Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Complete genome sequence of the polycyclic aromatic hydrocarbons biodegrading bacterium Idiomarina piscisalsi strain 10PY1A isolated from oil-contaminated soil

기름으로 오염된 토양에서 분리된 다환방향족탄화수소 분해 세균 Idiomarina piscisalsi 10PY1A의 유전체 염기서열 해독

  • Nzila, Alexis (Department of Life Sciences, King Fahd University of Petroleum and Minerals) ;
  • Jung, Byung Kwon (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Min-Chul (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Ibal, Jerald Conrad (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Budiyanto, Fitri (Department of Life Sciences, King Fahd University of Petroleum and Minerals) ;
  • Musa, Musa M. (Department of Chemistry, King Fahd University of Petroleum and Minerals) ;
  • Thukair, Assad (Department of Life Sciences, King Fahd University of Petroleum and Minerals) ;
  • Kim, Sang-Jun (Department of Natural Sciences, Republic of Korea Naval Academy) ;
  • Shin, Jae-Ho (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • ;
  • 정병권 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김민철 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • ;
  • ;
  • ;
  • ;
  • 김상준 (해군사관학교 자연과학부) ;
  • 신재호 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2018.06.01
  • Accepted : 2018.07.03
  • Published : 2018.09.30
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Abstract

Using pyrene as the enrichment nutrient, a bacterial strain 10PY1A, was isolated by enrichment culture from oil-contaminated sea sand of Arabian Gulf in Saudi Arabia, and this strain belongs to the species Idiomarina piscisalsi, based on 16S RNA gene sequence analysis. The genome of I. piscisalsi strain 10PY1A contains 2,346 protein-coding sequences and an average GC content of 47.4% in its chromosome (2.59 Mbp). Genes encoding proteins related to the degradation of pyrene were existed in the strain 10PY1A genome, indicating that this strain can be used to degrade polycyclic aromatic hydrocarbons in oil-contaminated marine flora and soil.

석유로 오염된 사우디아라비아의 바다 모래에 pyrene을 영양물로 첨가하는 집식배양을 통해 우점하는 박테리아 균주 10PY1A가 분리되었다. 이 균주는 16S rRNA 유전자 분석을 통해 Idiomarina piscisalsi로 동정되었다. 이 균주는 G + C 비율이 47.4%이며 2.69 Mbp 크기의 원형 염색체를 보유하고 있으며, 해당 염색체는 다환방향족탄화수소 분해와 관련 있는 유전자를 포함한 2,346개의 단백질 코딩 유전자로 구성되어 있다. 이는 이 균주가 석유로 오염된 해양과 토양에서 방향족탄화수소를 분해하는 데 사용될 수 있음을 보여준다.

Keywords

References

  1. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, et al. 2008. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9, 75. https://doi.org/10.1186/1471-2164-9-75
  2. Bostrom CE, Gerde P, Hanberg A, Jernstrom B, Johansson C, Kyrklund T, Rannug A, Tornqvist M, Victorin K, and Westerholm R. 2002. Cancer risk assessment, indicators, and guidelines for polycyclic aromatic hydrocarbons in the ambient air. Environ. Health Perspect. 110, 451-488.
  3. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, Clum A, Copeland A, Huddleston J, Eichler EE, et al. 2013. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat. Methods 10, 563-569. https://doi.org/10.1038/nmeth.2474
  4. Eid J, Fehr A, Gray J, Luong K, Lyle J, Otto G, Peluso P, Rank D, Baybayan P, Bettman B, et al. 2009. Real-time DNA sequencing from single polymerase molecules. Science 323, 133-138. https://doi.org/10.1126/science.1162986
  5. Fakhru'l-Razi A, Pendashteh A, Abdullah LC, Biak DRA, Madaeni SS, and Abidin ZZ. 2009. Review of technologies for oil and gas produced water treatment. J. Hazard Mater. 170, 530-551. https://doi.org/10.1016/j.jhazmat.2009.05.044
  6. Oppermann UCT and Maser E. 2000. Molecular and structural aspects of xenobiotic carbonyl metabolizing enzymes. Role of reductases and dehydrogenases in xenobiotic phase I reaction. Toxicology 144, 71-81. https://doi.org/10.1016/S0300-483X(99)00192-4
  7. Sierra-Garcia IN, Alvarez JC, de Vasconcellos SP, de Souza AP, dos Santos Neto EV, and de Oliveira VM. 2014. New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs. PLoS One 9, e90087. https://doi.org/10.1371/journal.pone.0090087

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