Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Expression and regulation of avian beta-defensin 8 protein in immune tissues and cell lines of chickens

  • Rengaraj, Deivendran (Department of Animal Science and Technology, Chung-Ang University) ;
  • Truong, Anh Duc (Department of Animal Science and Technology, Chung-Ang University) ;
  • Lillehoj, Hyun S. (Animal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture) ;
  • Han, Jae Yong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Hong, Yeong Ho (Department of Animal Science and Technology, Chung-Ang University)
  • Received : 2017.11.15
  • Accepted : 2018.03.05
  • Published : 2018.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: Defensins are a large family of antimicrobial peptides and components of the innate immune system that invoke an immediate immune response against harmful pathogens. Defensins are classified into alpha-, beta-, and theta-defensins. Avian species only possess beta-defensins (AvBDs), and approximately 14 AvBDs (AvBD1-AvBD14) have been identified in chickens to date. Although substantial information is available on the conservation and phylogenetics, limited information is available on the expression and regulation of AvBD8 in chicken immune tissues and cells. Methods: We examined AvBD8 protein expression in immune tissues of White Leghorn chickens (WL) by immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (RT-qPCR). In addition, we examined AvBD8 expression in chicken T-, B-, macrophage-, and fibroblast-cell lines and its regulation in these cells after lipopolysaccharide (LPS) treatment by immunocytochemistry and RT-qPCR. Results: Our results showed that chicken AvBD8 protein was strongly expressed in the WL intestine and in macrophages. AvBD8 gene expression was highly upregulated in macrophages treated with different LPS concentrations compared with that in T- and B-cell lines in a time-independent manner. Moreover, chicken AvBD8 strongly interacted with other AvBDs and with other antimicrobial peptides as determined by bioinformatics. Conclusion: Our study provides the expression and regulation of chicken AvBD8 protein in immune tissues and cells, which play crucial role in the innate immunity.

