한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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견과류로부터 효율적인 DNA 추출 방법 비교

Comparison of methods of DNA extraction from tree nuts

  • 서승만 (경희대학교 생명자원과학연구원 식품생명공학과) ;
  • 박샛별 (경희대학교 생명자원과학연구원 식품생명공학과) ;
  • 김미주 (경희대학교 생명자원과학연구원 식품생명공학과) ;
  • 김해영 (경희대학교 생명자원과학연구원 식품생명공학과)
  • Suh, Seung-Man (Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Park, Saet-Byul (Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Mi-Ju (Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University)
  • 투고 : 2018.02.25
  • 심사 : 2018.06.14
  • 발행 : 2018.08.31
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초록

본 연구에서는 견과류로부터 식품 분석에 사용될 DNA를 4가지 방법으로 추출하고 그 효율을 비교하였다. 동일한 양의 시료를 사용하여 추출된 DNA의 양은 CTAB법이 가장 우수한 것으로 확인되었지만, 추출 시간이 수배이상 오래 걸리고 유기용매를 사용한다는 한계점이 있다. 다른 방법들과 DNA 추출 양의 차이가 큰 잣, 캐슈너트, 피스타치오 너트, 땅콩의 시료는 CTAB법이 가장 효율적인 방법으로 판단되며, 호두, 헤이즐넛, 아몬드의 시료는 변형 CTAB법과 실리카 막법이 CTAB법을 대체할 수 있을 것으로 판단된다. 추출된 DNA를 식물 내재유전자 및 각 견과류에 특이적인 유전자를 사용하여 PCR을 진행하였으며, 모든 추출 방법에서 DNA가 정상적으로 증폭되는 것을 확인하였다.

This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.

키워드

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