Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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The relationship between low survival and acute increase of tumor necrosis factor α expression in the lung in a rat model of asphyxial cardiac arrest

  • Park, Yoonsoo (Department of Emergency Medicine and Institute of Medical Sciences, Kangwon National University Hospital, Kangwon National University School of Medicine) ;
  • Tae, Hyun-Jin (Bio Safety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Cho, Jeong Hwi (Bio Safety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, In-Shik (Bio Safety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Ohk, Taek Geun (Department of Emergency Medicine and Institute of Medical Sciences, Kangwon National University Hospital, Kangwon National University School of Medicine) ;
  • Park, Chan Woo (Department of Emergency Medicine and Institute of Medical Sciences, Kangwon National University Hospital, Kangwon National University School of Medicine) ;
  • Moon, Joong Bum (Department of Emergency Medicine and Institute of Medical Sciences, Kangwon National University Hospital, Kangwon National University School of Medicine) ;
  • Shin, Myoung Cheol (Department of Emergency Medicine and Institute of Medical Sciences, Kangwon National University Hospital, Kangwon National University School of Medicine) ;
  • Lee, Tae-Kyeong (Department of Neurobiology, Kangwon National University School of Medicine) ;
  • Lee, Jae-Chul (Department of Neurobiology, Kangwon National University School of Medicine) ;
  • Park, Joon Ha (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Ahn, Ji Hyeon (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kang, Seok Hoon (Department of Medical Education, Kangwon National University School of Medicine) ;
  • Won, Moo-Ho (Department of Neurobiology, Kangwon National University School of Medicine) ;
  • Cho, Jun Hwi (Department of Emergency Medicine and Institute of Medical Sciences, Kangwon National University Hospital, Kangwon National University School of Medicine)
  • Received : 2018.03.26
  • Accepted : 2018.05.12
  • Published : 2018.06.30
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Abstract

Cardiac arrest (CA) is sudden loss of heart function and abrupt stop in effective blood flow to the body. The patients who initially achieve return of spontaneous circulation (RoSC) after CA have low survival rate. It has been known that multiorgan dysfunctions after RoSC are associated with high morbidity and mortality. Most previous studies have focused on the heart and brain in RoSC after CA. Therefore, the aim of this research was to perform serological, physiological, and histopathology study in the lung and to determine whether or how pulmonary dysfunction is associated with low survival rate after CA. Experimental animals were divided into sham-operated group (n=14 at each point in time), which was not subjected to CA operation, and CA-operated group (n=14 at each point in time), which was subjected to CA. The rats in each group were sacrificed at 6 hours, 12 hours, 24 hours, and 2 days, respectively, after RoSC. Then, pathological changes of the lungs were analyzed by hematoxylin and eosin staining, Western blot and immunohistochemistry for tumor necrosis factor ${\alpha}$ (TNF-${\alpha}$). The survival rate after CA was decreased with time past. We found that histopathological score and TNF-${\alpha}$ immunoreactivity were significantly increased in the lung after CA. These results indicate that inflammation triggered by ischemia-reperfusion damage after CA leads to pulmonary injury/dysfunctions and contributes to low survival rate. In addition, the finding of increase in TNF-${\alpha}$ via inflammation in the lung after CA would be able to utilize therapeutic or diagnostic measures in the future.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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