한국키틴키토산학회지 (Journal of Chitin and Chitosan)

한국키틴키토산학회 (The Korean Society for Chitin and Chitosan)
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천연항균제를 사용한 냉장떡류의 유통 안정성 평가연구

A Study on the Evaluation of Distribution Stability of Refrigerated Rice Cake Using Natural Antibiotics

  • 투고 : 2018.11.29
  • 심사 : 2018.12.24
  • 발행 : 2018.12.30
⟨ 이전 논문 다음 논문 ⟩

초록

냉장유통을 목적으로 하는 떡류의 저장안전성을 높이기 위하여, 1차로 공정의 변화, 2차로 천연항균성 물질을 사용하여 미생물을 억제하고자 하였다. 사용한 떡류로는 녹두깨찰떡과 통팥찰떡을 사용하였고, 천연항균제로서는 자몽종자추출물, 포도씨추출물, 복합황금추출물, polylysine, chitosan, ethylpyrubate 및 glycine을 사용하였다. 액상의 천연추출물은 0, 0.25, 0.5, 1, 2, 4% 농도까지 조정하여 미생물 변화를 확인하였고 냉장온도를 위해 4, 8, $12^{\circ}C$ 측정하였다. 자몽추출물의 경우 $4^{\circ}C$ 저장에서 모든 농도에서 미생물 안전성이 충분히 유지되는 것을 확인하였으나 냉장온도 중상온에 해당하는 $8^{\circ}C$, 21일 저장에서 이미 $10^5cfu/g$를 넘어 기준에 적합하지 않은 것을 확인하였고, $12^{\circ}C$ 저장에서는 자몽추출물의 농도가 2%에서 급격히 세균수가 안정화를 이루는 것으로 보여 이 농도 또한 미생물 항균활성이 유지되는 것을 확인하였다. 포도씨추출물의 경우 $8^{\circ}C$ 배양에서 4%에서만 최종 21일 배양에서 식품유통 안전기한의 요구조건을 충족하였고, $12^{\circ}C$에서는 4% 농도에서 18일까지만 안전기준을 만족하였다. 복합황금추출물의 농도별 처리에 따른 일반 총균수를 보여주고 있는데 저온($4^{\circ}C$)에서 안정적으로 미생물의 유통안전이 유지되는 것을 확인할 수 있었으나, $8^{\circ}C$, $12^{\circ}C$에서는 다른 처리 시료와 동일하게 유통한 계치를 넘었다. Polylysine을 녹두깨 찰떡에 적용한 경우 $4^{\circ}C$에서 무첨가군을 제외한 모든 군에서 활성을 나타내는 경향을 보여주고 있으나 $8^{\circ}C$와 $12^{\circ}C$에서는 그러한 특성들이 보이지 않았다. Cchitosan의 농도 0.5% 이상에서는 매우 안정적으로 미생물의 생육이 억제되는 것으로 보여지고, 8, $12^{\circ}C$에서도 유사한 경향성이 보이고 있어 미생물을 억제하는 항균성 소재로서 가능성이 있을 것으로 보이나, $12^{\circ}C$에서는 모두 일반세균수에 대한 유통기준을 상회하였다. Ethyl-pyruvate는 $4^{\circ}C$, 1% 농도에서 미생물 안전이 유지되는 것을 보이고 있고 이후의 2, 4%에서도 식품으로서의 안전이 안정적으로 유지되는 것을 알 수 있다. Glycine을 0.25~4%까지 농도를 달리하여 4, 8, $12^{\circ}C$의 냉장조건에서 성장억제 현상을 관찰하였는데 저온인 $4^{\circ}C$에서는 매우 우수하게 효과적으로 유통안전성이 유지되는 것을 확인하였다. 그러나 $8^{\circ}C$, $12^{\circ}C$에서는 다른 항균활성물질의 첨가와 마찬가지로 14일의 유통기한을 유지하지 못하였다.

