Journal of Chitin and Chitosan (한국키틴키토산학회지)

The Korean Society for Chitin and Chitosan (한국키틴키토산학회)
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Studies on the Sugar Analysis and Biological Activity of Sap from Juglans mandshurica Maxim.

가래나무 수액의 당성분 분석 및 생리활성 연구

  • Kim, Dae-Wook (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Choi, Mi-Na (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Lee, Min-Sung (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Jung, Hae-Suk (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Byeon, Jun-Gi (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Kim, Yeong-Su (Forest Plant Industry Department, Baekdudaegan National Arboretum)
  • 김대욱 (국립백두대간수목원 산림식물산업부) ;
  • 최미나 (국립백두대간수목원 산림식물산업부) ;
  • 이민성 (국립백두대간수목원 산림식물산업부) ;
  • 정회석 (국립백두대간수목원 산림식물산업부) ;
  • 변준기 (국립백두대간수목원 산림식물산업부) ;
  • 김영수 (국립백두대간수목원 산림식물산업부)
  • Received : 2018.11.30
  • Accepted : 2018.12.12
  • Published : 2018.12.30
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Abstract

Even though the saps have been consumed as beverage in Korea, however a little research has been conducted on the chemical composition. We determined free sugars, mineral in the sap from Juglans mandshurica Maxim. collected from BongHwa region. The contents of glucose, sucrose, and fructose in the sap from J. mandshurica were 0.15, 2.73, and 0.09%, respectively. The prominent minerals in the sap from J. mandshurica were calcium and potassium. The contents of Ca, K, Mg, Fe, and Mn of sap from J. mandshurica were 0.61, 0.57, 0.12, 0.002, and 0.014 ppm, respectively. The sap from J. mandshurica show inhibitory effect on elastase. And real-time RT-PCR showed that sap from J. mandshurica increased mRNA level of AQP3 and HAS2 gene and increased hyaluronic acid production in HaCaT cells. These results indicated that the sap from J. mandshurica can potentially be used for developing cosmetic ingredient for skin moisturizing and anti-aging.

우리나라토종호두나무인가래나무(Juglans mandshurica Maxim.)의 수액의 유리당 함량은 약 3%로 다른 나무 수액에 비해 높게 나타났으며 미량 원소 중에는 Ca이 30 mg/kg의 농도로 포함되어 있는 것으로 확인되었다. 가래나무 수액 $200{\mu}g/ml$에서 elastase 저해 활성이 약 30% 정도를 보이고 있고 HaCaT 세포를 이용한 피부보습 효과에서는 자외선을 조사한 HaCaT세포의 aquaporin 3 유전자와 hyaluronan synthase 2 유전자 발현을 촉진시켜 hyaluronic acid의 생합성을 증가시킨 것으로 확인되었다. 본 연구를 통해 가래나무 수액의 피부 노화 방지 효능을 최초로 규명하였고 이는 가래나무 수액의 활용도를 높일 수 있는 가능성을 제시한 결과라 사료된다. 다만 물질 분석과 in-vivo 시험, 임상 연구 등 심도 있는 추가 연구가 필요할 것으로 판단된다.

Keywords

Acknowledgement

Grant : 봉화군 유용생물자원 발굴조사

Supported by : 경상북도 봉화

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