대한두개안면성형외과학회지 (Archives of Craniofacial Surgery)

  • 제19권3호
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  • Pages.181-189
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  • 2018
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  • 2287-1152(pISSN)
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  • 2287-5603(eISSN)
대한두개안면성형외과학회 (Korean Cleft Palate-Craniofacial Association)
권호 표지
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Effects of three-dimensionally printed polycaprolactone/β-tricalcium phosphate scaffold on osteogenic differentiation of adipose tissue- and bone marrow-derived stem cells

  • Park, Hannara (Department of Plastic and Reconstructive Surgery, Daegu Fatima Hospital) ;
  • Kim, Jin Soo (Department of Plastic and Reconstructive Surgery, Daegu Fatima Hospital) ;
  • Oh, Eun Jung (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Kim, Tae Jung (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Kim, Hyun Mi (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Shim, Jin Hyung (Department of Mechanical Engineering, Korea Polytechnic University) ;
  • Yoon, Won Soo (Department of Mechanical Engineering, Korea Polytechnic University) ;
  • Huh, Jung Bo (Department of Prosthodontics, Dental Research Institute, Institute of Translational Dental Science, School of Dentistry, Pusan National University) ;
  • Moon, Sung Hwan (Department of Medicine, Konkuk University School of Medicine) ;
  • Kang, Seong Soo (College of Veterinary Medicine, Chonnam National University) ;
  • Chung, Ho Yun (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University)
  • 투고 : 2018.04.16
  • 심사 : 2018.09.10
  • 발행 : 2018.09.20
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초록

Background: Autogenous bone grafts have several limitations including donor-site problems and insufficient bone volume. To address these limitations, research on bone regeneration is being conducted actively. In this study, we investigate the effects of a three-dimensionally (3D) printed polycaprolactone (PCL)/tricalcium phosphate (TCP) scaffold on the osteogenic differentiation potential of adipose tissue-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMSCs). Methods: We investigated the extent of osteogenic differentiation on the first and tenth day and fourth week after cell culture. Cytotoxicity of the 3D printed $PCL/{\beta}-TCP$ scaffold was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay, prior to osteogenic differentiation analysis. ADSCs and BMSCs were divided into three groups: C, only cultured cells; M, cells cultured in the 3D printed $PCL/{\beta}-TCP$ scaffold; D, cells cultured in the 3D printed $PCL/{\beta}-TCP$ scaffold with a bone differentiation medium. Alkaline phosphatase (ALP) activity assay, von Kossa staining, reverse transcription-polymerase chain reaction (RT-PCR), and Western blotting were performed for comparative analysis. Results: ALP assay and von Kossa staining revealed that group M had higher levels of osteogenic differentiation compared to group C. RT-PCR showed that gene expression was higher in group M than in group C, indicating that, compared to group C, osteogenic differentiation was more extensive in group M. Expression levels of proteins involved in ossification were higher in group M, as per the Western blotting results. Conclusion: Osteogenic differentiation was increased in mesenchymal stromal cells (MSCs) cultured in the 3D printed PCL/TCP scaffold compared to the control group. Osteogenic differentiation activity of MSCs cultured in the 3D printed PCL/TCP scaffold was lower than that of cells cultured on the scaffold in bone differentiation medium. Collectively, these results indicate that the 3D printed PCL/TCP scaffold promoted osteogenic differentiation of MSCs and may be widely used for bone tissue engineering.

