The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)

  • Prova, Ananya (Department of Plant Pathology, EXIM Bank Agricultural University) ;
  • Akanda, Abdul Mannan (Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University) ;
  • Islam, Shaikhul (Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University) ;
  • Hossain, Md. Motaher (Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University)
  • Received : 2018.03.11
  • Accepted : 2018.07.22
  • Published : 2018.10.01
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Abstract

Stems and pods of hyacinth bean cultivated in a farmer's field in Gazipur District, Bangladesh, were found rotted in nearly 5% hyacinth bean plants. A fungus having fluffy mycelium and large sclerotia was isolated from affected tissues. Combined results of morphological, molecular and pathological analyses identified the fungus as Sclerotinia sclerotiorum (Lib) de Bary. Inoculating the fungus on healthy hyacinth bean plants and pods reproduced the symptoms previously observed in the field. The three isolates obtained from naturally infected plants were cross inoculated in hyacinth bean, okra and African-American marigold and they were pathogenic to these hosts. The optimum temperature and pH for its growth were $20^{\circ}C$ and pH 5.0, respectively. Sclerotial development was favored at pH 5.0. Sucrose and mannitol were the best carbon sources to support hyphal growth, while glucose was the most favourable for sclerotial development. The hyacinth bean genotypes, HB-82 (Rupban Sheem) and HB-102 were found highly resistant, while HB-94 (Ashina) was moderate resistant to the fungus. Finally, S. sclerotiorum was sensitive to Bavistin, Dithane M-45 and Rovral fungicides and Ca in the form of $CaCl_2$. This observation could possibly aid in eliminating field loss in hyacinth bean caused by an emerging pathogenic fungus S. sclerotiorum.

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References

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