Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effects of Curcuma xanthorrhiza Supercritical Extract and Xanthorrhizol on LPS-Induced Inflammation in HGF-1 Cells and RANKL-Induced Osteoclastogenesis in RAW264.7 Cells

  • Kim, Siyeon (Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University) ;
  • Kook, Kyo Eun (Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University) ;
  • Kim, Changhee (Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University) ;
  • Hwang, Jae-Kwan (Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University)
  • Received : 2018.03.29
  • Accepted : 2018.05.25
  • Published : 2018.08.28
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Abstract

Periodontal disease is triggered by the host immune response to pathogens in the microbial biofilm. Worsening of periodontal disease destroys the tooth-supporting tissues and alveolar bone. As oral inflammation can induce systemic diseases in humans, it is important to prevent periodontal disease. In this study, we demonstrated that Curcuma xanthorrhiza supercritical extract (CXS) and its active compound, xanthorrhizol (XAN), exhibit anti-inflammatory effects on lipopolysaccharide (LPS)-treated human gingival fibroblast-1 cells and anti-osteoclastic effects on receptor activator of nuclear factor kappa B ligand (RANKL)-treated RAW264.7 cells. LPS-upregulated inflammatory factors, such as nuclear factor kappa B p65 and $interleukin-1{\beta}$, were prominently reduced by CXS and XAN. In addition, RANKL-induced osteoclastic factors, such as nuclear factor of activated T-cells c1, tartrate-resistant acid phosphatase, and cathepsin K, were decreased in the presence of CXS and XAN. CXS and XAN inhibited the mitogen-activated protein kinase (MAPK)/activator protein-1 (AP-1) signaling pathway. Collectively, these results provide evidence that CXS and XAN suppress LPS-induced inflammation and RANKL-induced osteoclastogenesis by suppressing the MAPK/AP-1 pathway.

