초록
국내에서 재배되는 옥수수의 수확 후 버려지는 잎을 수거하여 음건하고 분쇄 한 후 실험에 사용하였다. 두 가지 용매로 추출 후 DPPH, ABTS, 총 플라보노이드 함량, 총 폴리페놀 함량을 측정하여 항산화 활성을 분석하고, Paper disc diffusion method 로 clear zone의 직경을 측정 하였다. 그 결과 에탄올 추출물이 모든 항산화활성과 항균활성에서 다소 높은 결과를 나타내었다. Gallic acid를 표준물질로 하여 총 polyphenol 함량을 측정한 결과 CE는 $31.2{\pm}5.2mg\;GAE/g$로 높은 활성을 보였고, DPPH radical 소거능 측정 결과 CE는 1, 10, 100, 1,000 (${\mu}g/mL$) 농도에서 각각 $4.6{\pm}0.9%$, $6.4{\pm}0.5%$, $22.9{\pm}0.6%$, $83.2{\pm}0.2%$로 나타나 농도 의존적 경향을 보였으며 1,000 (${\mu}g/mL$)에서는 매우 높은 활성을 보였다. 지금까지 옥수수와 옥수수 수염에 대한 연구는 많지만 페기 되는 잎에 대한 연구는 없어 본 연구가 새로운 천연 항산화, 항균 효능을 가진 원료개발의 기초 자료가 될 것으로 판단된다.
In this study, Zea mays L., which were discarded after harvesting in Korea, were collected and used for experiment. The Zea mays L. were dried in the shade and ground before being extracted using two solvents. Subsequently, DPPH, ABTS, the total flavonoid content and the total polyphenol content were measured to analyze antioxidant activity. The diameter of clear zone was measured by using the paper disc diffusion method. The results showed that the ethanol extract had a slightly higher antioxidant and antibacterial activity. The total polyphenol content was measured using gallic acid as standard, and CE(ethanol extract) was found to contain $31.2{\pm}5.2mg\;GAE/g$ of polyphenols, indicating a considerably high activity. When DPPH radical scavenging ability was measured, CE was found to have $4.6{\pm}0.9%$, $6.4{\pm}0.5%$, $22.9{\pm}0.6%$ and $83.2{\pm}0.2%$ at different concentrations of $1{\mu}g/mL$, $10{\mu}g/mL$, $100{\mu}g/mL$, and $1,000{\mu}g/mL$, respectively, with a dependent tendency at the concentration of $1,000{\mu}g/mL$ and a considerably high activity at the concentration of $1,000{\mu}g/mL$. This study is expected to be used as preliminary data to develop new natural antioxidant, antibacteria raw materials, as only little research has been undertaken on Zea mays L., which are mostly discarded after harvesting, while many studies have been so far conducted on corn and corn silks.