Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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Biodegradable Screws Containing Bone Morphogenetic Protein-2 in an Osteoporotic Rat Model

  • Jin, Eun-Sun (Department of Internal Medicine, Kyung Hee University School of Medicine) ;
  • Kim, Ji Yeon (Laboratory of Stem Cell Therapy, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Lee, Bora (Department of Biostatistic Consulting, Soon Chun Hyang Medical Center) ;
  • Min, JoongKee (Laboratory of Stem Cell Therapy, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Jeon, Sang Ryong (Laboratory of Stem Cell Therapy, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Choi, Kyoung Hyo (Laboratory of Stem Cell Therapy, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Jeong, Je Hoon (Laboratory of Stem Cell Therapy, Asan Medical Center, University of Ulsan College of Medicine)
  • Received : 2017.11.30
  • Accepted : 2018.02.09
  • Published : 2018.09.01
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Abstract

Objective : The aim of this study was to evaluate the effect for biodegradable screws containing bone morphogenetic protein-2 (BMP-2) in an osteoporotic rat model. Methods : Twenty-four female Wistar rat (250-300 g, 12 weeks of age) were randomized into four groups. Three groups underwent bilateral ovariectomy (OVX). Biodegradable screws with or without BMP-2 were inserted in the proximal tibia in two implantation groups. The extracted proximal metaphysis of the tibiae were scanned by exo-vivo micro-computed tomography. Evaluated parameters included bone mineral density (BMD), trabecular bone volume (BV/TV), trabecular number, trabecular thickness, and trabecular separation (Tb.Sp). The tibia samples were pathologically evaluated by staining with by Hematoxylin and Eosin, and trichrome. Results : Trabecular formation near screw insertion site was evident only in rats receiving BMP-2 screws. BMD and BV/TV significantly differed between controls and the OVX and OVX with screw groups. However, there were no significant differences between control and OVX with screw BMP groups. Tb.Sp significantly differed between control and OVX and OVX with screw groups (p<0.05), and between the OVX and OVX with screw BMP group (p<0.05), with no statistically significant difference between control and OVX with screw BMP groups. Over the 12 weeks after surgery, bone lamellae in direct contact with the screw developed more extensive and thicker trabecular bone around the implant in the OVX with screw BMP group compared to the OVX with screw group. Conclusion : Biodegradable screws containing BMP-2 improve nearby bone conditions and enhance ostoeintegration between the implant and the osteoporotic bone.

Keywords

References

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