한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제51권4호
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  • Pages.397-403
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  • 2018
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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제주지역 양식 넙치(Paralichthys olivaceus)에서 분리한 어병세균 내 Erythromycin 내성 유전자 분석

Analysis of Erythromycin Resistance Gene in Pathogenic Bacteria Isolates from Cultured Olive flounder Paralichthys olivaceus in Jeju

  • 이다원 (제주대학교 해양의생명과학부) ;
  • 전려진 (제주대학교 해양의생명과학부) ;
  • 김승민 (주식회사 우진비앤지) ;
  • 정준범 (제주대학교 해양의생명과학부)
  • Lee, Da Won (School of Marine Biomedical Sciences, Jeju National University) ;
  • Jun, Lyu Jin (School of Marine Biomedical Sciences, Jeju National University) ;
  • Kim, Seung Min (Central Research Institute, Woo Gene B&G) ;
  • Jeong, Joon Bum (School of Marine Biomedical Sciences, Jeju National University)
  • 투고 : 2018.07.02
  • 심사 : 2018.08.14
  • 발행 : 2018.08.31
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초록

We determined the resistance rates of pathogenic bacteria isolated from cultured olive flounder Paralichthys olivaceus to erythromycin (Em), antibiotic typically used in aquaculture and analyzed the genotypes of resistant bacteria using polymerase chain reaction (PCR). We isolated and utilized 160 isolates of Streptococcus parauberis, 1 of S. iniae, 66 of Edwardsiella tarda, 56 of Vibrio sp. and 23 of unidentified bacteria from presumed infected olive flounder from Jeju Island from March 2016 to October 2017. Of the 306 isolated strains, Em-resistant strains included 33 of S. parauberis, 39 of E. tarda and 2 of Vibrio sp. We conducted PCR to assess the resistance determination of Em-resistant strains. Five different types of Em-resistance genes were detected in the 74 Em-resistant strains: erm (A), erm (B), erm (C), mef (A) and mef (E); erm (A) and erm (B) were detected in 1 (3%) and 24 (72.7%) S. parauberis isolates, respectively. In E. tarda, erm (B) was detected in five isolates (12.8 %) and no Em-resistance genes were detected in the two Vibrio sp. isolates.

