Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Microtuberization and Acclimatization in the Dioscorea cayenensis Thunb. by the Carbon Source

탄소급원에 의한 얌의 기내 비대근 형성과 순화

  • Lee, Na Nyum (Forest Biotechnology Division, National Institute of Forest Science (NIFoS)) ;
  • Kim, Ji Ah (Forest Biotechnology Division, National Institute of Forest Science (NIFoS)) ;
  • Kim, Yong Wook (Special Forest Products Division, National Institute of Forest Science (NIFoS)) ;
  • Kim, Tae Dong (Forest Biotechnology Division, National Institute of Forest Science (NIFoS))
  • 이나념 (국립산림과학원 산림생명공학연구과) ;
  • 김지아 (국립산림과학원 산림생명공학연구과) ;
  • 김용욱 (국립산림과학원 산림소득자원연구과) ;
  • 김태동 (국립산림과학원 산림생명공학연구과)
  • Received : 2017.11.18
  • Accepted : 2018.06.06
  • Published : 2018.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this experiment, we investigated the effects of various carbon sources and concentrations on the microtuber induction and acclimatization of the yam (Dioscorea cayenensis). First, the effects of the in vitro carbon sources and concentrations on the microtuber induction were examined. The highest efficiency of the microtuber induction was obtained in the 7% sucrose treatment, whereas the glucose treatment shows no effect on the microtuber formation. Secondly, the effects of the survival rate and the microtuber formation rate after the acclimatization were examined. The diameter (6.1 mm) and fresh weight (0.5g) of the tuberous root are the highest in the pretreatment of the 7% sucrose. Although the survival rate of the pretreatment of the low concentration sucrose (3% sucrose) is 100 %, the growth and development were inhibited. These results suggest the 7% sucrose treatment is appropriate for the yam microtuber formation and acclimatization. In addition, this protocol could be used for the propagation of virus- or disease-free clones and the multiplication of elite yam cultivars.

이 실험에서 우리는 얌의 기내 비대근 유도와 순화에 영향을 미치는 탄소급원의 효과를 연구하였다. 첫 번째로 기내 비대근 유도를 위한 탄소원의 종류와 농도의 효과를 실험하였다. 7% sucrose처리에서 기내 비대근 유도 효율이 가장 높았으며, glucose처리에서는 기내비대근이 형성되지 않았다. 두 번째로 기내식물체의 순화 후 생존율과 비대근 형성율을 실험하였다. 순화 후 형성된 비대근의 직경(6.1 mm)과 생중량(0.5 g)은 7% sucrose에서 생육한 기내식물체에서 가장 높았다. 저농도(3%)의 sucrose처리에서 생육한 기내식물체의 순화 후 생존율은 100%였으나 식물체의 생육과 발달이 억제되었다. 이러한 결과는 얌의 기내 비대근 형성과 순화를 위해 7% sucrose처리가 적정하며 이러한 기술은 얌의 바이러스 및 병 저항성 클론과 우수 개체의 번식을 위해 적용이 가능하다.

