미생물학회지 (Korean Journal of Microbiology)

  • 제54권3호
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  • Pages.222-227
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  • 2018
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
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수지상세포의 CD1b 분자와 포식작용의 증가

CD1b in immature dendritic cells acquires increased phagocytotic function

  • 류현정 (국민대학교 과학기술대학 바이오발효합학과)
  • Liew, Hyunjeong (Department of Bio & Fermentation Convergence, Kookmin University)
  • 투고 : 2018.08.28
  • 심사 : 2018.08.30
  • 발행 : 2018.09.30
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초록

CD1 분자는 결핵균 유래 지질항원 발현하는 단백질이며, 특히 수지상세포(dendritic cells)가 결핵균 감염시에 발현이 점차 감소함을 관찰하였다. 이는 결핵균의 사균이나 항원만으로는 관찰되지 않는 결과였다. 2차원 전기영동(2D electrophoresis)을 통하여 CD1b 의 인산화를 관찰하였고 이러한 현상이 식세포작용과 연관됨을 확인하였다.

Mycobacterium tuberculosis (MTB)-originated lipid antigen is presented on the antigen-presenting cell surface with CD1b. When monocyte-derived dendritic cells phagocytosed MTB H37Rv (Multiplicity of infection 10, infectivity: 46.89%), the CD1b expression level decreased slowly. Since this was just a live MTB-mediated phenomenon, it was not detected from heat-killed MTB or mycolic acid, which is a unique antigen of MTB. We confirmed that the phosphorylation of CD1b molecules using 2D electrophoresis with staining could phosphorylate and induce the presentation of the lipid antigen using the phagocytosis assay.

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