한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제22권4호
  • /
  • Pages.393-402
  • /
  • 2018
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Genetic Distances in Two Gracilaria Species (Gracilariaceae, Rhodophyta) Identified by PCR Technique

  • Kim, Young Sik (Dept. of Marine Life Technology, College of Ocean Science and Technology, Kunsan National University) ;
  • Yoon, Jong-Man (Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 투고 : 2018.10.17
  • 심사 : 2018.11.13
  • 발행 : 2018.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Genomic DNA was isolated from the Gracilaria vermiculophylla (GRV) and G. chorda (GRC) from Jangheung located in the southern sea of the Korean Peninsula, respectively and we performed clustering analyses, DNA polymorphisms and the genetic differences. The seven selected primers OPC-01, OPA-04, OPA-05, OPD-07, OPD-08, OPB-10, and OPD-16 generated average bandsharing (BS) value, the genetic distance and dendrogram. The size of DNA bands varies from 90 bp to 2,400 bp. The average BS value was $0.859{\pm}0.004$ within GRV and $0.916{\pm}0.006$ within GRC. The average BS value between two Gracilaria species was $0.340{\pm}0.003$, ranged from 0.250 to 0.415. The dendrogram obtained by the seven primers, indicates two genetic clusters. The genetic distance between two Gracilaria species ranged from 0.059 to 0.513. The individual VERMICULOPHYLLA no. 07 of GRV was genetically closely related to VERMICULOPHYLLA no. 06 of GRV (genetic distance=0.059). Especially, two entities between the individual VERMICULOPHYLLA no. 10 of GRV and CHORDA no. 22 of GRC showed the longest genetic distance (0.513) in comparison with other individuals used. Accordingly, as mentioned above, PCR analysis showed that the GRV was a little more genetically diverse than the GRC species. We convinced that this DNA analysis revealed a significant genetic distance between two Gracilaria species pairs (p<0.01).