Keywords

References

  1. Hasan A, Palmer R. 2014. A clinical guide to periodontology: pathology of periodontal disease. Br. Dent. J. 216: 457-461. https://doi.org/10.1038/sj.bdj.2014.299
  2. Paster BJ, Olsen I, Aas JA, Dewhirst FE. 2006. The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol. 2000 42: 80-87. https://doi.org/10.1111/j.1600-0757.2006.00174.x
  3. Kato H, Taguchi Y, Tominaga K, Umeda M, Tanaka A. 2014. Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Arch. Oral Biol. 59: 167-175. https://doi.org/10.1016/j.archoralbio.2013.11.008
  4. Li Q, Valerio MS, Kirkwood KL. 2012. MAPK usage in periodontal disease progression. J. Signal Transduct. 2012: 308943.
  5. Kim H, Kim MB, Kim C, Hwang JK. 2018. Inhibitory effects of panduratin A on periodontitis-induced inflammation and osteoclastogenesis through inhibition of MAPK pathways in vitro. J. Microbiol. Biotechnol. 28: 190-198. https://doi.org/10.4014/jmb.1707.07042
  6. He X, Andersson G, Lindgren U, Li Y. 2010. Resveratrol prevents RANKL-induced osteoclast differentiation of murine osteoclast progenitor RAW264.7 cells through inhibition of ROS production. Biochem. Biophys. Res. Commun. 401: 356-362. https://doi.org/10.1016/j.bbrc.2010.09.053
  7. Lee K, Chung YH, Ahn H, Kim H, Rho J, Jeong D. 2016. Selective regulation of MAPK signaling mediates RANKLdependent osteoclast differentiation. Int. J. Biol. Sci. 12: 235-245. https://doi.org/10.7150/ijbs.13814
  8. Boyce BF. 2013. Advances in osteoclast biology reveal potential new drug targets and new roles for osteoclasts. J. Bone Miner. Res. 28: 711-722. https://doi.org/10.1002/jbmr.1885
  9. Algate K, Haynes D, Bartold P, Crotti T, Cantley M. 2016. The effects of tumour necrosis factor-${\alpha}$ on bone cells involved in periodontal alveolar bone loss; osteoclasts, osteoblasts and osteocytes. J. Periodontal. Res. 51: 549-566. https://doi.org/10.1111/jre.12339
  10. Oon SF, Nallappan M, Tee TT, Shohaimi S, Kassim NK, Sa'ariwijaya MSF, et al. 2015. Xanthorrhizol: a review of its pharmacological activities and anticancer properties. Cancer Cell Int. 15: 100-114. https://doi.org/10.1186/s12935-015-0255-4
  11. Lim CS, Jin DQ, Mok H, Oh SJ, Lee JU, Hwang JK, et al. 2005. Antioxidant and antiinflammatory activities of xanthorrhizol in hippocampal neurons and primary cultured microglia. J. Neurosci. Res. 82: 831-838. https://doi.org/10.1002/jnr.20692
  12. Park JH, Jung YJ, Shrestha S, Lee SM, Lee TH, Lee CH, et al. 2014. Inhibition of NO production in LPS-stimulated RAW264.7 macrophage cells with curcuminoids and xanthorrhizol from the rhizome of Curcuma xanthorrhiza Roxb. and quantitative analysis using HPLC. J. Korean Soc. Appl. Biol. Chem. 57: 407-412. https://doi.org/10.1007/s13765-014-4132-y
  13. Choi MA, Kim SH, Chung WY, Hwang JK, Park KK. 2004. Xanthorrhizol, a natural sesquiterpenoid from Curcuma xanthorrhiza, has an anti-metastatic potential in experimental mouse lung metastasis model. Biochem. Biophys. Res. Commun. 326: 210-217. https://doi.org/10.1016/j.bbrc.2004.11.020
  14. Chung WY, Park JH, Kim MJ, Kim HO, Hwang JK, Lee SK, et al. 2007. Xanthorrhizol inhibits 12-O-tetradecanoylphorbol-13-acetate-induced acute inflammation and two-stage mouse skin carcinogenesis by blocking the expression of ornithine decarboxylase, cyclooxygenase-2 and inducible nitric oxide synthase through mitogen-activated protein kinases and/or the nuclear factor-${\kappa}$B. Carcinogenesis 28: 1224-1231. https://doi.org/10.1093/carcin/bgm005
  15. Hong KO, Hwang JK, Park KK, Kim SH. 2005. Phosphorylation of c-Jun N-terminal kinases (JNKs) is involved in the preventive effect of xanthorrhizol on cisplatin-induced hepatotoxicity. Arch. Toxicol. 79: 231-236. https://doi.org/10.1007/s00204-004-0623-7
  16. Mary HP, Susheela GK, Jayasree S, Nizzy A, Rajagopal B, Jeeva S. 2012. Phytochemical characterization and antimicrobial activity of Curcuma xanthorrhiza Roxb. Asian Pac. J. Trop. Biomed. 2: S637-S640. https://doi.org/10.1016/S2221-1691(12)60288-3
  17. Kida Y, Kobayashi M, Suzuki T, Takeshita A, Okamatsu Y, Hanazawa S, et al. 2005. Interleukin-1 stimulates cytokines, prostaglandin E2 and matrix metalloproteinase-1 production via activation of MAPK/AP-1 and NF-kappaB in human gingival fibroblasts. Cytokine 29: 159-168. https://doi.org/10.1016/j.cyto.2004.10.009
  18. Hajishengallis G. 2015. Periodontitis: from microbial immune subversion to systemic inflammation. Nat. Rev. Immunol. 15: 30-44. https://doi.org/10.1038/nri3785
  19. Cetinkaya B, Guzeldemir E, Ogus E, Bulut S. 2013. Proinflammatory and anti-inflammatory cytokines in gingival crevicular fluid and serum of patients with rheumatoid arthritis and patients with chronic periodontitis. J. Periodontol. 84: 84-93. https://doi.org/10.1902/jop.2012.110467
  20. Dietrich T, Jimenez M, Kaye EAK, Vokonas PS, Garcia RI. 2008. Age-dependent associations between chronic periodontitis/edentulism and risk of coronary heart disease. Circulation 117: 1668-1674. https://doi.org/10.1161/CIRCULATIONAHA.107.711507
  21. Southerland JH, Taylor GW, Moss K, Beck JD, Offenbacher S. 2006. Commonality in chronic inflammatory diseases: periodontitis, diabetes, and coronary artery disease. Periodontol. 2000 40: 130-143. https://doi.org/10.1111/j.1600-0757.2005.00138.x
  22. Watts A, Crimmins EM, Gatz M. 2008. Inflammation as a potential mediator for the association between periodontal disease and Alzheimer's disease. Neuropsychiatr. Dis. Treat. 4: 865-876.
  23. Jantan I, Saputri FC, Qaisar MN, Buang F. 2012. Correlation between chemical composition of Curcuma domestica and Curcuma xanthorrhiza and their antioxidant effect on human low-density lipoprotein oxidation. Evid. Based Complement. Alternat. Med. 2012: 438356
  24. Rufino AT, Ribeiro M, Judas F, Salgueiro L, Lopes MC, Cavaleiro C, et al. 2014. Anti-inflammatory and chondroprotective activity of (+)-${\kappa}$-pinene: structural and enantiomeric selectivity. J. Nat. Prod. 77: 264-269. https://doi.org/10.1021/np400828x
  25. Ehrnhofer-Ressler MM, Fricke K, Pignitter M, Walker JM, Walker J, Rychlik M, et al. 2013. Identification of 1,8-cineole, borneol, camphor, and thujone as anti-inflammatory compounds in a Salvia officinalis L. infusion using human gingival fibroblasts. J. Agric. Food. Chem. 61: 3451-3459. https://doi.org/10.1021/jf305472t
  26. Chun KS, Keum YS, Han SS, Song YS, Kim SH, Surh YJ. 2003. Curcumin inhibits phorbol ester-induced expression of cyclooxygenase-1 in mouse skin through suppression of extracellular signal-regulated kinase activity and NF-${\kappa}$B activation. Carcinogenesis 24:1515-1524. https://doi.org/10.1093/carcin/bgg107
  27. Nakajima Y, Furuichi Y, Biswas KK, Hashiguchi T, Kawahara K-I, Yamaji K, et al. 2006. Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-${\kappa}$B pathway inhibition. FEBS Lett. 580: 613-619. https://doi.org/10.1016/j.febslet.2005.12.079
  28. Baker RG, Hayden MS, Ghosh S. 2011. NF-${\kappa}$B, inflammation, and metabolic disease. Cell Metab. 13: 11-22. https://doi.org/10.1016/j.cmet.2010.12.008
  29. Baron R, Kneissel M. 2013. WNT signaling in bone homeostasis and disease: from human mutations to treatments. Nat. Med. 19: 179-192. https://doi.org/10.1038/nm.3074

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