Journal of Apiculture (한국양봉학회지)

The Apicultural Society of Korea (한국양봉학회)
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In vitro Skin Irritation Test of Honeypolis using Human Skin Model

  • Woo, SoonOk (Sericultural & Apicultural Materials Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Han, Sangmi (Sericultural & Apicultural Materials Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Hong, Inpyo (Sericultural & Apicultural Materials Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kim, Sung-kuk (Sericultural & Apicultural Materials Division, Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
  • Received : 2018.11.14
  • Accepted : 2018.11.28
  • Published : 2018.11.30
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Abstract

Ethanol extracted propolis (EEP) was mixed with honey (honeypolis) to dissolve well in water and in vitro skin irritation test was conducted. In vitro method is designed to predict and classify the skin irritation potential of a chemical by assessment of its effect on $EpiDerm^{TM}$, a reconstituted three-dimensional human epidermis model. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after a 60 min exposure period. In this study under the given conditions honeypolis showed no irritant effects. Honeypolis meets acceptance criteria if: mean absolute OD 570 nm of the three negative control tissues is ${\geq}0.8$ and ${\leq}2.8$, mean relative tissue viability of the three positive control tissues is ${\leq}20%$, standard deviation of relative tissue viability obtained from each three concurrently tested tissues is ${\leq}18%$. Honeypolis is therefore classified as "non-irritant" in accordance with UN GHS "No Category".

Keywords

Acknowledgement

Supported by : RDA

References

  1. Bors, W., W. Heller, C. Michel and M. Saran. 1990. Flavonoids as antioxidants: determination of radicalscavenging efficiencies. Meth. Enzymol. 186: 343-355.
  2. Burdock, G.A. 1998. Review of the biological properties and toxicity of bee propolis. Food Chem. Toxicol. 36: 347-363. https://doi.org/10.1016/S0278-6915(97)00145-2
  3. Cotovio, J., M.-H. Grandidier, P. Portes, R. Roguet and G. Rubinstenn. 2005. The in vitro acute skin irritation of chemicals: optimization of the EpiSkin prediction model within the framework of the ECVAM validation process. ATLA 33: 329-349.
  4. Draize, J.H., G. Woodard and H.O. Calvery. 1944. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. Journal of Pharmacology and Experimental Therapeutics. 82: 337-390.
  5. EC. 2008. Regulation (EC) No. 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No. 1907/2006. Official Journal of the European Union L353, 1-1355.
  6. EURL-ECVAM. 2008. Statement on the scientific validity of in vitro tests for skin irritation testing, issued by the ECVAM Scientific Advisory Committee (ESAC29). 5 November 2008.
  7. EURL-ECVAM. 2009. Statement on the "Performance under UN GHS of three in vitro assays for skin irritation testing and the adaption of the Reference Chemicals and Defined Accuracy Values of the ECVAM skin irritation Performance Standards", issued by the ECVAM Scientific Advisory Committee (ESAC30). 9 April 2009.
  8. Faller, C., M. Bracher, N. Dami and R. Roguet. 2002. Predictive ability of reconstructed human epidermis equivalents for assessment of skin irritation of cosmetics. Toxicol. in vitro. 16: 557-572. https://doi.org/10.1016/S0887-2333(02)00053-X
  9. Fentem, J.H., D. Briggs, C. Chesne, G.R. Elliot, J.W. Harbell, J.R. Heylings, P. Portes, R. Roguet, J.J.M. van de Sandt and P. Botham. 2001. A prevalidation study on in vitro tests for acute skin irritation. Results and evaluation by the Management Team. Toxicol. in Vitro 15: 57-93. https://doi.org/10.1016/S0887-2333(01)00002-9
  10. Heim, K.E., A.R. Tagliaferro and D.J. Bobilya. 2002. Flavonoids antioxidants: chemistry, metabolism and structure-activity relationship. J. Nutr. Biochem. 13: 572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
  11. Kumazawa, S., H. Tomoko and N. Tsutomu. 2004. Antioxidant activity of propolis of various geographic origins. Food Chemistry 84: 329-339. https://doi.org/10.1016/S0308-8146(03)00216-4
  12. Markham, K. R., K. A. Mitchell, A. L. Wilkins, J. A. Daldy and Y. Lu. 1996. HPLC and GC-MS identification of the major organic constituents in New Zealand propolis. Phytochemistry 42: 205-211. https://doi.org/10.1016/0031-9422(96)83286-9
  13. OECD. 2014. Guidance Document on Integrated Approaches to Testing and Assessment for Skin Irritation/Corrosion, No. 203, Series on Testing and Assessment. 11 July 2014.
  14. OECD. 2015. OECD Guideline for the Testing of Chemicals No. 404: Acute Dermal Irritation/Corrosion. 28 July. 2015.
  15. Orsolic, N., J. Skuric, D. Dikic and G. Stanic. 2014. Inhibitory effect of a propolis on Di-n-Propyl Disulfide or n-Hexyl salycilate-induced skin irritation, oxidative stress and inflammatory responses in mice. FITOTERAPIA. 93: 18-30. https://doi.org/10.1016/j.fitote.2013.12.007
  16. Russo, A., R. Longo and A. Vanella. 2002. Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin, Fitoterapia. 73: S21-S29. https://doi.org/10.1016/S0367-326X(02)00187-9
  17. UN. 2015. United Nations Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Sixth revised edition. UN New York and Geneva.
  18. Woo, S.O., I.P. Hong, S.M. Han, Y.S. Choi, H.S. Sim, H.G. Kim, M.Y. Lee and M.Y. Lee. 2013. DPPH free radical scavenging effects of propolis collected in Korea, J. Apiculture. 28: 355-359.
  19. Woo, S.O., I.P. Hong and S.M. Han. 2015. Extraction properties of propolis with ethanol concentration. J. Apiculture. 30(3): 211-216. https://doi.org/10.17519/apiculture.2015.09.30.3.211
  20. Woo, S.O., S.M. Han and I.P. Hong. 2017. Properties of watersoluble propolis made with honey. J. Apiculture. 32(4): 391-394. https://doi.org/10.17519/apiculture.2017.11.32.4.391
  21. Zuang, V., M. Balls, P.A. Botham, A. Coquettte, E. Corsini, R.D. Curren, G.R. Elliot, J.H. Fentem, J.R. Heylings, M. Liebsch, J. Medina, R. Roguet, J.J.M. van de Sandt, C. Wiemann and A.P. Worth. 2002. Follow-up to the ECVAM prevalidation study on in vitro tests for acute skin irritation. ECVAM Skin irritation Task Force Report 2. ATLA. 30: 109-1029.