한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제33권2호
  • /
  • Pages.61-68
  • /
  • 2018
  • /
  • 2508-755X(pISSN)
  • /
  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지
DOI QR코드

DOI QR Code

Detection of Matrix Metalloproteinases Patterns in Bovine Luteum cell during Pregnancy

  • Kim, Sang-Hwan (Institute of Genetic Engineering, Hankyong National University) ;
  • Kim, Kyong-Lae (Institute of Genetic Engineering, Hankyong National University) ;
  • Lee, Ji-Hye (Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Shin, Da-Hye (Department of Animal Life and Environment science, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Jung, Na-Hyeon (Department of Animal Life and Environment science, Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Lee, Ho-Jun (Institute of Genetic Engineering, Hankyong National University) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 투고 : 2018.06.09
  • 심사 : 2018.06.21
  • 발행 : 2018.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The major focus of this study is to analyze the expression of bovine MMPs and to monitor their activity during the estrus cycle and pregnancy. During pregnancy, MMP-2 expression was detectable around 30 days but became insignificant by 60 days, then started to increase again around 90 days and reached the maximum at 250 days. The activity of MMP-2 protein changed in accordance with its expression level. As expected, the level of TIMP-2 exhibited a reverse pattern. About MMP-9, high level expression was observed as early as 30 days and gradually increase until 90 days. Then started to decrease after 250 days. Again, the sites of MMP-9 expression were similar to those of MMP-2. On the other hand, expression of TIMP-3 remained low until 90 days but showed a small and temporal increase around 250 days. In summary, expression of different MMPs were differentially regulated during estrus cycle and pregnancy. While the expression of MMP-2 was high in estrus cycle, MMP-9 slowly takes over with the progression of pregnancy. These results indicated that the luteal tissue perform distinct functions during pregnancy and estrus. Perhaps the activity of MMP-2 is required for the structural remodeling of luteum, resulting the suppression of P4 inflow from blood. On the other hand, steady maintenance of MMP-9 throughout luteal development is important for the activation of cell proliferation, maturation and angiogenesis.

키워드

참고문헌

  1. Balasingam V, Yong VW. 1996. Attenuation of astroglial reactivity by interleukin-10. J Neurosci 16:2945-2955. https://doi.org/10.1523/JNEUROSCI.16-09-02945.1996
  2. Berisha B, Schams D. 2005. Ovarian function in ruminants. Domest Anim Endocrinol. 29(2):305-317. https://doi.org/10.1016/j.domaniend.2005.02.035
  3. Brenner CA, Adler RR, Rappolee DA, Pedersen RA, Werb Z. 1989. Gene for estracellular matrix degrading metalloproteinases and their inhibitor, TIMP, are expressed during earlymammalian development. Genes Dev. 3:845-859.
  4. Curry TE Jr, Osteen KG. 2003. The matrix metalloproteinase system: changes, regulation, and impact throughout the ovarian and uterine reproductive cycle. Endocr Rev. 24(4):428-465. https://doi.org/10.1210/er.2002-0005
  5. Huppertz B, Kertschansks S, Demir AY, Frank H-G, Kaufman P. 1998. Immunohistochemistry of matrix metallopro teinases (MMP), their substrates, and their inhibitors (TIMP) during trophoblast invasion in the human placenta. Cell Tissue Res. 291:133-148.
  6. Kim DS, Kim SH, Yoon JT. 2011. Expression of PAPP-A and 20a-HSD in the Bovine Corpus Luteurn during Early Pregnancy. J. Emb. Trans. 26(1): 57- 63.
  7. Kliem H , Welter H , Kraetzl W D , Steffl M , Meyer H H D, Schams D, Berisha B. 2007. Expression and localisation of extracellular matrix degrading proteases and their inhibitors during the oestrous cycle and after induced luteolysis in the bovine corpus luteum. Reproduction. 134:535-547. https://doi.org/10.1530/REP-06-0172
  8. Lei ZM, Chegini N, Ch V, Rac. 1991. Quantitative cell composition of human and bovine corpora lutea from various reproductive states. Biology of Reproduction. 44:1148-1156. https://doi.org/10.1095/biolreprod44.6.1148
  9. Matsushita H, Morishita R, Nata T, Aoki M, Nakagami H, Taniyama Y, Yamamoto K, Higaki J, Yasufumi K, Ogihara T. 2000. Hypoxia-induced endothelial apoptosis through nuclear factor-${\kappa}B$(NF-${\kappa}B$)-mediated bcl-2 suppression: in vivo evidence of the importance of NF-kappaB in endothelial cell regulation. Circ Res. 86:974-981. https://doi.org/10.1161/01.RES.86.9.974
  10. McCracken JA, Custer EE, Lamsa JC. 1999. Luteolysis: a neuroendocrine-mediated event. Physiol Rev. 79:263-323. https://doi.org/10.1152/physrev.1999.79.2.263
  11. Michaluk P, Kaczmarek L. 2007. Matrix metalloproteinase-9 in glutamate-dependent adult brain function and dysfunction. Cell Death Differ. 14(7):1255-1258. https://doi.org/10.1038/sj.cdd.4402141
  12. Nakajin S, Kawai Y, Ohno S, and Shinoda M. 1989. Purification and characterization og pig adrenal $20{\alpha}$-hydroxysteroid dehydrogenase. Journal of Steroid Biochemistry. 33:1181-1189. https://doi.org/10.1016/0022-4731(89)90428-7
  13. Pustovrh C, Jawerbaum A, Sinner D, White V, Capobianco E, Gonzalez E. 2002. Metalloproteinase 2 activity and modulation in uterus from neonatal streptozotocin-induced diabetic rats during embryo implantation. Reprod Fertil Dev. 14(7-8):479-485. https://doi.org/10.1071/RD02001
  14. Reynolds LP, Redmer DA. 1999. Growth and development of the corpus luteum. J, Reprod Fertil Suppl. 54:181-191.
  15. Richard L, Stouffer. 2006. Structure, Function, and Regulation of the Corpus Luteum. Knobil and Neill's Physiology of Reproduction (Third Edition). pp 475-526.
  16. Smith CC, Betteridge DJ, Nourooz-Zadeh J. 1999. The generation of the F(2)-isoprostane 8-epi-PGF(2alpha) by human platelets on collagen stimulation. Platelets. 10(4):253-256. https://doi.org/10.1080/09537109976112