대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)

  • 제42권5호
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  • Pages.111-118
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  • 2018
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  • 1225-1577(pISSN)
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  • 2384-0900(eISSN)
대한구강악안면병리학회 (Korean Academy of Oral and Maxillofacial Pathology)
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임플란트 표면에서 배양된 정상인 조골세포의 증식 및 분화에 미치는 니코틴의 영향

The Effect of Nicotine on the Proliferation and Differentiation of Normal Human Osteoblast at the Surface of Implants

  • 안태웅 (선 치과병원 구강악안면외과학교실) ;
  • 이종헌 (단국대학교 치과대학 구강병리학교실, 구강노화연구소)
  • Ahn, Tae Woong (Department of Oral and Maxillofacial Surgery, Sun Dental Hospital) ;
  • Lee, Chong Heon (Department of Oral Pathology, College of Dentistry, Oral Aging Research Center, Dankook University)
  • 투고 : 2018.10.02
  • 심사 : 2018.10.19
  • 발행 : 2018.10.31
⟨ 이전 논문 다음 논문 ⟩

초록

Nicotine of tobacco component has a controversial impact in the clinical outcome of dental implants. Although numerous nicotine effects on bone healing around implants have been presented, it is rarely reported in vitro study about normal human osteoblast(NHost) from oral and maxillofacial area at the surface of implants. The purpose of the present study was to evaluate the effect of nicotine on the proliferation and differentiation response of NHost to plasmatic and salivary levels of nicotine reported in smokers at the surface of screw-type plasma-sprayed titanium implants. NHosts were seeded on the surface of titanium implants and cultured for 21 days in ${\alpha}-MEM$ supplemented with 10% FBS, 50mg/ml ascorbic acid, 5mM ${\beta}$-glycerophosphate and 100nM dexamethasone. Seeded implants were exposed to various nicotine concentration(0.05-0.5mg/ml) from 1 to 21 days, and characterized for cell morphology, proliferation, differentiation, alkaline phosphatase(ALP) activity and ionized calcium concentration(Cai) of medium. Continuous exposure to higher nicotine concentration(above 0.3mg/ml) induced a dose- and time-dependent vacuolation of the cytoplasm, and a tendency to detach from the implant surface. 0.05mg/ml(lower nicotine concentration) did not cause significant effects in the cell proliferation and ALP activity. 0.1-0.2mg/ml caused evident dose-dependent effects in increased cell proliferation, ALP activity and earlier onset of matrix mineralization at levels up to 0.2mg/ml, while a dose-dependent inhibitory effect at 0.3-0.5mg/ml. Cai concentration of control group was decreased at 14 days. Increased Cai concentration at 0.1-0.2mg/ml, decreased Cai concentration at 0.3mg/ml and no change at 0.5mg/ml during the culture period were seen. It suggested that nicotine concentration could paly an role in modulating NHost activity as a contributing factor associated with proliferation and differentiation of NHost at the surface of implants.

