ANNALS OF ANIMAL RESOURCE SCIENCES (동물자원연구)

INSTITUTE OF ANIMAL RESOURCES (강원대학교 동물자원공동연구소)
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Association Study of Zygote Arrest 1 on Semen Kinematic Characteristics in Duroc Boars

두록 정자 운동학적 특성과 Zygote arrest 1 유전자 변이와의 연관성 분석

  • Lee, Mi Jin (National Institute of Animal Science, RDA) ;
  • Ko, Jun Ho (National Institute of Animal Science, RDA) ;
  • Kim, Yong Min (National Institute of Animal Science, RDA) ;
  • Choi, Tae Jeong (National Institute of Animal Science, RDA) ;
  • Cho, Kyu Ho (National Institute of Animal Science, RDA) ;
  • Kim, Young Sin (National Institute of Animal Science, RDA) ;
  • Jin, Dong Il (Department of Animal Science & Biotechnology, Chungnam National University) ;
  • Kim, Nam Hyung (Department of Animal Science, Chungbuk National University) ;
  • Cho, Eun Seok (National Institute of Animal Science, RDA)
  • 이미진 (농촌진흥청 국립축산과학원 양돈과) ;
  • 고준호 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김용민 (농촌진흥청 국립축산과학원 양돈과) ;
  • 최태정 (농촌진흥청 국립축산과학원 양돈과) ;
  • 조규호 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김영신 (농촌진흥청 국립축산과학원 양돈과) ;
  • 진동일 (충남대학교 농업생명과학대학) ;
  • 김남형 (충북대학교 축산학과) ;
  • 조은석 (농촌진흥청 국립축산과학원 양돈과)
  • Received : 2018.05.09
  • Accepted : 2018.10.22
  • Published : 2018.12.31
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Abstract

The Zygote arrest 1 (ZAR1) gene is known to affect early embryonic development in various vertebrates. In this study, we performed the association analysis to check whether there is any significant relationship between semen kinematic characteristics and the ZAR1 gene. To determine semen kinematic characteristics, we measured motility (MOT), straight-line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), linearity (LIN), straightness (STR), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF) of spermatozoa in boars. In order to detect single nucleotide polymorphisms (SNPs), we extracted genomic DNA from multiple Duroc boars, and then subsequently used them in sequencing reactions. As a result, three SNPs were detected in the intronic region of ZAR1 gene (g.2435T>C in intron 2, g.2605G>A and g.4633A>C in intron 3 ). SNPs g.2435T>C and g.2605G>A were significantly associated with MOT (p<0.01) and VSL (p<0.05), and g.4633A

ZAR1은 척추동물의 초기 배아 발달에 영향을 미치는 유전자로 알려져 있다. 본 연구는 두록 수퇘지 112두에서 ZAR1 유전자의 SNP을 발굴하고 ZAR1 유전자의 인트론 Single nucleotide polymorphism(SNP)과 정액의 운동학적 특성들과의 연관성을 규명하였다. 돼지의 정액 운동학적 특성을 분석하기 위해서 운동성(motility, MOT), 직선운동 속도(straight-line velocity, VSL), 곡선운동 속도(curvilinear velocity, VCL), 평균운동 속도(average path velocity, VAP), 직진성(linearity, LIN), 선형성(straightness, STR), 측두 이동거리(amplitude of lateral head displacement, ALH) 및 머리 진동수(beat cross frequency, BCF)를 측정하였다. SNP을 탐색하기 위해서 돼지의 혈액에서 genomic DNA를 추출하여 PCR(polymerase chain reaction)을 통해 시퀀싱 분석을 하여 유전자형을 분석하였다. 그 결과 ZAR1의 non-coding 영역인 인트론에서 SNP 3개를 확인했으며 각 각 인트론 2 영역에서 g.2435T>C, 인트론3 영역에서 g.2605G>A, g.4633A>C 변이를 보였다. g.2435T>C, g.2605G>A는 각 각 MOT(p<0.01)와 VSL(p< 0.05)에서 높은 연관성을 나타냈고 g.4633A>C는 MOT, VCL, VSL, VAP, ALH에서 높은 연관성을 나타냈다(p<0.01). 본 연구에서는 ZAR1 유전자의 SNP이 돼지 정액의 운동학적 특성들에 영향을 미치는 것으로 나타났다. 이러한 결과는 ZAR1이 우수한 수퇘지들에서 번식능력이 높은 개체를 선발할 수 있는 후보유전자로 제시하며 다양한 돼지의 품종과 더 많은 두수를 확보하여 검증연구를 통해 우수한 능력의 수퇘지에서 활력이 좋은 개체를 선발하는 분자마커 연구의 기초자료로 사용이 가능하다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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