Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Beech-wood Creosote on Intestinal Microflora in Rat

너도밤나무 크레오소트가 흰 쥐의 장내 미생물 변화에 미치는 영향

  • Kim, Jeong A (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Yu, Da Yoon (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Kim, In Sung (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Lee, Chul Young (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Jeong, Dong Kee (Faculty of Biotechnology, Jeju National University) ;
  • Lee, Sang Suk (Department of Animal Science and Technology, Sunchon National University) ;
  • Choi, In Soon (Department of Biological Science, Silla University) ;
  • Cho, Kwang Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology)
  • 김정아 (경남과학기술대학교 동물소재공학과) ;
  • 유다윤 (경남과학기술대학교 동물소재공학과) ;
  • 김인성 (경남과학기술대학교 동물소재공학과) ;
  • 이철영 (경남과학기술대학교 동물소재공학과) ;
  • 정동기 (제주대학교 생명공학부) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 최인순 (신라대학교 생명과학과) ;
  • 조광근 (경남과학기술대학교 동물소재공학과)
  • Received : 2018.03.06
  • Accepted : 2018.07.24
  • Published : 2018.07.30
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Abstract

Diarrhea is one of the main disorders which cause the highest level mortality of the post-weaning economic animal. Beech-wood creosote has been used as a traditional anti-diarrheic medicament for a long time. The present study was conducted to investigate the effects of dietary supplementation of Beech-wood on growth performance and intestinal microbiota in rats. Twelve 4-week-old rats were randomly assigned to one of four dietary groups and fed a basal diet supplemented with none (CON), 0.5% apramycin (ANTI), 0.4% creosote (Creo 0.4), or 0.8% creosote (Creo 0.8) for 4 weeks following 1 week of adaptation period to the respective diet. Average daily gain was not influenced by the dietary treatment whereas average daily feed intake was greatest for the Creo 0.8 group. In the intestinal microbiota at the level of the phylum, the percentage of Firmicutes bacteria decreased but Bacteroidetes increased in the Creo 0.8 group vs. Control, which resulted in a decreased F/B ratio for the former (p<0.05). Moreover, the percentage of Lachnospiraceae was greater at the level of the family for the Creo 0.8 group than for Control, but the percentages of Turicibacter and Clostridium disporicum were less in the former (p<0.01) at the genus and species levels, respectively. Collectively, the present results indicate that dietary supplementation of creosote increases the feed intake and also influence the intestinal microbiota in rats.

설사는 경제동물의 이유시기 폐사율을 일으키는 가장 높은 요인이다. 크레오소트는 전통 의약품으로 오랜 세월동안 지사제로 사용되어 왔다. 본 연구는 흰 쥐 모델에서 크레오소트 급여가 동물의 성장 효율 및 장내 미생물에 미치는 영향을 구명할 목적으로 수행되었다. 4주령의 수컷 흰쥐 24마리를 임의로 대조구, 항생제 그룹, 크레오소트 0.4% 그룹, 크레오소트 0.8% 그룹으로 배치하였다. 대조구는 기초사료, 항생제 그룹은 apramycin 0.5%, 크레오소트 그룹은 크레오소트 0.4%와 0.8% 수준으로 하여 예비시험 기간 1주일, 본시험 기간 4주일 동안 급여하였다. 일당증체량은 실험구간 차이가 없었으나, 사료 섭취량은 Creo 0.8 그룹에서 유의적으로 증가하였다(p<0.05). 장내 미생물에 대한 문(phylum) 수준 분석 결과 Creo 0.8 그룹에서 Firmicutes가 감소하고 Bacteroidetes가 증가하여 F/B비율을 감소시키는 것으로 나타났다(p<0.05). 과(family) 수준에서는 Lachnospiraceae가 크레오소트 0.8% 수준에서 증가되었으며(p<0.01), 속(genus) 수준에서는 Turicibacter가 감소되었다(p<0.01). 종(species) 수준에서는 Clostridium disporicum이 감소되었다(p<0.01). 이상의 결과는 쥐에 크레오소트 급여는 사료 섭취량을 증가시키고 장내 미생물의 변화에 영향을 미칠 수 있다는 것을 시사한다.

Keywords

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