Keywords

References

  1. Su S, Dwyer DM, Miska KB, et al. Expression of host defense peptides in the intestine of Eimeria-challenged chickens. Poult Sci 2017;96:2421-7. https://doi.org/10.3382/ps/pew468
  2. Lee MO, Jang HJ, Rengaraj D, et al. Tissue expression and antibacterial activity of host defense peptides in chicken. BMC Vet Res 2016;12:231. https://doi.org/10.1186/s12917-016-0866-6
  3. Harwig SS, Swiderek KM, Kokryakov VN, et al. Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes. FEBS Lett 1994;342:281-5. https://doi.org/10.1016/0014-5793(94)80517-2
  4. Lynn DJ, Higgs R, Gaines S, et al. Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken. Immunogenetics 2004;56:170-7. https://doi.org/10.1007/s00251-004-0675-0
  5. Xiao Y, Hughes AL, Ando J, et al. A genome-wide screen identifies a single beta-defensin gene cluster in the chicken: implications for the origin and evolution of mammalian defensins. BMC Genomics 2004;5:56. https://doi.org/10.1186/1471-2164-5-56
  6. Hong YH, Song W, Lee SH, Lillehoj HS. Differential gene expression profiles of beta-defensins in the crop, intestine, and spleen using a necrotic enteritis model in 2 commercial broiler chicken lines. Poult Sci 2012;91:1081-8. https://doi.org/10.3382/ps.2011-01948
  7. Lynn DJ, Higgs R, Lloyd AT, et al. Avian beta-defensin nomenclature: a community proposed update. Immunol Lett 2007;110:86-9. https://doi.org/10.1016/j.imlet.2007.03.007
  8. Higgs R, Lynn DJ, Gaines S, et al. The synthetic form of a novel chicken beta-defensin identified in silico is predominantly active against intestinal pathogens. Immunogenetics 2005;57:90-8. https://doi.org/10.1007/s00251-005-0777-3
  9. Cuperus T, Coorens M, van Dijk A, Haagsman HP. Avian host defense peptides. Dev Comp Immunol 2013;41:352-69. https://doi.org/10.1016/j.dci.2013.04.019
  10. Truong AD, Hong Y, Hoang CT, Lee J, Hong YH. Chicken IL-26 regulates immune responses through the JAK/STAT and NF-kappa B signaling pathways. Dev Comp Immunol 2017;73:10-20. https://doi.org/10.1016/j.dci.2017.03.001
  11. Lee HC, Kim SK, Park TS, et al. Compensatory proliferation of endogenous chicken primordial germ cells after elimination by busulfan treatment. Stem Cell Res Ther 2013;4:136. https://doi.org/10.1186/scrt347
  12. Szklarczyk D, Franceschini A, Wyder S, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res 2015;43:D447-52. https://doi.org/10.1093/nar/gku1003
  13. Rengaraj D, Lee BR, Han JY, Pang MG. Comprehensive analysis on the homology, interaction, and miRNA regulators of human deleted in azoospermia proteins: updated evolutionary relationships with primates. Genes Genomics 2017;39:1335-51. https://doi.org/10.1007/s13258-017-0598-4
  14. Derache C, Esnault E, Bonsergent C, et al. Differential modulation of beta-defensin gene expression by Salmonella Enteritidis in intestinal epithelial cells from resistant and susceptible chicken inbred lines. Dev Comp Immunol 2009;33:959-66. https://doi.org/10.1016/j.dci.2009.03.005
  15. Hamad SK, Kim S, El-Kadi SW, Wong EA, Dalloul RA. Comparative expression of host defense peptides in turkey poults. Poult Sci 2017;96:2083-90. https://doi.org/10.3382/ps/pew500
  16. Xu SZ, Lee SH, Lillehoj HS, Hong YH, Bravo D. Effects of dietary selenium on host response to necrotic enteritis in young broilers. Res Vet Sci 2015;98:66-73. https://doi.org/10.1016/j.rvsc.2014.12.004
  17. Aderem A, Ulevitch RJ. Toll-like receptors in the induction of the innate immune response. Nature 2000;406:782-7. https://doi.org/10.1038/35021228
  18. Rabsch W, Andrews HL, Kingsley RA, et al. Salmonella enterica serotype Typhimurium and its host-adapted variants. Infect Immun 2002;70:2249-55. https://doi.org/10.1128/IAI.70.5.2249-2255.2002
  19. Ronholm J, Zhang ZY, Cao XD, Lin M. Monoclonal antibodies to lipopolysaccharide antigens of Salmonella enterica serotype Typhimurium DT104. Hybridoma 2011;30:43-52. https://doi.org/10.1089/hyb.2010.0066
  20. Lu L, Li SM, Zhang L, et al. Expression of beta-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide. Genet Mol Res 2015;14:3330-7. https://doi.org/10.4238/2015.April.13.12
  21. Meade KG, Narciandi F, Cahalane S, et al. Comparative in vivo infection models yield insights on early host immune response to Campylobacter in chickens. Immunogenetics 2009;61:101-10. https://doi.org/10.1007/s00251-008-0346-7
  22. Higgs R, Lynn DJ, Cahalane S, et al. Modification of chicken avian beta-defensin-8 at positively selected amino acid sites enhances specific antimicrobial activity. Immunogenetics 2007; 59:573-80. https://doi.org/10.1007/s00251-007-0219-5
  23. van Dijk A, Veldhuizen EJ, Haagsman HP. Avian defensins. Vet Immunol Immunopathol 2008;124:1-18. https://doi.org/10.1016/j.vetimm.2007.12.006
  24. Derache C, Labas V, Aucagne V, et al. Primary structure and antibacterial activity of chicken bone marrow-derived betadefensins. Antimicrob Agents Chemother 2009;53:4647-55. https://doi.org/10.1128/AAC.00301-09
  25. Rengaraj D, Truong AD, Ban J, Lillehoj HS, Hong YH. Distribution and differential expression of microRNAs in the intestinal mucosal layer of necrotic enteritis induced Fayoumi chickens. Asian-Australas J Anim Sci 2017;30:1037-47. https://doi.org/10.5713/ajas.16.0685
  26. Sekelova Z, Stepanova H, Polansky O, et al. Differential protein expression in chicken macrophages and heterophils in vivo following infection with Salmonella Enteritidis. Vet Res 2017; 48:35. https://doi.org/10.1186/s13567-017-0439-0

Cited by

  1. Unusual interplay of contrasting selective pressures on β-defensin genes implicated in male fertility of the Buffalo (Bubalus bubalis) vol.19, pp.None, 2019, https://doi.org/10.1186/s12862-019-1535-8