Keywords

References

  1. Alizadeh S, Mantell SH, MariaViana A (1998) In vitro shoot culture and microtuber induction in the steroid yam Dioscorea composite Hemsl. Plant Cell Tissue Organ Cult 53:107-112 https://doi.org/10.1023/A:1006036324474
  2. Ascough GD, Erwin JE, van Staden J (2008) Reduced temperature, elevated sucrose, continuous light and gibberellic acid promote corm formation in Watsonia vanderspuyiae. Plant Cell Tissue Organ Cult 95:275-283 https://doi.org/10.1007/s11240-008-9441-6
  3. Balogun M, Fawole I, Ng S, Ng N, Shiwachi H, Kikuno H (2006) Interaction among cultural factors in microtuberization of white yam (Dioscorea rotundata). Trop Sci 46:55-59 https://doi.org/10.1002/ts.61
  4. Chen FQ, Fu Y, Wang DL, Gao X, Wang L (2007) The effect of plant growth regulators and sucrose on the micropropagation and microtuberization of Dioscorea nipponica Makino. J Plant Growth Regul 26:38-45 https://doi.org/10.1007/s00344-005-0147-2
  5. Conner AJ, Falloon PG (1993) Osmotic versus nutritional effects when rooting in vitro asparagus minicrown on high sucrose media. Plant Sci 89:101-106 https://doi.org/10.1016/0168-9452(93)90175-Y
  6. Feng F, Ye CH, Li YZ, Xu WF (2007) Effects of growth regulators, carbon sources and photoperiod on in vitro formation and growth and development of microtubers of Dioscorea fordii Prain et Burk. Plant Physiol Commun 43:1045-1049
  7. Huang CH, Chung JP (2011) Efficient indirect induction of protocorm-like bodies and shoot proliferation using field-grown axillary buds of a Lycaste hybrid. Plant Cell Tissue Organ Cult 106:31-38 https://doi.org/10.1007/s11240-010-9890-6
  8. International Institute for Tropical Agriculture (IITA) and Natural Resources Institute (NRI) (2006) "On the bright side of yam production" Retrieved on January 29, 2009 from: www.new-ag.info/06-3/develop/dev03.html
  9. John JL, Courtney WH, Decoteau DR (1993) The influence of plant growth regulators and light on microtuber induction and formation in Dioscorea alata L. cultures. Plant Cell Tissue Organ Cult 34:245-252 https://doi.org/10.1007/BF00029713
  10. Kim Ji Ah, Heung Kyu Moon, Yong Eui Choi (2013) Microtuber formation from in vitro Codonopsis lanceolata plantlets by sugar. J Plant Biotechnol 40:147-155 https://doi.org/10.5010/JPB.2013.40.3.147
  11. Li M., J Li, W Liu, L Liu, J Lu, J Niu, X Liu and Q Yang (2014). A protocol for In vitro production of microtubers in Chinese yam (Dioscorea opposita). Biosci Biotechnol Biochem 78:1005-1009 https://doi.org/10.1080/09168451.2014.912119
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Ng CY, Saleh NM (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm - like bodies. Plant Cell Tissue Organ Cult 105:193-202 https://doi.org/10.1007/s11240-010-9851-0
  14. Olivier KA, Konan KN, Anike FN, Agbo GN, Dodo HW (2012) In vitro induction of minitubers in yam (Dioscorea cayenensis-D. rotundata complex). Plant Cell Tissue Organ Cult 109:179-189 https://doi.org/10.1007/s11240-011-0084-7
  15. Ovono PO, Kevers C, Dommes J (2007) Axillary proliferation and tuberisation of Dioscorea cayenensis-D. rotundata complex. Plant Cell Tissue Organ Cult 91:107-114 https://doi.org/10.1007/s11240-007-9238-z
  16. Rayirath UP, Lada RR, Caldwell CD, Samuel K, Asiedu SK, Kevin J, Sibley KJ (2011) Role of ethylene and jasmonic acid on rhizome induction and growth in rhubarb (Rheum rhabarbarum L.). Plant Cell Tissue Organ Cult 105:253-263 https://doi.org/10.1007/s11240-010-9861-y
  17. Sheela Chandra, Rajib Bandopadhyay, Vijay Kumar, Ramesh Chandra (2010) Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnol Lett 32:1199-1205 https://doi.org/10.1007/s10529-010-0290-0
  18. Shou Yong Choy Ng (1988) In vitro tuberization in white yam (Dioscorea rotundata Poir). Plant Cell Tissue Organ Cult 14:121-128 https://doi.org/10.1007/BF00041185
  19. Thorpe T, Stasolla C, Yeung EC, de Klerk GJ, Roberts A, George EF (2007) Plant propagation by tissue culture 3rd edition, p115-174. In: E.F. George MA Hall, and GJ de Kler (eds). Chapter 4. The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems. Springer Dordrecht, Netherlands
  20. Torres-Moran MI, Martha Escoto-Delgadillo, Sandy Molina-Moret, Diana M. Rivera-Rodriguez, Ana P. Velasco-Ramirez, Diogenes Infante, Liberato Portillo (2010) Assessment of genetic fidelity among Agave tequilana plants propagated asexually via rhizomes versus in vitro culture. Plant Cell Tissue Organ Cult 103:403-409 https://doi.org/10.1007/s11240-010-9777-6
  21. Uchendu EE, Shukla M, Saxena PK, Joachim E. R. Keller (2016) Cryopreservation of potato microtubers: the critical roles of sucrose and desiccation. Plant Cell Tissue Organ Cult 124:649-656 https://doi.org/10.1007/s11240-015-0916-y
  22. Yan H, Yang L, Li Y (2011) Axillary shoot proliferation and tuberization of Dioscorea fordii Prain et Burk. Plant Cell Tissue Organ Cult 104:193-198 https://doi.org/10.1007/s11240-010-9818-1
  23. Yuki M, Michihiko S, Kenji K , Nobukazu N , Mari S, Misaki I, Yuichi K, Yoshiyuki M, Hiroyuki K, Kanako K, Tsutomu K, Akihito W, Hajime O, Hiroshi I, Nobuhiro M, Kunihiro N, Yu S & Takuji S (2015) The radish genome and comprehensive gene expression profile of tuberous root formation and development. Scientific Reports 5:10835, DOI: 10.1038/srep10835