키워드

참고문헌

  1. Adams RP (2000) Systematics of the one seeded Juniperus of the eastern hemisphere based on leaf essential oils and random amplified polymorphic DNAs (RAPDs). Biochem Syst Ecol 28:529-543. https://doi.org/10.1016/S0305-1978(99)00096-4
  2. Bartish IV, Garkava LP, Rumpunen K, Nybom H (2000) Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes. Theor Appl Genet 101:554-563. https://doi.org/10.1007/s001220051515
  3. Bommineni VR, Jauhar PP, Peterson TS, Chibbar RN, Almouslem AB (1997) Analysis of hybrids of durum wheat with Thinopyrum juncelforme using RAPD markers. Theor Appl Genet 95:757-763. https://doi.org/10.1007/s001220050622
  4. Callejas C, Ochando MD (1998) Identification of Spanish barbel species using the RAPD technique. J Fish Biol 53:208-215. https://doi.org/10.1111/j.1095-8649.1998.tb00121.x
  5. Esselman EJ, Crawford DJ, Brauner S, Stuessy TF, Anderson GJ, Silva OM (2000) RAPD marker diversity within and divergence among species of Dendroseris (Asteraceae: Lactuceae). Am J Bot 87:591-596. https://doi.org/10.2307/2656603
  6. Eujayl I, Baum M, Powell W, Erskine W, Pehu E (1998) A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines. Theor Appl Genet 97:83-89. https://doi.org/10.1007/s001220050869
  7. Hong YK, Sohn CH, Lee KW, Kim HG (1997) Nucleic acid extraction from seaweed tissues for polymerase chain reaction. J Mar Biotechnol 5:95-99.
  8. Iyengar A, Piyapattanakorn S, Stone DM, Heipel DA, Howell BR, Baynes SM, Maclean N (2000) Identification of microsatellite repeats in turbot (Scophthalmus maximus) and dover sole (Solea solea) using a RAPDbased technique: Characterization of microsatellite markers in dover sole. Mar Biotechnol 2:49-56.
  9. Jaiswal P, Sane AP, Ranade SA, Nath P, Sane PV (1998) Mitochondrial and total DNA RAPD patterns can distinguish restorers of CMS lines in sorghum. Theor Appl Genet 96:791-796. https://doi.org/10.1007/s001220050804
  10. Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1-15.
  11. Kane M, Maeda H, Fukunaga T, Nishi K (1997) Molecular phytogenetic relationship between strains of cyanobacterial picoplankton in Lake Biwa, Japan. J Mar Biotechnol 5:41-45.
  12. Kim BK, Chung GH, Fujita Y (1997a) Random amplified polymorphic DNA (RAPD) variation in Porphytra yezoensis and P. tenera. J Aquac 10:321-326.
  13. Kim LG, Jin HJ, Kim YS, Park JY, Nam KW, Hong YK (1997b) Discrimination of two red algae Acrosorium polyneurum and A. yendoi using polymerase chain reaction technique. Korean J Fish Aquat Sci 30:585-588.
  14. Kim CK, Choi HK (2003) Genetic diversity and relationship in Korean ginseng (Panax schinseng) based on RAPD analysis. Korean J Genet 25:181-188.
  15. Kim JY, Park CY, Yoon JM (2004) Genetic differences and DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean J Genet 26:123-134.
  16. Klinbunga S, Ampayup P, Tassanakajon A, Jarayabhand P, Yoosukh W (2000a) Development of species-specific markers of the tropical oyster (Crassostrea belcheri) in Thailand. Mar Biotechnol 2:476-484.
  17. Klinbunga S, Boonyapakdee A, Pratoomchat B (2000b) Genetic diversity and species-diagnostic markers of mud crabs (Genus Scylla) in Eastern Thailand determined by RAPD analysis. Mar Biotechnol 2:180-187.
  18. Kwon YS, Ryu TH, Kim CH, Song IH, Kim KM (2004) A comparative study of the RAPD and SSR markers in establishing a genetic relationship of the various types of Cucurbita. Korean J Genet 26:115-122.
  19. Moeller DA, Schaal BA (1999) Genetic relationships among Native American maize accessions of the Great Plains assessed by RAPDs. Theor Appl Genet 99:1061-1067. https://doi.org/10.1007/s001220051415
  20. Nebauer SG, del Castillo-Agudo L, Segura J (2000) An assessment of genetic relationships within the genus Digitalis based on PCR-generated RAPD markers. Theor Appl Genet 100:1209-1216. https://doi.org/10.1007/s001220051426
  21. Nicolosi E, Deng ZN, Gentile A, Malfa SL, Continella G, Tribulato E (2000) Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet 100:1155-1166. https://doi.org/10.1007/s001220051419
  22. Nozaki T, Mishiba K, Mii M, Koba T (2000) Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B. campestris. Theor Appl Genet 101:538-546. https://doi.org/10.1007/s001220051513
  23. Oh H, Yoon JM (2014) Genetic distances of three mollusk species investigated by PCR analysis. Dev Reprod 18:43-49. https://doi.org/10.12717/DR.2014.18.1.043
  24. Orozco-Castillo C, Chalmers KJ, Waugh R, Powell W (1994) Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor Appl Genet 87:934-940. https://doi.org/10.1007/BF00225787
  25. Park JW, Cho YC, Nam BH, Jin HJ, Sohn CH, Hong YK (1998) RAPD identification of genetic variation in seaweed Hizikia fusiformis (Fucales, Phaeophyta). J Mar Biotechnol 6:62-64.
  26. Song YJ, Yoon JM (2013) Genetic differences of three Pollicipes mitella populations identified by PCR analysis. Dev Reprod 17:199-205. https://doi.org/10.12717/DR.2013.17.3.199
  27. Tassanakajon A, Pongsomboon S, Jarayabhand P, Klinbunga S, Boonsaeng V (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. J Mar Biotechnol 6:249-254.
  28. Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213-7218. https://doi.org/10.1093/nar/18.24.7213
  29. Yoke-Kqueen C, Radu S (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. J Biotechnol 127:161-166. https://doi.org/10.1016/j.jbiotec.2006.06.001
  30. Yoon JM, Park HY (2002) Genetic similarity and variation in the cultured and wild crucian carp (Carassius carassius) estimated with random amplified polymorphic DNA. Asian-Aust J Anim Sci 15:470-476. https://doi.org/10.5713/ajas.2002.470
  31. Yoon JM, Kim YH (2003a) Wide marsh clam (Corbicula spp.) populations from three sites analysed by RAPDPCR-AGE. Bull Electrochem 19:337-348.
  32. Yoon JM, Kim GW (2003b) Genetic differences between cultured and wild penaeid shrimp (Penaeus chinensis) populations analysed by RAPD-PCR. Korean J Genet 25:21-32.
  33. Yoon JM, Kim JY (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian-Aust J Anim Sci 17:1053-1061. https://doi.org/10.5713/ajas.2004.1053
  34. Yoon JM (2015) Geographical variations and genetic distances of three Saxidomus purpuratus populations ascertained by PCR analysis. Dev Reprod 19:259-264. https://doi.org/10.12717/DR.2015.19.4.259