Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Analysis of Essential oil, Quantification of Six Glycosides, and Nitric Oxide Synthase Inhibition Activity in Caryopteris incana

  • Nugroho, Agung (Department of Agro-industrial Technology, Lambung Mangkurat University) ;
  • Lee, Sang Kook (College of Pharmacy, Seoul National University) ;
  • Kim, Donghwa (College of Pharmacy, Seoul National University) ;
  • Choi, Jae Sue (Department of Food and Nutrition, Pukyung National University) ;
  • Park, Kyoung-Sik (Department of Oriental Medicine, Sangji University) ;
  • Song, Byong-Min (Department of Forest Science, Sangji University) ;
  • Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University)
  • Received : 2018.04.04
  • Accepted : 2018.04.27
  • Published : 2018.09.30
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Abstract

Caryopteris incana (Verbenaceae) has been used to treat cough, arthritis, and eczema in Oriental medicine. The two fractions ($CHCl_3-$ and BuOH fractions) and the essential oil of the plant material were subjected to the inducible nitric oxide synthase (iNOS) assay. The $IC_{50}$ of the $CHCl_3$ fraction and the essential oil on LPS-induced macrophage RAW 264.7 cells were $16.4{\mu}g/mL$ and $23.08{\mu}g/mL$, respectively. On gas chromatography (GC)-mass spectroscopy (MS) analysis, twenty-five components representing 85.5% amount of total essential oil were identified. On the chromatogram, three main substances, trans-pinocarveol, cis-citral, and pinocarvone, occupied 18.8%, 13.5% and 18.37% of total peak area. Furthermore, by HPLC-UV analysis, six compounds including one iridoid (8-O-acetylharpagide)- and five phenylethanoid glycosides (caryopteroside, acteoside, phlinoside A, 6-O-caffeoylphlinoside, and leucosceptoside A) isolated from the BuOH fraction were quantified. The content of six compounds were shown as the following order: caryopteroside (162.35 mg/g) > 8-O-acetylharpagide (93.28 mg/g) > 6-O-caffeoylphlinoside (28.15 mg/g) > phlinoside (22.60 mg/g) > leucosceptoside A (16.87 mg) > acteoside (7.05 mg/g).

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