키워드

참고문헌

  1. APQA (Animal and Plant Quarantine Agency). 2009. National antibiotic use and monitoring of antimicrobial resistance in 2015 [Internet]. Retrieved from www.qia.go.kr on Jun 18, 2018.
  2. Blanco MM, Gibello A, Vela AI, Moreno MA, Dominguez L and Fernandez-Garayzabal JF. 2002. PCR detection and PFGE DNA macrorestriction analyses of clinical isolates of Pseudomonas anguilliseptica from winter disease outbreaks in sea bream Sparus aurata. Dis Aquat Org 50, 19-27. https://dx.doi.org/10.3354/dao050019.
  3. Cepeda C, Garcia-Marquez S and Santos Y. 2003. Detection of Flexibacter maritimus in fish tissue using nested PCR amplification. J Fish Dis 26, 65-70. https://dx.doi.org/10.1046/j.1365-2761.2003.00431.x.
  4. CLSI (Clinical and Laboratory Standards Institute). 2017. Performance standards for antimicrobial susceptibility testing, 28th edition [Internet]. Retrieved from www.clsi.org on Jun 14, 2018.
  5. Demircan D and Candan A. 2006. Identification of Vibrio anguilliarum by PCR (rpoN Gene) associated with Vibriosis in marine fish in Turkey. Turk J Vet Anim Sci 30, 305-310.
  6. Domeenech A, Derenaaandez-Garayzabal JF, Pascual C, Garcia JA, Cutuli MT, Moreno MA, Collins MD and Dominguez L. 1996. Streptococcosis in cultured turbot, Scopthalmus maximus, associated with Streptococcus parauberis. J Fish Dis 19, 33-38. https://dx.doi.org/10.1111/j.1365-2761.1996.tb00117.x.
  7. Eldar A and Ghittino C. 1999. Lactococcus garvieae and Streptococcus iniae infections in rainbow trout Oncorhynchus mykiss: similar, but different diseases. Dis Aquat Org 36, 227-231. https://dx.doi.org/10.3354/dao36227.
  8. Han AR, Yoon YJ and Kim JW. 2012. Antibiotic Resistance and Plasmid Profile of Vibrio parahaemolyticus Strains Isolated from Kyunggi-Incheon Coastal Area. Kor J Microbiol 48, 22-28. https://dx.doi.org/10.7845/kjm.2012.48.1.022.
  9. Hansen LH, Mauvais P and Douthwaite S. 1999. The macrolide-ketolide antibiotic binding site is formed by structures in domains II and V of 23S ribosomal RNA. Mol Microbiol 31, 623-631. https://dx.doi.org/10.1046/j.1365-2958.1999.01202.x.
  10. Hiroshi N. 1996. Multidrug Efflux Pumps of Gram-Negative Bacteria. J Bacteriol 178, 5853-5859. https://doi.org/10.1128/jb.178.20.5853-5859.1996
  11. Jeong YU, Kang CY, Kim MJ, Heo MS, Oh DC and Kang BJ. 2006. Characterization of Streptococcosis occurrence and molecular identification of the pathogens of cultured flounder in Jeju island. J Microbiol 42, 199-204.
  12. Jo MR. 2006. Rapid molecular identification and antibiotic resistance of Vibrio spp. Isolated from the farmed olive flounders in Jeju island, Korea. Master's Thesis, Jeju National University, Jeju, Korea.
  13. Jun LJ. 2010. Characterization of antibiotic resistant genes carried by fish pathogens in Korea. Ph. D. Thesis, Pukyong National University, Busan, Korea.
  14. Kim HJ, Ryu JO, Lee SY, Kim ES and Kim HY. 2015. Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics. BMC Microbiol 15, 239. https://dx.doi.org/10.1186/s12866-015--577-3.
  15. Kim MS, Cho JY and Choi HS. 2014. Identification of Vibrio harveyi, Vibrio ichthyoenteri and Photobacterium damselae isolated from olive flounder Paralichthys olivaceus in Korea by multiplex PCR developed using the rpoB gene. J Fish Sci 80, 333-339. https://dx.doi.org/10.1007/s12562-014-0702-5.
  16. Lee SY and Kim YH. 2013. Incidence of Erythromycin Resistance Genes, erm(B) and mef(A), in Streptococci Isolated from Dental Plaques of Koreans. Int J Oral Biol 38, 61-65. https://dx.doi.org/10.11620/IJOB.2013.38.2.061.
  17. Lee DW, Jun LJ and Jeong JB. 2016. Distribution of Tetracycline Resistance Genes in Pathogenic Bacteria Isolated from Cultured Olive Flounder (Paralichthys olivaceus) in Jeju in 2016. JFMSE 29, 834-846. https://dx.doi.org/10.13000/JFMSE.2017.29.3.834.
  18. Lee HI, Jung JH, Lee SJ and Choi SS. 2010a. Analysis of Genotype and Phenotype of Erythromycin Resistance in Enterococci spp. Isolated from Raw Milk Samples. Kor J Microbiol 46, 148-151.
  19. Lee HY, Lim JE, Kim SC, Kim KR, Lee SS, Kwon OK, Yang JE and Ok YS. 2010b. Environmental Monitoring of Selected Veterinary Antibiotics in Soils, Sediments and Water Adjacent to a Poultry Manure Composting Facility in Gangwon Province, Korea. J Korean Soc Environ Eng 32, 278-286.
  20. Luna VA, Coates P, Eady EA, Cove JH, Nguyen TH and Roberts MC. 1999. A variety of Gram-positive bacteria carry mobile mef genes. J Antimicro Chemother 44, 19-25. https://dx.doi.org/10.1093/jac/44.1.19.
  21. Nonaka L, Maruyama F, Suzuki S and Masuda M. 2015. Novel macrolide-resistance genes, mef (C) and mph (G), carried by plasmids from Vibrio and Photobacterium isolated from sediment and seawater of a coastal aquaculture site. Appl Microbiol 61, 1-6. https://onlinelibrary.wiley.com/doi/abs/10.1111/lam.12414.
  22. Portillo A, Ruiz-Larrea F, Zarazaga M, Alonso A, Martinez JL and Torres C. 2000. Macrolide Resistance Genes in Enterococcus spp.. Antimicrob Agents Chemother 44, 967-971. https://dx.doi.org/10.1128/AAC.44.4.
  23. Sakai T, Iida T, Osatomi K and Kanai K. 2007. Detection of type 1 fimbrial genes in fish pathogenic and non-pathogenic Edwardsiella tarda strains by PCR. Fish Pathol 42, 115-117. https://dx.doi.org/ 10.3147/jsfp.42.115.
  24. Sapkota AR, Ojo KK, Roberts MC and Schwab KJ. 2006. Antibiotic resistance genes in multidrug-resistant Enterococcus spp. And Streptococcus spp. Recovered from the indoor air of a large-scale swine-feeding operation. Lett Appl Microbiol 43, 534-540. https://dx.doi.org/10.1111/j.1472-765X.2006.01996.x.
  25. Sung CH, Chon JW, Kwak HS, Kim HS and Seo KH. 2013. Prevalence and Antimicrobial Resistance of Enterococcus faecalis and Enterococcus faecium Isolated from Beef, Pork, Chicken and Sashimi. Korean J Food Sci 33, 133-138. https://dx.doi.org/ 10.5851/kosfa.2013.33.1.133.
  26. Thompson FL, Iida T and Swings J. 2004. Biodiversity of Vibrios. Microbiol Mol Biol Rev 68, 403-431. https://dx.doi.org/10.1128/MMBR.68.3.
  27. Woo SH, Kim HJ, Lee JS, Kim JW and Park SI. 2006. Pathogenicity and classification of streptococci isolated from cultured marine fishes. J Fish Pathol 19, 17-33.