemic response for ranking foods for their healthiness. Lipids Health Dis 2017;16:178. https://doi.org/10.1186/s12944-017-0568-5
  4. Lopez-Miranda J, Williams C, Lairon D. Dietary, physiological, genetic and pathological influences on postprandial lipid metabolism. Br J Nutr 2007;98:458-73. https://doi.org/10.1017/S000711450774268X
  5. Lee EB. Pharmacological studies on Platycodon grandiflorum A. DC. IV. A comparison of experimental pharmacological effects of crude platycodin with clinical indications of platycodi radix. Yakugaku Zasshi 1973;93:1188-94. https://doi.org/10.1248/yakushi1947.93.9_1188
  6. Song JY, Lee GA, Yoon MS, Ma KH, Choi YM, Lee JR, Park HJ, Lee MC. Development and characterization of 22 polymorphic micro- satellite markers for the balloon flower Platycodon grandiflorum (Campanulaceae). Genet Mol Res 2012;11:3263-6. https://doi.org/10.4238/2012.September.12.9
  7. Han LK, Xu BJ, Kimura Y, Zheng Y, Okuda H. Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. J Nutr 2000;130:2760-4. https://doi.org/10.1093/jn/130.11.2760
  8. Han LK, Zheng YN, Xu BJ, Okuda H, Kimura Y. Saponins from platycodi radix ameliorate high fat diet-induced obesity in mice. J Nutr 2002;132:2241-5. https://doi.org/10.1093/jn/132.8.2241
  9. Zhao HL, Cho KH, Ha YW, Jeong TS, Lee WS, Kim YS. Cholesterol- lowering effect of platycodin D in hypercholesterolemic ICR mice. Eur J Pharmacol 2006;537:166-73. https://doi.org/10.1016/j.ejphar.2006.03.032
  10. Kim JY, Yang YJ, Yang YK, Oh SY, Hong YC, Lee EK, Kwon O. Diet quality scores and oxidative stress in Korean adults. Eur J Clin Nutr 2011;65:1271-8. https://doi.org/10.1038/ejcn.2011.120
  11. Redgrave TG, Carlson LA. Changes in plasma very low density and low density lipoprotein content, composition, and size after a fatty meal in normo- and hypertriglyceridemic man. J Lipid Res 1979;20: 217-29.
  12. Xu BJ, Han LK, Zheng YN, Lee JH, Sung CK. In vitro inhibitory effect of triterpenoidal saponins from Platycodi Radix on pancreatic lipase. Arch Pharm Res 2005;28:180-5. https://doi.org/10.1007/BF02977712
  13. Purcell R, Latham SH, Botham KM, Hall WL, Wheeler-Jones CP. High-fat meals rich in EPA plus DHA compared with DHA only have differential effects on postprandial lipemia and plasma 8-isoprostane $F2{\alpha}$ concentrations relative to a control high-oleic acid meal: a randomized controlled trial. Am J Clin Nutr 2014;100:1019-28. https://doi.org/10.3945/ajcn.114.091223
  14. McCrea CE, West SG, Kris-Etherton PM, Lambert JD, Gaugler TL, Teeter DL, Sauder KA, Gu Y, Glisan SL, Skulas-Ray AC. Effects of culinary spices and psychological stress on postprandial lipemia and lipase activity: results of a randomized crossover study and in vitro experiments. J Transl Med 2015;13:7. https://doi.org/10.1186/s12967-014-0360-5
  15. Thomsen C, Rasmussen O, Lousen T, Holst JJ, Fenselau S, Schrezenmeir J, Hermansen K. Differential effects of saturated and monounsaturated fatty acids on postprandial lipemia and incretin responses in healthy subjects. Am J Clin Nutr 1999;69:1135-43. https://doi.org/10.1093/ajcn/69.6.1135
  16. Westphal S, Orth M, Ambrosch A, Osmundsen K, Luley C. Postprandial chylomicrons and VLDLs in severe hypertriacylglycerolemia are lowered more effectively than are chylomicron remnants after treatment with n-3 fatty acids. Am J Clin Nutr 2000;71:914-20. https://doi.org/10.1093/ajcn/71.4.914
  17. Wong H, Schotz MC. The lipase gene family. J Lipid Res 2002;43: 993-9. https://doi.org/10.1194/jlr.R200007-JLR200
  18. Birari RB, Bhutani KK. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today 2007;12:879-89. https://doi.org/10.1016/j.drudis.2007.07.024
  19. Loli H, Narwal SK, Saun NK, Gupta R. Lipases in medicine: an overview. Mini Rev Med Chem 2015;15:1209-16. https://doi.org/10.2174/1389557515666150709122722
  20. Zhao HL, Kim YS. Determination of the kinetic properties of platycodin D for the inhibition of pancreatic lipase using a 1,2-diglyceride-based colorimetric assay. Arch Pharm Res 2004;27: 968-72. https://doi.org/10.1007/BF02975852
  21. Otarod JK, Goldberg IJ. Lipoprotein lipase and its role in regulation of plasma lipoproteins and cardiac risk. Curr Atheroscler Rep 2004;6:335-42. https://doi.org/10.1007/s11883-004-0043-4
  22. Kris-Etherton P, Eissenstat B, Jaax S, Srinath U, Scott L, Rader J, Pearson T. Validation for MEDFICTS, a dietary assessment instrument for evaluating adherence to total and saturated fat recommen- dations of the National Cholesterol Education Program Step 1 and Step 2 diets. J Am Diet Assoc 2001;101:81-6. https://doi.org/10.1016/S0002-8223(01)00020-7

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