Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
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Analysis of Varietal Difference and Genetic Diversity of Grapevine Cultivarsthrough the Leaf Inoculation of Colletotrichum spp.

포도 탄저병균의 엽면접종을 통한 국내 육성 포도나무의 품종 간 저항성 검정 및 유전적 다양성 분석

  • Jang, Hyun A (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Lee, Kyo-Sang (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Oo, May Moe (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Kwak, Tae-Seok (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Yoon, Ha-Yeon (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Thinn, Khaing Shwe Zin (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Kim, Mi-Reu (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Kim, Dae-Gyu (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Lee, Jeong Jin (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Lim, Gi Taek (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University) ;
  • Hur, Youn Young (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Oh, Sang-Keun (Department of Applied Biology, College of Agriculture & Life Sciences, Chungnam National University)
  • 장현아 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 이교상 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 모우메이 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 곽태석 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 윤하연 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 카잉 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김미르 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김대규 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 이정진 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 임기택 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 허윤영 (농촌진흥청 국립원예특작과학원) ;
  • 오상근 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2018.10.23
  • Accepted : 2018.11.13
  • Published : 2018.12.31
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Abstract

Here, we reported 159 varieties of major cultivars using grapevine genetic resources to identify the resistant grape ripe rot cultivars. To do this, we performed pathogenicity assays from these grape cultivars by inoculating Colletotrichum acutatum and Colletotrichum gloeosporioides. Genotyping-by-sequencing(GBS) method was also used to compare genetic diversity among grape varieties. As a result, leaves inoculated with C. acutatum showed that 58 cultivars were susceptible, while 17 cultivars were resistant. In the case of C. gloeosporioides, 34 cultivars were found to be susceptible, while 25 cultivars were resistant. The 8 cultivars that showed resistance to both species were 'Agawan', 'Huangguan', 'Xiangfei', and 5 other cultivars from the hybrids of European and American species. Most of the varieties such as 'Emerald Seedless', 'Tano Red', and 'Rem 46-77(Aestivalis GVIT 0970)' originated in European species were identified as susceptible. These results can be used in the effective management of grape disease. In addition, these findings provide information for the development and cultivation of resistant to grape ripe rot disease cultivars.

본 연구는 품종 간 변이의 폭이 좁은 포도속(Vitis spp.) 유전자원을 대상으로 탄저병 저항성 품종을 판별하기 위해 국내 주요 재배 품종 및 유전자원 159품종을 공시하고,Colletotrichum acutatum과 C. gloeosporioides를 엽면접종하여 병원성 검정을 수행하였다. 또한, Genotyping-by-sequencing(GBS) 방법을 이용하여 포도나무 품종 간 유전적 다양성을 비교 분석하였다. 그 결과, C. acutatum을 접종한 잎에서는 58품종은 감수성을, 17품종은 저항성을 나타내었고, C. gloeosporioides를 접종한 경우에는 34품종이 감수성을, 25품종은 저항성을 나타냈다. 두 균주에 대해 공통적으로 저항성을 보인 품종은 'Agawan', 'Huangguan', 'Xiangfei' 등 8품종이였으며, 이들 대부분은 유럽종과 미국종의 교잡종으로 분류되었다. 유럽종에 기원한 'Emerald Seedless', 'Tano Red', 'Rem 46-77(Aestivalis GVIT 0970)' 등의 품종들은 대부분 감수성으로 판별되었다. 따라서 본 연구 결과를 통해 확보된 병 검정 및 유전 분석 결과는 포도나무 병해를 효과적으로 관리하는데 유용하게 활용될 수 있으며, 특히 포도나무 탄저병 저항성 품종들을 개발하고 육성하는데 매우 중요한 육종 소재 및 정보가 될 것으로 사료된다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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