DOI QR코드

DOI QR Code

New protocol for the indirect regeneration of the Lilium ledebourii Bioss by using bulb explants

  • Ghanbari, Sina (Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol) ;
  • Fakheri, Barat Ali (Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol) ;
  • Naghavi, Mohammad Reza (Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran) ;
  • Mahdinezhad, Nafiseh (Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol)
  • Received : 2018.05.14
  • Accepted : 2018.06.11
  • Published : 2018.06.30

Abstract

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

Keywords

References

  1. Abu-Romman S, Suwwan, M, and Al-Ramamneh, E. (2013) The influence of plant growth regulators on callus induction from hypocotyls of cucumber (Cucumis sativus L.). Advances in Environmental Biology, 7(2):339-343
  2. Arzate FAM, Nakazaki T, Qkumato T, and Tanisaka T. (1997) Efficient callus induction and plant regeneration from filaments with anther in lily (Lilium longiflorum Thunb). Plant Cell Rep 16(12):836-840 https://doi.org/10.1007/s002990050330
  3. Azadi P. (2003) Effect of growth regulators, sucrose concentration and scale pieces on micropropagation of Chelcheragh lily (Lilium ledebourii) in spring season. Proceedings of the 2nd Sci and Applied Seminar on Ornamental Plants, P 43
  4. Azadi P, and Khosh-Khui M. (2007) Micropropagation of Llilium ledebourii (Baker) Bioss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments. Electronic Journal of Biotech 10(4):582-591
  5. Bacchetta L, Remotti, PC, Bernardini C, and Saccardo F. (2003) Adventitious shoot regeneration from leaf explants and stem nodes of Lilium. Plant Cell Tissue Organ Cult. 74:37-44 https://doi.org/10.1023/A:1023315321931
  6. Bagheri A, Saffari M (2003) In Vitro Culture of Higher Plants. Translate. 4th Edition. Ferdowsi University of Mashhad. 406 pp
  7. Bakhshaie M, Babalar M, Mirmasoumi M, and Khalighi A. (2010) Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss, an endangered species. Plant Cell Tissue Organ Cult. 102:229-235 https://doi.org/10.1007/s11240-010-9726-4
  8. Chang C, Chen CT, Tasi YC, and Chang WC. (2000) A tissue culture protocol for propagation of a rare plant, Lilium speciosum Thunb. var. gloriosoides Baker. Botari cal Bulletin of Academic Sinica 41:139-142
  9. Chawla HS. (2000) Introduction to Plant Biotechnology, Science Publishers, pp 368
  10. Chawla HS. (2002) Introduction to Plant Biotechnology, Science Publishers, pp 538
  11. Cig A, and Basdogan G. (2015) In vitro propagation techniques for some geophyte ornamental plants with high economic value, International Journal of Secondary Metabolite. 2(1):27-49
  12. Eudes F, Acharya S, Laroche A, Selinger LB, and Cheng KJ. (2003) Novel method to induce direct somatic embryogenesis, secondary embryogenesis and regeneration of fertile green cereal plants. Plant Cell, Tissue and Organ Culture, 73: 147-157 https://doi.org/10.1023/A:1022800512708
  13. Gholami M. (2007) Micropropagation of of inverted tulip (Fritillaria imperialis L.). MSc. Thesis, University of Avicenna. Hamedan, Iran. Pp 69
  14. Ilahi I, Bano S, Jabeen M, and Rahim F. (2005) Micropropagation of rice (Oryza sativa L. cv. SWAT-II) through somatic embryogenesis. Pak J Bot 37(2):237-242
  15. Islam MM., Wahed SA., Khan SAKU. (2004). Studies on callus induction and regeneration from dehusked rice (Oryza sativa L.) seeds. Plant Tissue Cult 14(2):155-160
  16. Jin S, Wang J, Wang X, and Liu S. (2014) Direct and indirect shoot and bulblet regeneration from cultured leaf explants of Lilium pumilum, an endangered species, In Vitro Cellular and Developmental Biology-Plant, 50:69-75 https://doi.org/10.1007/s11627-013-9573-1
  17. Kamo K, and Hee Han B. (2012) Optimized groeth and plant regeneration for callus of Lilium Longflorum 'Nellie White'. Floriculture and Ornamental Biotech 6(2):94-98
