Journal of Industrial and Engineering Chemistry

  • 제67권
  • /
  • Pages.231-235
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  • 2018
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  • 1226-086X(pISSN)
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  • 1876-794X(eISSN)
한국공업화학회 (The Korean Society of Industrial and Engineering Chemistry)
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Synthesis of (R,R)-2,3-butanediol from starch in a hybrid cell-free reaction system

  • Yi, Tong (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Lim, Hye Jin (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Lee, So Jeong (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Lee, Kyung-Ho (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Kim, Dong-Myung (Department of Chemical Engineering and Applied Chemistry, Chungnam National University)
  • 투고 : 2018.03.18
  • 심사 : 2018.06.27
  • 발행 : 2018.11.25
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, we demonstrate the conversion of starch to (R,R)-2,3-butanediol (2,3-BD) in a hybrid cell-free synthesis system containing a mixture of lysates derived from Escherichia coli (E. coli) and cyanobacteria. A sufficient pool of pyruvate required for the synthesis of 2,3-BD was generated by combining metabolic pathways of cyanobacteria and E. coli. Successful synthesis of 2,3-BD was achieved by additional modifications of the hybrid cell-free system with the enzymes required to convert pyruvate to 2,3-BD. The results demonstrate a new approach to harness biological pathways to expand the scope of cell-free metabolic engineering by cross-species combinations of cell lysates.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea

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  2. Cell-Free Metabolic Engineering: Recent Developments and Future Prospects vol.2, pp.2, 2019, https://doi.org/10.3390/mps2020033
  3. Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems vol.15, pp.8, 2018, https://doi.org/10.15252/msb.20198875
  4. The Evolution of Cell Free Biomanufacturing vol.8, pp.6, 2020, https://doi.org/10.3390/pr8060675
  5. Toward sustainable, cell-free biomanufacturing vol.69, pp.None, 2018, https://doi.org/10.1016/j.copbio.2020.12.012
  6. Cell-Free Biosynthesis System: Methodology and Perspective of in Vitro Efficient Platform for Pyruvate Biosynthesis and Transformation vol.10, pp.10, 2018, https://doi.org/10.1021/acssynbio.1c00252