Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
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Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources

  • Lee, Mi Ree (College of Veterinary Medicine, Kyungpook National University) ;
  • Suh, Hye Rin (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Myeong Whan (College of Veterinary Medicine, Kyungpook National University) ;
  • Cho, Joon Young (Department of Health and Exercise Science, Korea National Sport University) ;
  • Song, Hyun Keun (Biomedical Science Institute, Changwon National University) ;
  • Jung, Young Suk (Department of Pharmacy, College of Pharmacy, Pusan National University) ;
  • Hwang, Dae Youn (College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Kil Soo (College of Veterinary Medicine, Kyungpook National University)
  • Received : 2018.10.31
  • Accepted : 2018.12.08
  • Published : 2018.12.31
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Abstract

This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, $Avertin^{(R)}$) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak $CO_2$ ($ETCO_2$), mean arterial blood pressure, heart rate, oxygen saturation ($SpO_2$), body temperature, pH, $PCO_2$, and $PO_2$ of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different strains. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, there were no significant differences in the mean arterial blood pressure, $SPO_2$, arterial blood $PCO_2$, and $PO_2$, which decreased after TBE anesthesia, or in heart rate and $ETCO_2$, which increased after TBE anesthesia. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice.

Keywords

Acknowledgement

Supported by : NLAR (National Laboratory Animal Resources)

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