Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Expression of selenium-independent glutathione peroxidase 5 (GPx5) in the epididymis of Small Tail Han sheep

  • Li, Ruilan (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Fan, Xiaomei (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Zhang, Tong (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Song, Huizi (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Bian, Xiaona (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Nai, Rile (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Li, Jinquan (College of Animal Science, Inner Mongolia Agricultural University) ;
  • Zhang, Jiaxin (College of Animal Science, Inner Mongolia Agricultural University)
  • Received : 2018.01.03
  • Accepted : 2018.03.29
  • Published : 2018.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: Selenium-independent glutathione peroxidase (GPx5) is specifically expressed in the mammalian epididymis and plays an important role in protecting sperm from reactive oxygen species and lipid peroxidation damage. This study investigates GPx5 expression in the epididymis of Small Tail Han sheep. Methods: GPx5 expression was studied in three age groups: lamb (2 to 3 months), young (8 to 10 months), and adult (18 to 24 months). The epididymis of each age group divided into caput, corpus and cauda, respectively. Analysis the expression quantity of GPx5 in epididymis and testis by real-time fluorescent quantitative polymerase chain reaction and Western blot. Finally, GPx5 protein locating in the epididymis by immunohistochemical. Results: The results demonstrate that in the lamb group, the GPx5 mRNA, but not protein, can be detected. GPx5 mRNA and expressed protein were detected in both the young and adult groups. Moreover, both the mRNA and protein levels of GPx5 were significantly higher in the young group than in other two groups. When the different segments of epididymis were investigated, GPx5 mRNA was expressed in each segment of epididymis regardless of age. Additionally, the mRNA level in the caput was significantly higher than that in corpus and cauda within same age group. The GPx5 protein was in the epithelial cells' cytoplasm. However, GPx5 mRNA and protein were not detected in the testis. Conclusion: These results suggest that GPx5 is mainly expressed in the epididymis of Small Tail Han sheep, and that the expression level of GPx5 is associated with age. Additionally, GPx5 was primarily expressed in the epithelial cells of the caput. Taken together, these studies indicate that GPx5 is expressed in the epididymis in all age grades.