Natural extract in liquid phase was adjusted to 0, 0.25, 0.5, 1, 2, and 4% concentration to check microbial changes and to measure 4, 8, $12^{\circ}C$ for refrigeration temperature. In the case of grapefruit extract, the microbial safety was maintained at all the concentrations at $4^{\circ}C$ storage, but the antimicrobial activity was maintained at $12^{\circ}C$ storage and at $8^{\circ}C$ and 21 days storage. In the case of grape seed extract, only the 4% of the culture at $8^{\circ}C$ satisfied the requirement of safety of food distribution for the last 21 days, and the safety criterion was satisfied only at 4% concentration at $12^{\circ}C$ for 18 days. Complex Scutellaria baicalensis extract showed the total number of microbial cells treated by concentration. It was confirmed that microbial flow safety was maintained at low temperature ($4^{\circ}C$). However, at $8^{\circ}C$ and $12^{\circ}C$, Exceeded the distribution limit. When polylysine was applied to brown rice cake, it showed activity in all groups except $4^{\circ}C$, but these properties were not observed at $8^{\circ}C$ and $12^{\circ}C$. At a concentration of 0.5% or more of chitosan, the growth of the microorganism is suppressed by the 21st day very stably, and a similar tendency is observed at 8 and $12^{\circ}C$, so that it may be an antimicrobial material that inhibits microorganisms. At the first day, the distribution standards for general bacterial counts were exceeded.Ethyl-pyruvate showed that microorganism safety was maintained at $4^{\circ}C$ and 1% concentration, and food safety was stable even at 2 or 4%. Glycine showed very good and stable distribution stability at $4^{\circ}C$. However, at $8^{\circ}C$ and $12^{\circ}C$, the shelf life of 14 days could not be maintained as with the addition of other antimicrobial active substances.

