Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Diagnosis of Clostridium difficile infection in patients with hospital-acquired diarrhea

  • Ibrahim Afifi, Salwa Selim (Faculty of Pharmacy-Girls, Al-Azhar University, Microbiology and Immunology) ;
  • Gomaa, Fatma Alzahraa M. (Faculty of Pharmacy-Girls, Al-Azhar University, Microbiology and Immunology) ;
  • Fathi, Lamia Fouad (Ain Shams Faculty of Medicine, Medical Microbiology and Immunology) ;
  • Rasslan, Fatma Salah (Faculty of Pharmacy-Girls, Al-Azhar University, Microbiology and Immunology) ;
  • Hamdy, Ahmed Mohamed (Ain Shams Faculty of Medicine, Pediatrics)
  • Received : 2018.07.30
  • Accepted : 2018.08.27
  • Published : 2018.09.30
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Abstract

Clostridium difficile infection (CDI) is a rapidly emerging infection that may have devastating consequences. Prompt and accurate diagnosis is crucial for management and control. The aim of this study was to determine the incidence of C. difficile associated diarrhea among hospitalized patients, and to compare different diagnostic laboratory methods for detection of toxin producing strains in clinical specimens. The study was conducted at a university hospital in Cairo during the period from May 2013 till June 2015. Subjects were under antibiotic therapy and presented with hospital-acquired diarrhea. Four hundred and sixty-five stool specimens were processed by different microbiological methods. C. difficile was recovered in culture in 51 of stool specimens. Of these, 86.3% to 98% were positive for toxin production by 2 different methods. This study showed that antibiotic intake is the major risk factor for development of hospital-acquired diarrhea. We evaluated different microbiological methods for diagnosis of C. difficile. We recommend the use of toxigenic culture as a gold standard for microbiological diagnosis of C. difficile.

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References

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