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참고문헌

  1. Liao HT, Lee MY, Tsai WW, Wang HC, Lu WC. Osteogenesis of adipose-derived stem cells on polycaprolactone-$\beta$-tricalcium phosphate scaffold fabricated via selective laser sintering and surface coating with collagen type I. J Tissue Eng Regen Med 2016;10:E337-53. https://doi.org/10.1002/term.1811
  2. Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol 2004;36:568-84. https://doi.org/10.1016/j.biocel.2003.11.001
  3. Rose FR, Cyster LA, Grant DM, Scotchford CA, Howdle SM, Shakesheff KM. In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel. Biomaterials 2004;25:5507-14. https://doi.org/10.1016/j.biomaterials.2004.01.012
  4. Sachlos E, Czernuszka JT. Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. Eur Cell Mater 2003;5:29-39. https://doi.org/10.22203/eCM.v005a03
  5. Arafat MT, Lam CX, Ekaputra AK, Wong SY, Li X, Gibson I. Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering. Acta Biomater 2011;7:809-20. https://doi.org/10.1016/j.actbio.2010.09.010
  6. Xue R, Qian Y, Li L, Yao G, Yang L, Sun Y. Polycaprolactone nanofiber scaffold enhances the osteogenic differentiation potency of various human tissue-derived mesenchymal stem cells. Stem Cell Res Ther 2017;8:148. https://doi.org/10.1186/s13287-017-0588-0
  7. Matsuno T, Nakamura T, Kuremoto K, Notazawa S, Nakahara T, Hashimoto Y, et al. Development of beta-tricalcium phosphate/collagen sponge composite for bone regeneration. Dent Mater J 2006;25:138-44. https://doi.org/10.4012/dmj.25.138
  8. Choi D, Kumta PN. Mechano-chemical synthesis and characterization of nanostructured $\beta$-TCP powder. Mater Sci Eng C 2007;27:377-81. https://doi.org/10.1016/j.msec.2006.05.035
  9. Minoda R, Hayashida M, Masuda M, Yumoto E. Preliminary experience with beta-tricalcium phosphate for use in mastoid cavity obliteration after mastoidectomy. Otol Neurotol 2007;28: 1018-21. https://doi.org/10.1097/MAO.0b013e3181557b7c
  10. Burg KJ, Porter S, Kellam JF. Biomaterial developments for bone tissue engineering. Biomaterials 2000;21:2347-59. https://doi.org/10.1016/S0142-9612(00)00102-2
  11. Yang S, Leong KF, Du Z, Chua CK. The design of scaffolds for use in tissue engineering. Part I: traditional factors. Tissue Eng 2001;7:679-89. https://doi.org/10.1089/107632701753337645
  12. Hutmacher DW, Sittinger M, Risbud MV. Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. Trends Biotechnol 2004;22:354-62. https://doi.org/10.1016/j.tibtech.2004.05.005
  13. Yang S, Leong KF, Du Z, Chua CK. The design of scaffolds for use in tissue engineering. Part II: rapid prototyping techniques. Tissue Eng 2002;8:1-11. https://doi.org/10.1089/107632702753503009
  14. Guan J, Zhang J, Li H, Zhu Z, Guo S, Niu X, et al. Human urine derived stem cells in combination with $\beta$-TCP can be applied for bone regeneration. PLoS One 2015;10:e0125253. https://doi.org/10.1371/journal.pone.0125253
  15. Carvalho PP, Leonor IB, Smith BJ, Dias IR, Reis RL, Gimble JM, et al. Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration: nude mice calvarial defect in vivo study. J Biomed Mater Res A 2014;102:3102-11. https://doi.org/10.1002/jbm.a.34983
  16. Middleton JC, Tipton AJ. Synthetic biodegradable polymers as orthopedic devices. Biomaterials 2000;21:2335-46. https://doi.org/10.1016/S0142-9612(00)00101-0
  17. Kim SJ, Kim MR, Oh JS, Han I, Shin SW. Effects of polycaprolactone-tricalcium phosphate, recombinant human bone morphogenetic protein-2 and dog mesenchymal stem cells on bone formation: pilot study in dogs. Yonsei Med J 2009;50:825-31. https://doi.org/10.3349/ymj.2009.50.6.825

피인용 문헌

  1. Three-dimensional TCP scaffolds enriched with Erythropoietin for stimulation of vascularization and bone formation vol.16, pp.2, 2018, https://doi.org/10.29333/ejgm/108620
  2. Three-Dimensional Printing for Craniofacial Bone Tissue Engineering vol.26, pp.23, 2018, https://doi.org/10.1089/ten.tea.2020.0186
  3. CTP‐CM enhances osteogenic differentiation of hPDLSCs via NF‐κB pathway vol.27, pp.3, 2018, https://doi.org/10.1111/odi.13567
  4. Impact of 3D cell culture on bone regeneration potential of mesenchymal stromal cells vol.12, pp.1, 2018, https://doi.org/10.1186/s13287-020-02094-8