Quantitative Bio-Science

Institute of Natural Science, College of Natural Sciences, Keimyung University (계명대학교 자연과학연구소)
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Isolation and Identification of Noble Lactic Acid Bacteria

  • Yeo, Han-Cheol (Department of Biological Sciences, Keimyung University) ;
  • Jang, Jin-Young (Department of Biological Sciences, Keimyung University) ;
  • Park, Hyeong-Jun (Department of Biological Sciences, Keimyung University) ;
  • Min, Byung-Tae (Department of Biological Sciences, Keimyung University) ;
  • Yoo, Min (Department of Biological Sciences, Keimyung University)
  • Received : 2018.10.25
  • Accepted : 2018.11.19
  • Published : 2018.11.30
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Abstract

In this study, noble strains of lactic acid bacteria were isolated and identified by genetic analysis of 16s rRNA. Also, pH-dependent growth curve, cholesterol assimilation ability and sugar production efficiency were measured. Lactic acid bacteria were identified to inhabit in the milks from various animals. Results of sequence analysis showed that there were differences in 16S rRNA sequence among strains and part of gene deletion was also recognized. Growth rates were varied, too, depending on the pH of the medium. Lactobacillus rhamnosus LOCK908 isolated from cow milk showed the highest growth rate and high cholesterol assimilation ability. Results of sugar fermentation tests were relatively consistent with the sequencing results. So, we propose newly isolated Lactobacillus rhamnosus LOCK908 as useful candidate for a starter of fermented beverage and probiotics. Results of this study will contribute to the isolation and identification of noble Lactic acid bacteria and to the public health.

Keywords

Acknowledgement

Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET)

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