Horticulture, Environment, and Biotechnology : HEB

  • 제59권6호
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  • Pages.805-813
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  • 2018
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  • 2211-3452(pISSN)
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  • 2211-3460(eISSN)
한국원예학회 (Korean Society of Horticultural Science)
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Identifying N sources that affect N uptake and assimilation in Vanda hybrid using 15N tracers

  • Panjama, Kanokwan (Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University) ;
  • Ohyama, Takuji (Graduate School of Natural Science and Technology, Niigata University) ;
  • Ohtake, Norikuni (Graduate School of Natural Science and Technology, Niigata University) ;
  • Sato, Takashi (Faculty of Bioresource Sciences, Akita Prefectural University) ;
  • Potapohn, Nuttha (Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University) ;
  • Sueyoshi, Kuni (Graduate School of Natural Science and Technology, Niigata University) ;
  • Ruamrungsri, Soraya (Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University)
  • 투고 : 2017.11.24
  • 심사 : 2018.05.30
  • 발행 : 2018.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

Vanda is an aerial tropical orchid native to Thailand and nitrogen (N) fertilizer is mainly used to promote its growth and quality. However, little is known about the characteristics of N absorption and assimilation in Vanda. The objective of this study was to determine the appropriate source of N for Vanda cultivation. In this experiment, shoots and roots of Vanda 'Ratchaburi Fuchs-Katsura' were sprayed weekly with 100 ml of $^{15}N$ tracer solution (1) 10 mM of $^{15}NO_3{^-}$, (2) 5 mM of $^{15}NO_3{^-}$ plus 5 mM of $NH_4{^+}$, (3) 5 mM of $NO_3{^-}$ plus 5 mM of $^{15}NH_4{^+}$ and (4) 10 mM of $^{15}NH_4{^+}$. The results indicated that plants fed with a combined N fertilizer gave the highest of $^{15}N$ use efficiency ( $^{15}NUE$) of about 21.8%, 30 days after the first feeding (DAF), compared with those fed sole sources of $^{15}NO_3{^-}$ (21.0%) and $^{15}NH_4{^+}$ (16.6%). However, a sole nitrate fertilizer or combination fertilizer did not significantly affect the total N and labelled N content. Alanine was a major amino acid found in leaves and roots at 7 DAF, whereas glutamine was mainly found in stems. At 30 DAF, tyrosine and alanine became major components in the leaves, and glutamine decreased in stems when plants were fed with a single $^{15}NH_4{^+}$ source.

키워드

과제정보

연구 과제 주관 기관 : Niigata University, Aksel T Schau Charitable Foundation

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