  18. Kanchanapoom K, Ponpiboon T, Wirakiat W, and Kanchanapoom K. (2011) Regeneration of lily (Lilium longiflorum Easter lily) by callus derived from leaf explants cultured in vitro. Sci Asia 37:373-376 https://doi.org/10.2306/scienceasia1513-1874.2011.37.373
  19. Lomax TL, Muday GK, and Ruberty PH. (1995) Auxin transport. In plant hormones: physiology,biochemistry and molecular in plant hormones: physiology, biochemistry and molecular biol (Davies, P.J., ed.). Dordrecht: Kluwer Academic Publishers pp 509-530
  20. Mohamadi-Dehcheshmeh M. (2005) Using tissue culture techniques in the reproduction of endangered species of theinverted tulip native to Iran. MSc. Thesis, Tehran University. Tehran, Iran
  21. Mori S, Adachi Y, Horimoto S, Suzuk A, and Nakano M. (2005) Callus formation and plant regeneration in various Lilium species and cultivars. In Vitro Cell Dev Biol - Plant 41:783-788 https://doi.org/10.1079/IVP2005707
  22. Murashige T, and Skoog F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Phisiol planta 15:97-473
  23. Nhut DT. (2003) The control of in vitro direct main stem formation of Lilium longiflorum derived from receptacle culture and rapid propagation by using in vitro stem nodes. Plant Growth Regulation 40(2):179-184 https://doi.org/10.1023/A:1024263803684
  24. Padasht Dahkaei MN. (2005) The investigation of different methods for culturing and propagarion of Chelcheragh lily (Lilium ledebourii), native of Iran, and its introduction possibility as a new floricultural crop. Ph. D. Thesis, Islamic Azad University, Science and Research Unit, Tehran, Iran pp 120
  25. Pierik, RLM. (1987). In vitro Culture of Higher Plants. springer, 311 PP
  26. Rout G, Mohapatra A, and Mohan S. (2006) Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects Biotech Adv 24:531-560 https://doi.org/10.1016/j.biotechadv.2006.05.001
  27. Saetiew K, and Umamanit T. (2015) Micropropagation of Lilium formolongo via leaf explants. Journal of agricultural Technol 11(4):855-862
  28. Simmonds JA, and Cumming BG. (1976). Propagation of Lilium Hybrids. II. Production of plantlets from bulb-scale callus culture for increased propagation rates. Scientia Hortic 5:161-170 https://doi.org/10.1016/0304-4238(76)90078-9
  29. Skoric M, Zivkovic S, Savic J, Siler B, Sabvoljevic A, Todorovic., Sladjana.,and Grubisic D. (2012) Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis. African journal of Biotech 11(8):1862-1867 https://doi.org/10.5897/AJB11.2076
  30. Sun XC, Guang FC, Huang CY, and Liu DY. (2008) Study on the subculture, root induction and transplanting technique of low lily. Hebei J. For. Orchard Res. 23:211-213
  31. Swarup R, Bennett M (2003) Auxin transport: the fountain of life in plants; Dev Cell 5:824-826 https://doi.org/10.1016/S1534-5807(03)00370-8
  32. Tang YP, Liu XQ, Wahiti Gituru R, and Chen LQ. (2010) Callus Induction and Plant Regeneration from in vitro cultured leaves, Petioles and scales of Lilium Leucanthum (Baker) Baker. Biotechnology and Biotechnological Equipment 24(4):2071-2076 https://doi.org/10.2478/V10133-010-0077-4
  33. Thadavong S, Sripichitt P, Wongyai W, and Jompuk P. (2002) Callus induction and plant regeneration from mature embryos of glutinous rice (Oryza sativa L.) cultivar TDK1. Kasetsart Journal 36(4):334-344
  34. Varshney A, Dhawan V, and Srivastava PSA. (2000) protocol for in vitro mass propagation of Asiatic hybrids of lily through liquid stationary culture. In Vitro Cell. Dev. Biol. Plant 36:383-391
  35. Zaidi MA, Narayanan M, Sardana R, Taga I, Postel S, Johns R, McNulty M, Mottiar Y, Mao J, Loit E, and Altosaar I. (2006) Optimizing tissue culture media for efficient transformation of different indica rice genotypes. Agronomy Res 4(2):563-575