Keywords

References

  1. Dacheux JL, Dacheux F. New insights into epididymal function in relation to sperm maturation. Reproduction 2013;147:27-42.
  2. Drevet JR. The antioxidant glutathione peroxidase family and spermatozoa: a complex story. Mol Cell Endocrinol 2006;250:70-9. https://doi.org/10.1016/j.mce.2005.12.027
  3. Fujii J, Tsunoda S. Redox regulation of fertilisation and the spermatogenic process. Asian J Androl 2011;13:420-3. https://doi.org/10.1038/aja.2011.10
  4. Ray PD, Huang BW, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal 2012;24:981-90. https://doi.org/10.1016/j.cellsig.2012.01.008
  5. Wright C, Milne S, Leeson H. Sperm DNA damage caused by oxidative stress: modifiable clinical, lifestyle and nutritional factors in male infertility. Reprod Biomed Online 2014;28:684-703. https://doi.org/10.1016/j.rbmo.2014.02.004
  6. Aitken RJ. Gpx5 protects the family jewels. J Clin Invest 2009;119:1849-51.
  7. Chabory E, Damon C, Lenoir A, et al. Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity. J Anim Sci 2010;88:1321-31. https://doi.org/10.2527/jas.2009-2583
  8. Molina LCP, Frechou DMS, Verstraeten SV. Early response of glutathione- and thioredoxin-dependent antioxidant defense systems to tl(i)- and tl(iii)-mediated oxidative stress in adherent pheochromocytoma (pc12adh) cells. Arch Toxicol 2018;92:195-211. https://doi.org/10.1007/s00204-017-2056-0
  9. Rejraji H, Vernet P, Drevet JR. GPX5 is present in the mouse caput and cauda epididymidis lumen at three different locations. Mol Reprod Dev 2002;63:96-103. https://doi.org/10.1002/mrd.10136
  10. Williams K, Frayne J, Hall L. Expression of extracellular glutathione peroxidase type 5 (GPX5) in the rat male reproductive tract. Mol Hum Reprod 1998;4:841-8. https://doi.org/10.1093/molehr/4.9.841
  11. Beiglbock A, Pera I, Ellerbrock K, Kirchhoff C. Dog epididymisspecific mrna encoding secretory glutathione peroxidaselike protein. J Reprod Fertil 1998;112:357-67. https://doi.org/10.1530/jrf.0.1120357
  12. Okamura N, Iwaki Y, Hiramoto S, et al. Molecular cloning and characterization of the epididymis-specific glutathione peroxidase-like protein secreted in the porcine epididymal fluid. Biochim Biophys Acta 1997;1336:99-109. https://doi.org/10.1016/S0304-4165(97)00016-0
  13. Chabory E, Damon C, Lenoir A, et al. Epididymis selenoindependent glutathione peroxidase 5 maintains sperm DNA integrity in mice. J Clin Invest 2009;119:2074-85.
  14. Brigelius-Flohe R, Maiorino M. Glutathione peroxidase. Biochim Biophys Acta 2013;1830: 3289-303. https://doi.org/10.1016/j.bbagen.2012.11.020
  15. Taylor A, Robson A, Houghton BC, et al. Epididymal specific, selenium-independent GPX5 protects cells from oxidative stress-induced lipid peroxidation and DNA mutation. Hum Reprod 2013;28:2332-42. https://doi.org/10.1093/humrep/det237
  16. Aitken RJ, Iuliis GND, Mclachlan RI. Biological and clinical significance of DNA damage in the male germ line. Int J Androl 2009;32:46-56. https://doi.org/10.1111/j.1365-2605.2008.00943.x
  17. Evenson D, Wixon R. Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay. Reprod Biomed Online 2006;12:466-72. https://doi.org/10.1016/S1472-6483(10)62000-7
  18. Fernandez-Gonzalez R, Moreira PN, Perez-Crespo M, et al. Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring. Biol Reprod 2008;78:761-72. https://doi.org/10.1095/biolreprod.107.065623
  19. Aitken RJ, Iuliis GND. On the possible origins of DNA damage in human spermatozoa. Mol Hum Reprod 2010;16:3-13. https://doi.org/10.1093/molehr/gap059
  20. Zini A, Libman J. Sperm DNA damage: clinical significance in the era of assisted reproduction. CMAJ 2006;175:495-500. https://doi.org/10.1503/cmaj.060218
  21. Seligman J, Newton GL, Fahey RC, Shalgi R, Kosower NS. Nonprotein thiols and disulfides in rat epididymal spermatozoa and epididymal fluid: role of ${\gamma}$-glutamyl-transpeptidase in sperm maturation. J Androl 2005;26:629-37. https://doi.org/10.2164/jandrol.05040
  22. Anais N, Noblanc A, Peltier M, et al. Epididymis response partly compensates for spermatozoa oxidative defects in snGPX4 and GPX5 double mutant mice. Plos One 2012;7:e38565. https://doi.org/10.1371/journal.pone.0038565
  23. Ghyselinck NB, Dufaure I, Lareyre JJ, et al. Structural organization and regulation of the gene for the androgen-dependent glutathione peroxidase-like protein specific to the mouse epididymis. Mol Endocrinol 1993;7:258-72.
  24. Belleannee, C, Labas, V, Teixeira-Gomes, AP, Gatti, JL, Dacheux, JL, Dacheux, F. Identification of luminal and secreted proteins in bull epididymis. J Proteomics 2011; 74, 59-78. https://doi.org/10.1016/j.jprot.2010.07.013
  25. Koziorowska-Gilun M, Fraser L, Gilun P, Koziorowski M, Kordan W. Activity of antioxidant enzymes and their mRNA expression in different reproductive tract tissues of the male roe deer (Capreolus capreolus) during the pre-rut and rut seasons. Small Rumin Res 2015;129:97-103. https://doi.org/10.1016/j.smallrumres.2015.05.006
  26. Koziorowska-Gilun M, Gilun P, Fraser L, et al. Antioxidant enzyme activity and mrna expression in reproductive tract of adult male european bison (Bison bonasus, linnaeus 1758). Reprod Domest Anim 2013;48:7-14. https://doi.org/10.1111/j.1439-0531.2012.02015.x
  27. Lefrancois AM, Jimenez C, Dufaure JP. Developmental expression and androgen regulation of 24 kda secretory proteins by the murine epididymis. Int J Androl 1993;16:147-54. https://doi.org/10.1111/j.1365-2605.1993.tb01168.x
  28. Bilaspuri GS. Histomorphology of the epididymis in prepubertal goats. Small Rumin Res 1993;10: 227-35. https://doi.org/10.1016/0921-4488(93)90127-4
  29. Vernet P, Faure J, Dufaure J, Drevet JR. Tissue and developmental distribution, dependence upon testicular factors and attachment to spermatozoa of GPX5, a murine epididymisspecific glutathione peroxidase. Mol Reprod Dev 1998;47:87-98.
  30. Weir CP, Robaire B. Spermatozoa have decreased antioxidant enzymatic capacity and increased reactive oxygen species production during aging in the brown norway rat. J Androl 2007;28:229-40.

Cited by

  1. Testosterone promotes GPX5 expression of goat epididymal epithelial cells cultured in vitro vol.55, pp.9, 2019, https://doi.org/10.1007/s11626-019-00391-y
  2. Trehalose can effectively protect sheep epididymis epithelial cells from oxidative stress vol.64, pp.2, 2018, https://doi.org/10.5194/aab-64-335-2021
  3. Oxidative Stress, Mitochondrial Function and Adaptation to Exercise: New Perspectives in Nutrition vol.11, pp.11, 2018, https://doi.org/10.3390/life11111269