키워드

참고문헌

  1. Albrektsson T, Berglundh T, Lindhe J: Osseointegration: Historic background and current concepts. In: Lindh J, Karring T, Niklaus PL, editors. Clinical Periodontology and Implant Dentistry, 4th ed. Oxford: Blackwell Munsksgaard. 2003. p 809-820.
  2. Davies JE: Mechanisms of endosseous integration. Int J Prosthodont. 1988;11:391-401.
  3. Leonardis D, Garg AK, Pecora GE: Osseointegration of rough acid-etched titanium implants: 5-year follow-up of 100 minimatic implants. Int J Oral Maxillofac Implants. 2006;14:384-391.
  4. DuycK J, Naert I: Failure of oral implants: Aetiology, symptoms and influencing factors. Clin Oral Invest. 1997;2:102-114.
  5. Bain CA, Moy PK: The association between the failure of dental implants and cigarette smoking. Int J Oral Maxillofac Implants. 1993;8:609-615.
  6. Haas R, Haimbock W: The relationship of smoking on periimplant tissue: A retrospective study. J Prosthet Dent. 1996;76:592-596. https://doi.org/10.1016/S0022-3913(96)90435-7
  7. Lindquist LW, Carlsson GE, Jemt T: Association between marginal bone loss around osseointegrated mandibular implants and smoking habits: A 10-year follow-up study. J Dent Res. 1997;76:1667-1674. https://doi.org/10.1177/00220345970760100801
  8. Hinode D, Tanabe S, Yamuchi E, Miyamoto Y: Influence of smoking on osseointegrated implant: A meta-analysis. Clin Oral Implants Res. 2006;17:473-478. https://doi.org/10.1111/j.1600-0501.2005.01244.x
  9. Strietzel FP, Reichart PA, Wegner B, Kuchler I: Smoking interferes with the prognosis of dental implant treatment: a systematic review and meta-analysis. J Clin Periodontol. 2007;34:523-544. https://doi.org/10.1111/j.1600-051X.2007.01083.x
  10. Chrcanovic BR, Albrektsson T, Wennerberg A: The impact of smoking on failure rates, postoperative infection and marginal bone loss of dental implants. J Dent. 2015;43:487-498. https://doi.org/10.1016/j.jdent.2015.03.003
  11. Moraschini V, PortoBarboza ED: Success of dental implants in smokers and non-smokers: a systematic review and meta-analysis. Int J Oral Maxillofac Surg. 2016;45:205-215. https://doi.org/10.1016/j.ijom.2015.08.996
  12. Jones JK, Triplett RG: The relationship of cigarette smoking to impaired intraoral wound healing: a review of evidence and implications for patient care. J Oral Maxillofac Surg. 1992;50:237-239. https://doi.org/10.1016/0278-2391(92)90318-T
  13. Meechan JG, Macgregor ID, Dennison M: The effect of smoking on immediate post-extraction socket filling with blood and on the incidence of painful socket. British J Oral Maxillofac Surg. 1988;26:402-409. https://doi.org/10.1016/0266-4356(88)90093-9
  14. Holm G: Smoking as an additional risk for tooth loss. J Periodontol. 1994;65:996-1001. https://doi.org/10.1902/jop.1994.65.11.996
  15. Carlsson GE, Torsten J, Lindquist LW: Long term marginal periimplant bone loss in edentulous patients. Inter J Prosthodont. 2000;13:295-302.
  16. Rivera-Hidalgo F: Smoking and periodontal disease. Periodontol. 2003;32:50-58. https://doi.org/10.1046/j.0906-6713.2003.03205.x
  17. Nociti FHJ, Neto CJB, Sallum EA. Intermittent cigarette smoke inhalation may affect bone volume around titanium implants in rats. J Periodontol 2002;73:982-987. https://doi.org/10.1902/jop.2002.73.9.982
  18. Cesar-Neto JB, Duarte PM, Nociti FH Jr. A comparative study on the effect of nicotine administration and cigarette smoke inhalation on bone healing around titanium implants. J Periodontol. 2003;74:1445-1459.
  19. Cesar-Neto JB, Sallum EA, Nociti FH Jr. Bone density around titanium implants may benefit from smoking cessation: A histological study in rats. Int J Oral Maxillofac Implants. 2005;20:713-719.
  20. Russell MA, Iyer R, Feyerabend C. Relation of nicotine yield of cigarettes to blood nicotine concentrations in smokers. BMJ. 1980;280:972-976. https://doi.org/10.1136/bmj.280.6219.972
  21. Gritz ER, Van Vunakis H, Jarvik ME: Plasma nicotine and cotinine concentrations in habitual smokeless tobacco users. Clin Pharmacol Ther. 1981;30:201-209. https://doi.org/10.1038/clpt.1981.149
  22. Hoffmann D, Adams JD: Carcinogenic tobacco-specific Nnitrosamines in snuff and in the saliva of snuff dippers. Cancer Res. 1981;41:4305-308.
  23. Hill P, Haley NJ, Wynder EL: Cigarrete smoking: Carboxyhemoglobin, plasma nicotine, cotinine and thiocyanate vs self reported smoking data and cardiovascular disease. J Chron Dis. 1983;36:439-449. https://doi.org/10.1016/0021-9681(83)90136-4
  24. Ryder MI, Hamada M, Hyun W: Alterations of neutrophil L-selection and CD18 expression by tobacco smoke: implications for periodontal diseases. J Periodontol Res. 1998;33:359-369. https://doi.org/10.1111/j.1600-0765.1998.tb02211.x
  25. Cuff MJ, MacQuade MJ, Van Dyke TE: The presence of nicotine on root surfaces of periodontally diseased teeth in smokers. J Periodontol. 1989;60:564-569. https://doi.org/10.1902/jop.1989.60.10.564
