Clinical and Experimental Reproductive Medicine

  • 제45권2호
  • /
  • Pages.75-81
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  • 2018
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Differentiation of human male germ cells from Wharton's jelly-derived mesenchymal stem cells

  • Dissanayake, DMAB (Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Kelaniya) ;
  • Patel, H (StemCure Pvt. Ltd.) ;
  • Wijesinghe, PS (Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Kelaniya)
  • 투고 : 2017.10.01
  • 심사 : 2018.05.03
  • 발행 : 2018.06.30
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초록

Objective: Recapitulation of the spermatogenesis process in vitro is a tool for studying the biology of germ cells, and may lead to promising therapeutic strategies in the future. In this study, we attempted to transdifferentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into male germ cells using all-trans retinoic acid and Sertoli cell-conditioned medium. Methods: Human WJ-MSCs were propagated by the explant culture method, and cells at the second passage were induced with differentiation medium containing all-trans retinoic acid for 2 weeks. Putative germ cells were cultured with Sertoli cell-conditioned medium at $36^{\circ}C$ for 3 more weeks. Results: The gene expression profile was consistent with the stage-specific development of germ cells. The expression of Oct4 and Plzf (early germ cell markers) was diminished, while Stra8 (a premeiotic marker), Scp3 (a meiotic marker), and Acr and Prm1 (postmeiotic markers) were upregulated during the induction period. In morphological studies, approximately 5% of the cells were secondary spermatocytes that had completed two stages of acrosome formation (the Golgi phase and the cap phase). A few spermatid-like cells that had undergone the initial stage of tail formation were also noted. Conclusion: Human WJ-MSCs can be transdifferentiated into more advanced stages of germ cells by a simple two-step induction protocol using retinoic acid and Sertoli cell-conditioned medium.

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참고문헌

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피인용 문헌

  1. Efficient generation of male germ-like cells derived during co-culturing of adipose-derived mesenchymal stem cells with Sertoli cells under retinoic acid and testosterone induction vol.10, pp.1, 2018, https://doi.org/10.1186/s13287-019-1181-5
  2. Potential use of stem cells for fertility preservation vol.8, pp.4, 2018, https://doi.org/10.1111/andr.12713
  3. Differentiation potential of adipose tissue‐derived mesenchymal stem cells into germ cells with and without growth factors vol.53, pp.1, 2018, https://doi.org/10.1111/and.13892
  4. Mesenchymal stromal/stem cells and their exosomes for restoration of spermatogenesis in non-obstructive azoospermia: a systemic review vol.12, pp.1, 2018, https://doi.org/10.1186/s13287-021-02295-9