키워드

과제정보

연구 과제 주관 기관 : 한국산업기술진흥원

참고문헌

  1. 식품안전정보포털, 식중독통계. https://www.foodsafetykorea. go.kr/portal/healthyfoodlife/foodPoisoningStat.do
  2. Sibel, R.: Natural antimicrobials for the minimal processing of foods. Sibel, R. (Ed.). Woodhead Publishing Limited, Cambridge, UK. (2003)
  3. Davidson, P. M. and Post, L. S.: Naturally occurring and miscellaneous food antimicrobials. In Antimicobials in foods. Branen, A. L. and Davidson, P. M. (Eds.), Marcel Dekker, Inc., New York. p. 371, 1983.
  4. Lewis, R. J.: Their regulatory status their use by the food industry. In Food additives handbook, Robert, W. D. (Ed.), Nostrand Reinhold, New York. pp. 3-27, 1989.
  5. Lee, Y. C., Oh, S. W., and Hong, H. D.: Antimicrobial characteristics of edible medicinal herbs extracts. Korean. J. Food Sci. Technol. 2002, 34, 700-709.
  6. Kim, Y. S. and Shin, D. H.: Researches on the volatile antimicrobial compounds from edible plants and their food application. Korean J. Food Sci. Technol. 2003, 35, 159-165.
  7. Ahn, Y. S. and Shin, D. H.: Antimicrobial effects of orgnic acids and ethanol on servral foodbone micororganisms. Korean J. Food Sci. Technol. 1999, 31, 1315-1323.
  8. Sebastien, F. and Robert, W. E.: Lactoferrin-a multifunctional protein with antimicrobial properties. Molecular Immunology 2003, 40, 395-405. https://doi.org/10.1016/S0161-5890(03)00152-4
  9. Naidu, A. S.: Immobilized lactoferrin antimicrobial agents and use. U. S. patent 6,172,040 B1, 2001.
  10. Branen, J. K. and Davidson, P. M.: Enhancement of nisin, lysozyme, and monolaurin antimicrobial activities by ethylenediaminetetraacetic acid and lactoferrin. J. Food Microbiol. 2004, 90, 63-74. https://doi.org/10.1016/S0168-1605(03)00172-7
  11. Steve, T., Jeremy, B., Claire, K., Ted, B., and Mary, M.: Safety determination for the use of bovine milk-derived lactoferrin as a component of an antimicrobial beef carcass spray. Regulatory Toxicology and Pharmacology 2004, 39, 12-24. https://doi.org/10.1016/j.yrtph.2003.10.001
  12. Losso, J. N., Nakai, S., and Charter, E. A.: Lysozyme. In Natural food antimicrobial system, Naidu, A. S. (ed.). 2000, pp. 17-102.
  13. Choi, S. E. and Lee J. M.: Standardization for the preparation of traditional Jeung-pyun. Korean J. Food Sci. Technol. 1993, 25, 655-665.
  14. Han, J. Y., Sung, J. H., Kim, D. J., Jeong, H. S., and Lee J. S.: Inhibitory effect of methanol extract and its fractions from grape seeds on mushroom tyrosinase. J. Korean Soc. Food Sci. Nutr. 2008, 37, 1679-1683. https://doi.org/10.3746/jkfn.2008.37.12.1679
  15. Choi, S. W., Chung, U. S., and Lee, K. T.: Preparation of high quality grape seed oil by solvent extraction and chemical refining process. Korean J. Food Preserv. 2005, 12, 600-607.
  16. Lee, E. J. and Kwon, J. H.: Characteristics of microwaveassisted extraction for grape seed components with different solvents. Korean J. Food Preserv. 2006, 13, 216-222.
  17. Jeong, S. M., Kim, S. Y., Ha, J. U., and Lee, S.C.: Effect of far-infrared irradiation on the antioxidant activity of extracts from grape seed. J. Korean Soc. Food Sci. Nutr. 2005, 34, 1619-1624. https://doi.org/10.3746/jkfn.2005.34.10.1619
  18. Cho, S. H., Kim, C. H., and Park, W. P.: Antimicrobial activities of botanical antimicrobial agent-grapefruit seed extract mixture for the preparation of anitimicrobial packaging paper. Korean J. Food Preserv. 2004, 411-416.
  19. Kim, K. B., Yoo, K. H., Park, H. Y., and Jeong, J. M.: Antioxidative activities of commercial edible plant extracts distributed in Korea. J. Korean Soc. Appl. Biol. Chem. 2006, 49, 328-333.
  20. Jang, Y. S. and Jeong, J. M.: Antioxidative effect and digestive enzyme inhibition of grape seed extract (GSE). J. Korean Soc. Food Sci. Nutr. 2010, 783-788.
  21. Lee, H. S., Lee, S. J., Sohn, J. H., Yu, H. J., and Cho, H. Y.: Effects of mixed Scutellaria baicalensis extracts as natural preservative on efficacy and storage of lactic acid-fermented garlic extract. J. Korean Soc. Food Sci. Nutr. 2017, 46, 10-17. https://doi.org/10.3746/jkfn.2017.46.1.010
  22. Choi, O. K., Noh, Y. C., and Hwang, S. Y.: Antimicrobial activity of grapefruit seed extracts and polylysine mixture against food-borne pathogens. Korean J. Diet. Culture 2000, 15, 9-15.
  23. Yeon, J. H., Lee, J. Y., Lee, H. S., Ha, S. D., Park, C. S., Woo, M. J., Lee, S. H., Kim, J. S., and Lee, C.: Evaluation of the natural antimicrobials against yeasts in functional beverages to control quality loss. J. Food Hyg. Safety 2009, 24, 273-276.
  24. Kim, K. M., Hwang, K. T., You, S. G., Lee, U. S., Jung, K. H., Moon, S. K., and Choi, W. S.: Antimicrobial effect of edible pullulan film containing natural antimicrobial material on cariogenic bacteria. J. Korean Soc. Food Sci. Nutr. 2009, 38, 1466-1470. https://doi.org/10.3746/jkfn.2009.38.10.1466
  25. Hwang, H. Y. and Jeon, D. W.: Effect of chitosan and mordant treatments on the air-permeability, fastness and antimicrobial effect of cotton and nylon fabrics dyed using Japanese pagoda tree. J. Fashion Business 2006, 10, 27-39.
  26. Kim, M. R.: Antimicrobial activity of PHB/chitosan films and quality of white bread packaged with the films. Korean J. Human Ecology 2005, 14, 321-330.
  27. Kim, J. S., Park, J. W., Park, S. H., Choi, D. S., Kim, Y. H., Lee, S. J., Park, C. W., Lee, J. S., and Cho, B. K.: Effects of modified atmosphere packaging (MAP) and vaporized ethyl pyruvate (EP) treatment for the shelf life of 'Seolhyang' strawberries. Korean J. Food Preserv. 2017, 4, 351-360.
  28. Sani, M. A., Saenger, C., Juretic, D., and Separovic, F.: Glycine substitution reduces antimicrobial activity and helical stretch of diPGLa-H in lipid micelles. J. Phys. Chem. B. 2017, 121, 4817-4822. https://doi.org/10.1021/acs.jpcb.7b03067
  29. Ilic, N., Novkovic, M., Guida, F., Xhindoli, D., Benincasa, M., Tossi, A., and Juretic, D.: Selective antimicrobial activity and mode of action of adepantins, glycine-rich peptide antibiotics based on anuran antimicrobial peptide sequences. Biochim Biophys Acta 2013, 1828, 1004-1012. https://doi.org/10.1016/j.bbamem.2012.11.017
  30. Babu, S. P., Sathiyamoorthy, S., Palanisamy, P., Boopathi, S., and Rajaram, V.: Antioxidant and antimicrobial properties of Glycine max-A review. Int. J. Cur. Bio. Med. Sci. 2011, 1, 49-62.
  31. Yoon, J. H., Jung, D. C., Lee, E. H., Kang, Y. S., Lee, S. Y., Park, S. R., Yeom, H. J., Ha, M. S., Park, S. K., Lee, Y. S., Ha, S. D., Kim, G. H., and Bae, D. H., Characteristics of a black soybean (Glycine max L. Merrill) protein isolate partially hydrolyzed by alcalase. Food Sci. Biotechnol. 2005, 12, 578-580.
  32. Sireerat, L. and Nirasha, H. P.: Antimicrobial activity of raw soybean, soybean flour and roasted soybean extracted by ethanol- hexane method. Br. Food J. 2017, 119, 2277-2286. https://doi.org/10.1108/BFJ-10-2016-0499

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