  26. Daftari KT, Whitesides TE, Heller JG, Hutton CW: Nicotine on the revascularization of bone graft. An experimental study in rabbits. Spine. 1994;19:904-911. https://doi.org/10.1097/00007632-199404150-00007
  27. Hollinger OJ, Schmitt JM, Buck D, Hwang K: Impact of nicotine on bone healing. J Biomed Mater Res. 1999;45:294-301. https://doi.org/10.1002/(SICI)1097-4636(19990615)45:4<294::AID-JBM3>3.0.CO;2-1
  28. Park GJ, Lee CH: Primary Culture of Normal Human Osteoblast from Oral and Maxillofacial Area. Kor J Oral Maxillofac Pathol. 2009;33: 259-266.
  29. Oh MG, Park GJ, Lee CH: The Effect of BMP-2 on Proliferation and Differentiation of Cultured Normal Human Osteoblast Cell. Kor J Oral Maxillofac Pathol. 2012;36:263-268.
  30. Stein G, Lian J: Molecular mechanisms mediated proliferation-Differentiation interrelationships during progressive development of the osteoblast phenotype: Update. End Rev. 1995;4:290-297.
  31. Coelho MJ, Fernandes MH: Human bone cells cultures in biocompatibility testing. Part II: Effect of ascorbic acid, b-Glicerophosphate and dexamehasone on osteoblast differentiation. Biomaterials. 2000;21:1095-1102. https://doi.org/10.1016/S0142-9612(99)00192-1
  32. Jrgensen NR, Henriksen Z, Civitelli R: Dexamethasone, BMP-2, and 1,25-dihydroxyvitamin D enhance a more differentiated osteoblast phenotype: validation of an in vitro model for human bone marrowderived primary osteoblasts. Steroids. 2004;69:219-226. https://doi.org/10.1016/j.steroids.2003.12.005
  33. Balatsouka D, Gotfredsen K, Berglundh T: The impact of nicotine on osseointegration. An experimental study in the femur and tibia of rabbits. Clin Oral Implants Res. 2005;16:389-395. https://doi.org/10.1111/j.1600-0501.2004.01151.x
  34. Ramp WK, Lenz LG, Galvin RJ: Nicotine inhibits collagen synthesis and alkaline phosphatase activity, but stimulates DNA synthesis in osteoblast-like cells. Proc Soc Exp Biol Med. 1991;197:36-33. https://doi.org/10.3181/00379727-197-43221
  35. De Duve C, De Barsy T, Van Hoof F: Commentary: Lysosomotropic drugs. Biochem Pharmacol. 1974;23:2495-2531. https://doi.org/10.1016/0006-2952(74)90174-9
  36. Lynch M, Stein J, Stein G, Lian J: The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: Modification of expression of genes supporting cell growth, adhesion and extracellular matrix mineralization. Exp Cell Res. 1995;216:35-34. https://doi.org/10.1006/excr.1995.1005
  37. Bellows CG, Aubin JE, Heersch JNM: Initiation and progression of mineralization of bone nodules formed in vitro: The role of alkaline phosphatase and organic phosphates. Bone Miner. 1991;14:27-30. https://doi.org/10.1016/0169-6009(91)90100-E
  38. Bellows CG, Heersche JNM, Aubin JE: Inorganic phosphate added exogenously or released from b-glycerophosphate iniciates mineralization of osteoid in vitro. Bone and Mineral. 1992;17:15-29. https://doi.org/10.1016/0169-6009(92)90707-K
  39. Anderson HC, Morris DC: Mineralization. In: Mundy JR, Martin TJ, eds. Handbook of Experimental Pharmacology, Vol. 107, 267, 1993 Berlin: Springer-Verlag.
  40. Pereira ML, Carvalho JC, Fernandes MH: Behaviour of human osteoblastic cells cultured on plasma-sprayed titanium implants in the presence of nicotine. Clin Oral Impl Res. 2008;19:582-589. https://doi.org/10.1111/j.1600-0501.2007.01515.x
  41. Yuhara S, Hirose S, Hagiwara H: Effects of nicotine on cultured cells suggest that it can influence the formation and resorption of bone. Eur J Pharmacol. 1999;383:387-393. https://doi.org/10.1016/S0014-2999(99)00551-8
  42. Walker LM, Preston MR, El Hay AJ: Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture. Bone. 2001;28:603-608. https://doi.org/10.1016/S8756-3282(01)00427-6
  43. Kosaka N, Uchu M: Effect of benidipine hydrochloride, a dihydropyridine-type calcium antagonist, on the function of mouse osteoblastic cells. Calcif Tissue Int. 1998;62:554-556. https://doi.org/10.1007/s002239900478
  44. Eklon-Kalonji E, Denis I, Lieberherr M, Pointillart A: Effects of extracellular calcium on the proliferation and differentiation of porcine osteoblasts in vitro. Cell Tissue Res. 1998;292:163-171. https://doi.org/10.1007/s004410051046
  45. Lambert PM, Morris HF, Ochi S: The influence of smoking on 3-year clinical success of osseointegrated dental implants. Annals Periodontol. 2000;5:79-89. https://doi.org/10.1902/annals.2000.5.1.79
  46. Dvorak M, Siddiqua A, Dallas SL, Riccardi D: Physiological changes in extracellular calcium concentration directly control osteoblast function in the absence of calciotropic hormones. PNAS USA. 2004;101: 5140-5145. https://doi.org/10.1073/pnas.0306141101
  47. Ikeda H, Shiraiwa M, Tanaka T: Difference in penetration of horseradish peroxidase tracer as a foreign substance into the peri-implant or junctional epithelium of rat gingivae. Clin Oral Implants Res. 2002;13:243-251. https://doi.org/10.1034/j.1600-0501.2002.130303.x